Abstract
Aflatoxin M1 (AFM1) is a hydroxylated metabolite of aflatoxin B1 from a contaminated feed. Aflatoxins are toxic, mutagenic and
carcinogenic compounds produced as a secondary metabolite by the fungi Aspergillus flavus and A. parasiticus. The present study was
conducted to determine the prevalence of AFM1 in milk by using two different diagnostic methods, viz., Enzyme-Linked Immunosorbent
Assay (ELISA) and Lateral Flow Assay (LFA). A total of 200 milk samples were collected from the areas under Junagadh city and nearby
villages. An overall 93.5% incidence rate of AFM1 contamination in milk samples was recorded. Out of 200 samples, 6 (3%) samples
were found to contain AFM1 above the FSSAI standard limit (0.5 µg/L) and 145 (72.5%) samples had AFM1 above the standard limit as
per EU legislation (0.05 µg/L). Out of total 200 samples, 50 representative samples ana
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