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Glutathione as a Semen Additive to Improve Surti Buffalo (Bubalus bubalis) Bull Semen Cryopreservation

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Kailash Kumar — Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334001, India
Mitesh Gaur — Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334001, India
Published 10 Jan 2022 Vol. 18 No. 1 (2022), pp. 116-118 ● Open access
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Abstract

The present study evaluated the effects of glutathione as an additive in Tris fructose egg yolk citrate extender on Surti buffalo bull semen.  Extended semen @ 80 million sperm/mL was divided into 4 equal aliquots and glutathione was added at 0.5 mM (T1), 2.0 mM (T2), and  5.0 mM (T3) with one control (T0) group. The percentages of post-thaw sperm progressive motility, live sperm, and HOS responsive  sperm were significantly higher (p <0.05), whereas sperm abnormalities were significantly lower (p <0.05) in treatment T1, T2 and T3  compared to T0 control. The treatment T2 (2.0 mM glutathione) had the highest post-thaw sperm progressive motility, live sperm, HOS  response sperm percentage and reduced sperm abnormalities compared to other three groups. It was concluded that glutathione at  2.0 mM concentration significantly (p <0.05) improved post-thaw semen quality of buffalo bulls in comparison to control and glutathione  concentrations of 0.5 mM and 5.0 mM in Tris extender. 

KeywordsCryopreservationGlutathionePost-thaw qualitySurti buffaloSemen additive

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