GENOTYPIC UNIFORMITY OF ACYL-COA SYNTHETASE LONG-CHAIN FAMILY MEMBER 1 GENE AND MILK QUALITY PARAMETERS IN INDIGENOUS RED KANDHARI COWS
DOI:
https://doi.org/10.48165/abr.2026.28.01.36Keywords:
Genetic association, milk, monomorphic, polymerase chain reaction, Red KandhariAbstract
The present study explored polymorphism in ACSL1 gene, a candidate gene involved in fatty acid metabolism and milk fat synthesis, in thirty lactating Red Kandhari cattle. Two reported SNP regions, g.35446C>T and g.35941G>A, were targeted for genotyping. The polymerase chain reaction for SNP g.35446C>T, and g.35941G>A yielded PCR amplicon of 148 and 142 bp, respectively. SSCP analysis revealed no mobility variation, and subsequent Sanger sequencing confirmed monomorphic patterns with homozygous CC and GG genotypes, respectively. These findings indicated that the selected ACSL1 regions are conserved in this indigenous breed. Although milk trait data—including fat percentage, protein percentage, lactose, solid-not-fat, somatic cell score, and milk yield—were collected, the absence of polymorphism precluded genotype phenotype association analysis. Documenting monomorphic loci is scientifically relevant as it establishes a genetic baseline for Red Kandhari cattle and contributes to the broader understanding of breed-specific genetic diversity. This study represents the first report of ACSL1 gene monomorphism in Red Kandhari cattle. The lack of polymorphism limited association analysis with milk traits and the results underscore the importance of expanding genetic studies to include additional SNPs, larger populations, and comparative analyses across breeds.
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Bernard, L., Rouel, J., Leroux, C., Ferlay, A., Faulconnier, Y., Legrand, P., et al. (2005). Mammary lipid metabolism and milk fatty acid secretion in Alpine goats fed vegetable lipids. Journal of Dairy Science, 88, 1478–1489.
Bionaz, M., & Loor, J. J. (2008). Gene networks driving bovine milk fat synthesis during the lactation cycle. BMC Genomics, 9, 366. https://doi.org/10.1186/1471-2164-9-366
Brym, P., Kamiński, S., & Rusc, A. (2004). New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in Black-and-White and Jersey cattle. Journal of Applied Genetics, 45, 445–452.
Chandrakar, C., Kumar, P., Shakya, S., Jaiswal, S. K., Monika, & Wasist, U. (2017). Raw milk composition of crossbred cows and correlation between milk constituents in selected districts of Chhattisgarh, India. International Journal of Bio-resource and Stress Management, 8, 811–814.
Čítek, J., Brzáková, M., Hanusová, L., Hanuš, O., Večerek, L., Samková, E., et al. (2022). Somatic cell score: Gene polymorphisms and other effects in Holstein and Simmental cows. Animal Bioscience, 35, 13–21.
Dubey, P. K., Dubey, S., Mishra, S. K., Arora, R., Patel, J., Singh, K. P., et al. (2017). PCR-SSCP analysis of MDGI gene and its association with milk production traits in river buffalo (Bubalus bubalis). Research in Veterinary Science, 115, 307–309.
Ellis, J. M., Li, L. O., Wu, P. C., Koves, T. R., Ilkayeva, O., Stevens, R. D., et al. (2010). Adipose acyl-CoA synthetase-1 directs fatty acids toward β-oxidation and is required for cold thermogenesis. Cell Metabolism, 12, 53–64.
Ghafoor, M. A. (1987). History, origin and breed characteristics of Red Kandhari cattle (pp. 18–24). In Proceedings of the Symposium on Development of Deoni and Red Kandhari Cattle Breed. Marathawada Agricultural University.
Grevengoed, T. J., Martin, S. A., Katunga, L., Cooper, D. E., Anderson, E. J., Murphy, R. C., et al. (2015). Acyl-CoA synthetase 1 deficiency alters cardiolipin species and impairs mitochondrial function. Journal of Lipid Research, 56, 1572–1582.
Kale, D. S., & Yadav, B. R. (2009). Single strand conformation polymorphism (SSCP) analysis using nondenaturing PAGE. Protocol Online. https://www.protocol-online.org/prot/ProtocolsSingle-Strand-Conformation-Polymorphism--SSCP--Analysis-by-Nondenaturing-PAGE-3468.html
Liang, S. S., Pang, C. Y., Deng, T. X., Ma, X. Y., Lu, X. R., Duan, A. Q., et al. (2020). Expression of ACSL1 and its effect on expression involved in fatty acid metabolism in buffalo. Chinese Journal of Animal Science, 56, 41–45.
Liang, Y., Gao, Q., Zhang, Q., Arbab, A. A. I., Li, M., Yang, Z., & Mao, Y. (2020). Polymorphisms of the ACSL1 gene influence milk production traits and somatic cell score in Chinese Holstein cows. Animals, 10, 2282. https://doi.org/10.3390/ani10122282
Mahe, M. F., & Grosclaude, F. (1993). Polymorphism of β-casein in the Creole goat of Guadeloupe: Evidence of a null allele. Genetics Selection Evolution, 25, 403–408.
Muoio, D. M., Lewin, T. M., Wiedmer, P., & Coleman, R. A. (2000). Acyl-CoAs are functionally channeled in liver: Potential role of acyl-CoA synthetase. American Journal of Physiology-Endocrinology and Metabolism, 279, E1366–E1373.
Pundir, R. K., & Singh, P. K. (2008). Status, characteristics and performance of Red Kandhari cattle breed in its native tract. The Indian Journal of Animal Sciences, 78, 56–61.
Sadan, T., Valsalan, J., Venketachalapathy, T., & Aravindakshan, T. V. (2021). PRL and B4GALT-1 gene polymorphism and their association with milk production traits in crossbred cattle of Kerala. Indian Journal of Animal Sciences, 90, 1383–1386.
Sambrook, J., & Russell, D. W. (2001). Molecular cloning: A laboratory manual (3rd ed.). Cold Spring Harbor Laboratory Press.
Schalm, O. W., Carroll, E. J., & Jain, N. C. (1971). Bovine mastitis. Lea & Febiger.
Siddiqui, M. F., Auradakar, S. K., & Dhumal, M. V. (2000). Characteristics and development of Red Kandhari cattle breed. Marathawada Agricultural University.
Singh, A., Malla, W. A., Kumar, A., Jain, A., Thakur, M. S., Khare, V., et al. (2023). Review: Genetic background of milk fatty acid synthesis in bovines. Tropical Animal Health and Production, 55, 328. https://doi.org/10.1007/s11250-023-03754-6
Soupene, E., & Kuypers, F. A. (2008). Mammalian long-chain acyl-CoA synthetases. Experimental Biology and Medicine, 233, 507–521.
Soupene, E., Dinh, N. P., Siliakus, M., & Kuypers, F. A. (2010). Activity of the acyl-CoA synthetase ACSL6 isoforms: Role of the fatty acid gate-domains. BMC Biochemistry, 11, 18. https://doi.org/10.1186/1471-2091-11-18
Tirfie, F. W. (2023). A review of genetic and non-genetic parameter estimates for milk composition of cattle. Animal and Veterinary Sciences, 11, 64–70.
Widmann, P., Nuernberg, K., Kuehn, C., & Weikard, R. (2011). Association of an ACSL1 gene variant with polyunsaturated fatty acids in bovine skeletal muscle. BMC Genetics, 12, 96. https://doi.org/10.1186/1471-2156-12-96
Ye, J., Coulouris, G., Zaretskaya, I., Cutcutache, I., Rozen, S., & Madden, T. L. (2012). Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics, 13, 134. https://doi.org/10.1186/1471-2105-13-134
Zhao, Z. D., Tian, H., Jiang, Y. Y., Shi, B. G., Liu, X., Li, X. P., et al. (2019). Polymorphisms of ACSL1 gene promoter and their association analysis with milk quality traits in yak (Bos grunniens). Journal of Agricultural Biotechnology, 27, 1596–1603.
