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Indian Journal of Veterinary Sciences and Biotechnology
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Print ISSN 2394-0247 · Online ISSN 2395-1176
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Comparison Of Serological Methods For The Detection Of Brucella Abortus Antibodies In Sera From Infected Bovines

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A. S. Aher — Department of Microbiology Bombay Veterinary College, Parel, Mumbai 400 012.
S. P. Londhe — Department of Microbiology Bombay Veterinary College, Parel, Mumbai 400 012.
P. P. Mhase — Department of Microbiology Bombay Veterinary College, Parel, Mumbai 400 012.
A. S. Bannalikar — Department of Microbiology Bombay Veterinary College, Parel, Mumbai 400 012.
V. D. Dighe — Department of Microbiology Bombay Veterinary College, Parel, Mumbai 400 012.
Published 20 Dec 2011 Vol. 7 No. 3 (2012), pp. 14-16 ● Open access
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Abstract

The efficacy of rose Bengal plate test (RBPT) , standard tube agglutination test (STAT) and indirect enzyme linked immunosorbent assay (i-ELISA) in detection of bovine brucellosis was evaluated. The serum samples of 74 animals (68 cattle and 6 buffaloes )from different locations in Maharashtra were investigated for presence of Brucella antibodies by RBPT , STAT and i-ELISA. Overall observation of results of three serological tests employed in the present investigation for detection of bovine brucellosis suggests superiority of i-ELISA (56.75 %) over RBPT (54.05 %) and that of RBPT over STAT (47.29 %) in detecting the Brucella antibodies. 

KeywordsBrucellai-ELISASTATRBPT

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