Comparison of additives for enhancing shelf life of Azospirillum brasilense NAIMCC B 00896 biofertilizer
DOI:
https://doi.org/10.48165/jefa.2026.21.2.23Keywords:
Azospirillum brasilense, chemical additives, liquid biofertilizers, shelf life, cell viabilityAbstract
Global food security faces challenges from population growth and climate change, demanding sustainable biofertilizers. This study optimized liquid Azospirillum brasilense NAIMCC B 00896 formulations with additives. Trehalose (15 mM) + PVP (5%) best preserved Azospirillum viability (8.90 log CFU ml⁻¹ at 56 weeks), compliant to FCO limits. Post-storage plant growth promoting traits remained robust: N-fixation (25 mg N/g), IAA (38.93 μg/ml), ACC deaminase (18.71 μmol mg⁻¹ h⁻¹), siderophore (42.36%), P-solubilization (SI 2.67). Field trials of PR 126 enhanced rice yield 9.86% (81 q/ha), height (106 cm), tillers (310 m⁻²) compared to untreated control. These results validate a scalable, eco-friendly solutions.
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