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Home▶ Journals▶ Indian Journal of Veterinary Sciences and Biotechnology▶Archive▶Vol. 22 No. 5 (2026)▶Pathological Studies and Bacteriological Characterization…
Indian Journal of Veterinary Sciences and Biotechnology
Research Article

Pathological Studies and Bacteriological Characterization of  Streptococcus Species Associated with Pneumonia in Rabbits

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Katta Charith — Department of Veterinary Pathology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, Telangana, India
Soujanya Samala — Department of Veterinary Pathology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, Telangana, India
Boinepally Ramya — Department of Veterinary Pathology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, Telangana, India
Mule Venkata Krishna Reddy — Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, DSVCKV, Anjora, Durg-491001, Chhattisgarh, India
Kaluvai Sahithi — Department of Veterinary Pathology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, Telangana, India
Doggela Prathibha — Department of Veterinary Pathology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, Telangana, India
Nalabothula Laxmi Prasanna — Department of Veterinary Pathology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, Telangana, India
Published 22 Aug 2026 Vol. 22 No. 5 (2026), pp. 23-27 ● Open access
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Abstract

Respiratory infections are major cause of death in rabbits, which are often caused by bacterial pathogens. The present study was  conducted to determine the occurrence of streptococcal infections with pneumonia in rabbits through macroscopic, microscopic  pathological and bacteriological examination. A total of 45 rabbits that died between October 2025 and February 2026 were brought for  postmortem examination to the Department of Veterinary Pathology of the College in Rajendranagar, Hyderabad. Among them 29 were  bunnies (2 to 6 months) and 16 adult rabbits (6 months to 3 years). The necropsy was conducted as per protocol in clean environment.  9 adult rabbits and 20 bunnies showed pneumonia lesions on postmortem examination. Necropsy findings showed consolidation in  lungs, fibrinous exudation, abscess and pleuritis. Microscopic analysis of lung smears revealed Gram-positive cocci organized in chains.  Histopathology of lungs revealed suppuration, inflammatory cells infiltration, emphysema and haemorrhage. The organism was isolated  on Edwards medium and characterized by colony morphology and biochemical traits, including negativity for catalase, oxidase and  positivity for bile esculin. These findings confirmed presence of streptococcal species associated with pneumonic lesions in rabbits  and emphasize the importance of bacteriological and histopathological investigations for accurate diagnosis of respiratory infections.

KeywordsEdwards mediumGross pathologyHistopathologyRabbit pneumoniaStreptococcus spp

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  1. Chuard, C., & Reller, L. B. (1998). Bile-esculin test for presumptive identification of enterococci and streptococci: Effects of bile concentration, inoculation technique, and incubation time. Journal of Clinical Microbiology, 36(4), 1135–1136.
  2. Fernandez, M., Garcias, B., Duran, I., Molina-López, R. A., & Darwich, L. (2023). Current situation of bacterial infections and antimicrobial resistance profiles in pet rabbits in Spain. Veterinary Sciences, 10(5), 352.
  3. Herting, E., Sun, B., Jarstrand, C., Curstedt, T., & Robertson, B. (1997). Surfactant improves lung function and mitigates bacterial growth in immature ventilated rabbits with experimentally induced neonatal group B streptococcal pneumonia. Archives of Disease in Childhood: Fetal and Neonatal Edition, 76(1), F3–F8.
  4. Iorgoni, V., Iancu, I., Popa, I., Gligor, A., Orghici, G., Sicoe, B., & Herman, V. (2025). The first report of a pulmonary abscess due to Streptococcus intermedius in rabbits in Romania. Microorganisms, 13(4), 769.
  5. King, J. M., Roth-Johnson, L., Dodd, D. C., & Newsom, M. E. (2014). The necropsy book: A guide for veterinary students, residents, clinicians, pathologists, and biological researchers. The Internet-First University Press.
  6. Markey, B., Leonard, F., Archambault, M., Cullinane, A., & Maguire, D. (2013). Clinical veterinary microbiology (2nd ed.). Mosby Elsevier.
  7. Moody, A. M. (2016). Lesions in rabbits produced by streptococci from chronic alveolar abscesses. Journal of Infectious Diseases, 19(4), 515–525.
  8. Paray, A. A., Singh, M., Mir, M. A., & Kaur, A. (2023). Gram staining: A brief review. International Journal of Research Reviews, 10(9), 336–341.
  9. Quinn, P. J., Markey, B. K., Leonard, F. C., FitzPatrick, E. S., Fanning, S., & Hartigan, P. J. (2011). Veterinary microbiology and microbial disease (2nd ed.). Wiley-Blackwell.
  10. Rougier, S., Galland, D., Boucher, S., Boussarie, D., & Valle, M. (2006). Epidemiology and susceptibility of pathogenic bacteria responsible for upper respiratory tract infections in pet rabbits. Veterinary Microbiology, 115(1–3), 192–198.
  11. Sanders, E. R. (2012). Aseptic laboratory techniques: Plating methods. Journal of Visualized Experiments, 63, 3064.
  12. Slaoui, M., Bauchet, A. L., & Fiette, L. (2017). Tissue sampling and processing for histopathology evaluation. In Drug safety evaluation: Methods and protocols (pp. 101–114). Springer.
  13. Talaiekhozani, A. (2013). Guidelines for quick application of biochemical tests to identify unknown bacteria. Account of Biotechnology Research, 2(3), 1–15.
  14. Vishwas, K., Sandhyarani, K., Madhuri, D., Jeevanalatha, M., & Dhanalakshmi, K. (2000). Incidence and mortality due to respiratory diseases in rabbits. Journal of Entomology and Zoology Studies, 8, 706–711.
  15. Yershov, A. L., Jordan, B. S., Guymon, C. H., & Dubick, M. A. (2005). Relationship between the inoculum dose of Streptococcus pneumoniae and pneumonia onset in a rabbit model. European Respiratory Journal, 25(4), 693–700.

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