Bio-efficacy of bioagents against Pectobacterium carotovorum subsp. carotovorum
DOI:
https://doi.org/10.48165/jefa.2026.21.2.20Keywords:
Pectobacterium carotovorum, carrot, soft rot disease, bioagents, inhibitionAbstract
In vitro efficacy of four chemical substance and five antibiotics were evaluated against Pectobacterium carotovorum subsp. carotovorum the causal agent of carrot soft rot disease. Pectobacterium carotovorum subsp. carotovorum was isolated from diseased carrot sample by serial dilution technique and different biochemical and pathogenicity test were performed to confirm the bacterial species. Bioagents viz., Pseudomonas fluorescens (UAS, Raichur), Pseudomonas fluorescens (Ap-40), Bacillus subtilis, Bacillus amyloliquifaciense, Bacillus thuringenesis, Trichoderma asperillum (UAS, Raichur) and probiotics like, Lactobacillus sp. MYSVA- 20, Lactobacillus sp. MYSVA- 21 and Lactobacillus sp. MYSVA- 23 were tested for their potentiality to suppress the bacterium by streak plate method. Data were recorded at 96 hour after incubation and results showed that among the different bioagents Among the biocontrol agents P. fluorescens (BC lab) was found significantly superior over all other treatments in suppression of the pathogen with an inhibition zone of 12.66 mm. Followed by the next best effective biocontrol agent as B. subtilis (9.00) and P. fluorescens- AP05 (7.16 mm). Significantly least inhibition was recorded was observed by B. amyloliquifaciense (4.16 mm).
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Agrios, G. N. 2006. Bacterial soft rots. In: Plant Pathology. 5th edition, Academic Press San Diego, p. 656.
Akbar, A., Din, S., Ahmad, M., Khan, G. and Alam, S. 2015. Effect of phytobiocides in controlling soft rot of tomato. J. Nat. Sci. Res., 4(11): 99-102.
Alabouvette C, Olivain C, Steinberg C (2006) Biological control of plant diseases: the European situation. Eur J Plant Pathol 114:329- 341.
Alavi, G, B. S., Ahmadzadeh, M., Behboudi, K. and Jamali, S., 2012, Biocontrol of rhizome soft rot (Pectobacterium carotovorum) on valerian by Pseudomonas spp. under in vitro and greenhouse conditions. J. Agric. Technol., 8(6):1913- 1923.
Anonymous, 2002, Studies on recently occurring bacterial diseases of various crops in Haryana. Technical Report. Chowdhary Cheran Singh Heryana Agril. Univ., Hisar, India, 4- 6.
Arscott, S. A. and Tanumihardjo, S. A. 2010. Carrots of many colors provide basic nutrition and bioavailable phytochemicals acting as a functional food. Compr. Rev. Food Sci. Food Saf., 9: 223-239.
Barbara, M., Philipp, W., Simon and Warren, H. G. 1992. Assessing susceptibility of carrot roots to bacterial soft rot. Hort. Sci., 27(9): 1020-1022.
Bradbury, J. F. 1986. Guide to Plant Pathogenic Bacteria. CAB Int. Mycol. Inst., New York, USA, p. 332.
Cowan, S. T. 1974. Cowan and steel’s manual for the identification of medical bacteria, 2nd ed. Cambridge University Press, Cambridge. 67-83.
Cruz, A. T., Cazacu, A. C. and Allen, C. H., 2007, Pantoea agglomerans, a plant pathogen causing human disease. J. Clin. Microbiol., 45(6): 1989- 1992.
Dipak, T. N., Suresh, G. B., Shailesh, P., Gawande, Asit, K. M. and Sathish, A. R. 2013. Characterization of a bacterial collar and rhizome rot of banana (Musa paradisiaca) caused by strains of Erwinia Chrysanthemi pv. paradisica. J. Appl. Nat. Sci., 5(2): 435- 441.
Fhoula, I., Najjari, A., Turki, Y., Jaballah, S., Boudabous, A. and Ouzari, H., 2013, Diversity and antimicrobial properties of lactic acid bacteria isolated from rhizospheric of olive trees and desert truffles of Tunisia. Biomed. Res. Int., 45(8): 2003- 2004.
Hind, F., Terta, M., Amdan, M., El- Hassan, A., Mustapha, M., Ennaji and Rajaa, A. M. 2015. Phenotypic and genotypic diversity of Pectobacterium carotovorum subsp. carotovorum causing soft rot disease of potatoes in Morocco. European J. Plant Pathol., 28(3): 1-12.
Irdasafni, S. P., 2003, The application of antagonistic bacteria Pseudomonas fluorescens to control soft rot disease (Erwinia carotovora) on potato crops. Fakultas Pertanian Univarsitas Sumatera Utara. 69- 80.
Issazadeh, K., Rad, S. K., Zarrabi, S. and Rahimibashar, M. R., 2012, Antagonism of
Bacillus species against Xanthomonas campestris pv. campestris and
Pectobacterium carotovorum subsp. carotovorum. African Journal of
Microbiology Research., 6(7):1615-1620.
Jones, A. L., McManus, P. S and Chiou, C. S., 1996, Epidemiology and genetic diversity of streptomycin resistance in E. amylovora in Michigan. Acta Hort., 338:333- 340.
Khair, A. H. and Haggy, K. H. E., 2007, Applications of some bactericides and bioagents for controlling the soft rot disease in potato. Res. J. Agric. Biol. Sci., 3(5):463-473.
Khosro, I., Sara, K. R., Saeed, Z. and Mohammad, R. R., 2012, Antagonism of Bacillus species against Xanthomonas campestris pv. campestris and Pectobacterium carotovorum subsp. carotovorum. African J. Microbiol. Res., 6(7): 1615- 1620.
Kovacs, N. 1956. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature, 178: 703.
Larka, B. S., 2004, Integrated approach for the management of soft rot (Pectobacterium carotovorum subsp. carotovorum) of radish (Raphanus sativus) seed crop. Haryana J. Agron., 20:128- 129.
Nicolle, C., Simon, G., Rock, E., Amouroux, P. and Remesy, C. 2004. Genetic variability influences carotenoid, vitamin, phenolic, and mineral content in white, yellow, purple, orange, and dark-orange carrot cultivars. J. Am. Soc. Hortic. Sci. 129: 523-529.
Rahman, M. M., Eaqub Ali, M., Khan, A. A., Hashim, U., Akanda, A. M. and Hakim, M. A., 2012, Characterization and identification of soft rot bacterial pathogens in Bangladeshi potatoes. African J. Microbiol. Res., 6(7):1437- 1445.
Rubatzky, V. E., Quiros, C. F. and Simon, P. W. 1999. Carrots and related vegetable Umbelliferae, CABI, University of Wisconsin.
Salem, E. A. and Shafea, Y. M., 2018, Biological control of potato soft rot caused by Erwinia carotovora subsp. carotovora. Egyptian J. Biol. Pest Control, 28:94
Salle, A. J., 1961, Fermentation of carbohydrates and related compounds. In: Laboratory manual on fundamental principles of bacteriology. McGraw Hill Book Comp. Inc., New York, pp. 94- 98.
Sangeetha, V., Harish, S., Thiruvudainambi, S. and Beaulah, A. 2020. Phenotypic and biochemical characterization of Pectobacterium carotovorum subsp carotovorum, the Incitant of onion soft rot. Int. J. Curr. Microbiol. Appl. Sci., 9(12): 8-15.
Schaad, N. W., 1992, Xanthomonas. In: Laboratory Guide for Identification of Plant Pathogenic Bacteria. 2nd Ed. Int. Book Distributing Co. Lucknow, pp. 165.
Sneha, S. G. and Anuradha, S. N., 2017, Evaluation of quorum quenching
Bacillus spp. for their biocontrol traits against Pectobacterium carotovorum sub
sp. carotovorum causing soft rot. Biocatalysis Agric. Biotechnol., 9: 48- 57.
Snehalatharani, A., 2009, Studies on etiology and biocontrol of tip over disease of banana, M.Sc. (Agri.) Thesis, Uni. Agric. Sci., Bangalore, Karnataka, India, pp. 45.
Soumya, D. R. and Ravikumar, M. R., 2018, Molecular characterization of effective PGPRs from rhizosphere of banana against tip over disease caused by Erwinia carotovora subsp. carotovora. J. Pharma. Phytochem., 7(5): 2387- 2392.
Suslow, T. V., Schroth, M. N. and Isaka M. 1982. Application of a rapid method for gram differentiation of plant pathogenic and saprophytic bacteria without staining. American Phytopathological Soci., 10(1094): 72-91.
Thiyagarajan, V. 2016. Investigation on tip-over disease of banana caused by Erwinia carotovora subsp. carotovora (Jones) Holland. M. Sc. (Agri.) Thesis, Univ. Agric. Sci., Raichur. pp. 120.
Trias, R., Baneras, L., Montesinos, E. and Badosa, E., 2008, Lactic acid bacteria from fresh fruit and vegetables as biocontrol agents of phytopathogenic bacteria and fungi. Int. Microbiol., 11(4): 231- 236.
Vanneste, J. L., 2000, Fire blight The disease and causative agent Erwinia amylovora. CABI Publications, New York, pp 1- 370.
Vimal, B. M. and Anuradha, S. N., 2013, Characterization and differentiation of soft rot causing Pectobacterium caratovorum of Indian origin. European J. Plant Pathol., 138: 87- 102.
Wjohn, L., Masashi, N. and Kathrin, M., 2009, Stenotrophomonas maltophilia: an emerging opportunist human pathogen. The Lancet Infect. Dis., 9(5):312- 323.
Wulff, E. G., Van, J. W. L. and Hockenhull, J., 2003, The ability of the biological control agent Bacillus subtilis, strain BB, to colonize vegetable brassicas endophytically following seed inoculation. Plant Soil, 255:463- 474.
