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Home▶ Journals▶ Indian Journal of Veterinary Sciences and Biotechnology▶Archive▶Vol. 22 No. 5 (2026)▶Curcumin and L-Cysteine Supplementation in Tris Extender…
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Research Article

Curcumin and L-Cysteine Supplementation in Tris Extender Improves Freezability of Jamnapari Buck Semen

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Jayanth Marasandra Nanjappa — Deaprtment of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, ANDUAT, Kumarganj-224229, Ayodhya, UP, India
Bhoopendra Singh — Deaprtment of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, ANDUAT, Kumarganj-224229, Ayodhya, UP, India
Rajesh Kumar — Deaprtment of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, ANDUAT, Kumarganj-224229, Ayodhya, UP, India
Sushant Srivastava — Deaprtment of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, ANDUAT, Kumarganj-224229, Ayodhya, UP, India
Jaswant Singh — Deaprtment of Animal Genetics & Breeding, College of Veterinary Science & Animal Husbandry, ANDUAT, Kumarganj-224229, Ayodhya, UP, India
Shailendra Singh Chauhan — Deaprtment of Livestock Production and Management, College of Veterinary Science & Animal Husbandry, ANDUAT, Kumarganj-224229, Ayodhya, UP, India
Published 22 Aug 2026 Vol. 22 No. 5 (2026), pp. 19-22 ● Open access
❝ How to cite 🔗 https://doi.org/10.48165/ijvsbt.22.5.04
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Abstract

The purpose of the study was to assess how varying concentrations of curcumin and L cysteine HCl affected Jamnapari buck semen’s  ability to withstand cryopreservation-induced cryodamage. Four Jamnapari bucks, aged 2-3 years, participated in the current  investigation. Twenty-four ejaculates (six from each buck) were collected and diluted with Tris-Egg-Yolk-Citric acid-Fructose-Glycerol  extender containing no additives T0 (control), and that supplemented with Curcumin T1, T2 (Curcumin 1 & 2 µg/mL), and L-cysteine HCl  T3 & T4 (L cysteine 2 & 5 mM/L), respectively. The samples were cryopreserved in accordance with standard procedures. As compared  to T0 (control), the sperm motility, liveability and HOS reactivity were significantly (p<0.05) improved, whereas the sperm abnormality  and leakage of enzyme, transaminases (AST-ALT) and phosphatases (ACP-AKP) were significantly declined in T1, T2, T3 and T4 groups,  particularly in treatment groups T2 and T3. Thus, supplementing Jamnapari buck semen with 2 µg/mL curcumin and 2 mM/L L-cysteine  HCl in Tris extender appears to be helpful in reducing cryopreservation-induced cryodamage. 

 

KeywordsCryodamageCryopreservationCurcuminExtenderJamnapari buck semen

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