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Home▶ Journals▶ Indian Journal of Veterinary Sciences and Biotechnology▶Archive▶Vol. 17 No. 3 (2021)▶Isolation and Molecular Characterization of…
Indian Journal of Veterinary Sciences and Biotechnology
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Isolation and Molecular Characterization of Methicillinresistant Staphylococcus aureus (MRSA) from Swine following Spa Typing

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Indhuja Ganesan — Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education & Research, Kurumbapet, Pondicherry - 605 009, India
Prabhaker X. Antony — Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education & Research, Kurumbapet, Pondicherry - 605 009, India
Jayalakshmi Vasu — Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education & Research, Kurumbapet, Pondicherry - 605 009, India
Mouttou V. Srinivas — Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education & Research, Kurumbapet, Pondicherry - 605 009, India
Hirak K. Mukhopadhyay — Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education & Research, Kurumbapet, Pondicherry - 605 009, India
Jacob Thanislass — Department of Veterinary Biochemistry, Rajiv Gandhi Institute of Veterinary Education & Research, Kurumbapet, Pondicherry - 605 009, India
Published 16 Aug 2021 Vol. 17 No. 3 (2021), pp. 73-77 ● Open access
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Abstract

The objective of this study was to isolate the Methicillin-resistant Staphylococcus aureus (MRSA) from the nasal swabs of swine and
further characterize the isolates by spa typing. 116 (91.34%) Staphylococcal isolates were obtained from 127 nasal swabs of swine
from organized and unorganized pig farms in and around the Puducherry region (India). The nuc gene specific for S. aureus was
found in 97 (83.62%) isolates from swine. Methicillin resistant gene mecA was detected in 23 isolates (19.83%), and none of the
staphylococcal isolates from swine carried the mecC gene. Ten representatives MRSA isolates from swine were subjected to further
characterization using spa typing (Staphylococcus aureus protein A typing). Four different spa types were identified namely t657
(40.0%, 4/10), t5983 (40.0%, 4/10), t005 (10.0%, 1/10) and t127 (10.0%, 1/10) from Puducherry region.

KeywordsMethicillin-resistancemecAPublic health, Spa typingSwine

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