Biometry and Storage Ability at 4°C of Slaughtered Buck Testis and Correlations of Various Epididymal Seminal Attributes

The present study was conducted on 48 pairs of fresh testicles obtained from mature bucks, irrespective of breed, slaughtered at local
abattoir and AL-Nafees Proteins Pvt. Ltd., Mewat, Haryana. Various testicular and epididymal biometric parameters, epididymal seminal
attributes and correlation coefficients between testicular volume and seminal epididymal attributes were studied. The testicles with
epididymis were put randomly into six groups (plastic bags) each of eight pairs and were stored at 4°C for 96 hours in a refrigerator. One
bag/group each was taken out at 6, 12, 24, 48, 72, and 96 hours of cold storage to study the effect of preservation at 4°C on the seminal
epididymal attributes. The sperm concentration, total sperm output, progressive sperm motility, viability, HOS reactivity, and sperm
with droplets decreased significantly (p <0.05) and progressively increased sperm head and tail abnormalities after 12 hrs of storage
until 96 h. The testicular volume was significantly and positively correlated with sperm concentration (r = 0.76), total sperm output (r =
0.95), initial motility (r = 0.65), live sperm (r = 0.63), HOS reactive sperm (r = 0.66) and sperm with abnormal droplets (r = 0.60), however,
it was negatively correlated with abnormal sperm head (r = -0.64) and sperm tail (r = -0.69).