Curcumin and L-Cysteine Supplementation in Tris Extender Improves Freezability of Jamnapari Buck Semen
DOI:
https://doi.org/10.48165/ijvsbt.22.5.04Keywords:
Cryodamage, Cryopreservation, Curcumin, Extender, Jamnapari buck semenAbstract
The purpose of the study was to assess how varying concentrations of curcumin and L cysteine HCl affected Jamnapari buck semen’s ability to withstand cryopreservation-induced cryodamage. Four Jamnapari bucks, aged 2-3 years, participated in the current investigation. Twenty-four ejaculates (six from each buck) were collected and diluted with Tris-Egg-Yolk-Citric acid-Fructose-Glycerol extender containing no additives T0 (control), and that supplemented with Curcumin T1, T2 (Curcumin 1 & 2 µg/mL), and L-cysteine HCl T3 & T4 (L cysteine 2 & 5 mM/L), respectively. The samples were cryopreserved in accordance with standard procedures. As compared to T0 (control), the sperm motility, liveability and HOS reactivity were significantly (p<0.05) improved, whereas the sperm abnormality and leakage of enzyme, transaminases (AST-ALT) and phosphatases (ACP-AKP) were significantly declined in T1, T2, T3 and T4 groups, particularly in treatment groups T2 and T3. Thus, supplementing Jamnapari buck semen with 2 µg/mL curcumin and 2 mM/L L-cysteine HCl in Tris extender appears to be helpful in reducing cryopreservation-induced cryodamage.
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