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Home▶ Journals▶ Indian Journal of Veterinary Sciences and Biotechnology▶Archive▶Vol. 17 No. 4 (2021)▶Molecular Detection and Sequencing of Peste Des Petits…
Indian Journal of Veterinary Sciences and Biotechnology
Indian Journal of Veterinary Sciences and Biotechnology ›
Print ISSN 2394-0247 · Online ISSN 2395-1176
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Molecular Detection and Sequencing of Peste Des Petits Ruminants Virus of Saurashtra Region of Gujarat, India

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Mahesh M. Tajpara — Krishi Vigyan Kendra, Junagadh Agricultural University, Junagadh, India,
Nalinkant M. Shah — Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, India
Bhaveshkumar B. Javia — Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, India
Dilipsinh B. Barad — Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, India
Umed V. Ramani — Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, India
Dharmesh R. Patel — Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, India
Vinay A. Kalaria — Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, India
Published 10 Oct 2021 Vol. 17 No. 4 (2021), pp. 17-22 ● Open access
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Abstract

The present study was undertaken with the objective of molecular detection and sequencing of the Saurashtra region’s Peste Des Petits  Ruminants virus. A total of 119 clinical samples (40 nasal swabs, 20 conjunctiva swabs, 16 oral swabs, and 19 tissues from goats along  with 22 nasal swabs and 2 tissues of sheep lung and intestine) were tested by N gene & F gene-based primers. Out of 119 samples,  35 samples were positive for N gene primer, and 32 positives for F gene primer. The N gene-based sequence analysis of three PPRV  obtained from Bhavnagar, Amreli, and Rajkot belonged to lineage IV (Indian strains), and all three PPRV sequences showed 100 %  genetic identity with each other and 98-99 % genetic identity with PPRV strain of Mehsana (North Gujarat), Bhopal (Madhya Pradesh),  Pune (Maharashtra), Jhansi (Uttar Pradesh), and Sungri (vaccine strain)  

KeywordsMolecular detectionPeste Des Petits Ruminants virus(PPR virus)SequencingSaurashtra regionGujarat

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