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Home▶ Journals▶ Indian Journal of Veterinary Sciences and Biotechnology▶Archive▶Vol. 21 No. 1 (2025)▶Comparative Genetic Characterization of Prolactin Gene…
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Research Article

Comparative Genetic Characterization of Prolactin Gene between Giriraja and Indigenous Chicken

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Ajeet Singh — Department of Animal Genetics and Breeding, Veterinary College, KVAFSU, Hebbal, Bengaluru-560 024, Karnataka, India
Naveen Kumar S — Department of Animal Genetics and Breeding, Veterinary College, KVAFSU, Hebbal, Bengaluru-560 024, Karnataka, India
Yathish H M — Department of Animal Genetics and Breeding, Veterinary College, KVAFSU, Hebbal, Bengaluru-560 024, Karnataka, India
Basavraj Inamdar — Department of Animal Genetics and Breeding, Veterinary College, KVAFSU, Hebbal, Bengaluru-560 024, Karnataka, India
Malathi V — Department of Poultry Science, Veterinary College, KVAFSU, Hebbal, Bengaluru-560 024, Karnataka, India
Published 22 Jan 2025 Vol. 21 No. 1 (2025), pp. 98-105 ● Open access
❝ How to cite 🔗 https://doi.org/10.48165/ijvsbt.21.1.20
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Abstract

The present study was conducted to determine the polymorphism and to explore the possible SNPs in prolactin gene among the Giriraja and Indigenous chicken using PCR-RFLP. About 80 birds each of Giriraja and Indigenous chicken were genotyped for promoter regions 1 and 2 (C-2402T and C- 2161G) of prolactin gene by employing published primers. The restriction enzymes, AluI and Csp6I were utilized for genotyping polymorphisms of C-2402T and C-2161G, respectively. At promoter region 1 (24 bp indel at -358) of prolactin gene, the proportions of DD and ID genotypes were higher in Giriraja (0.45) and Indigenous (0.44) chicken. However, the frequency of D allele (0.775 and 0.631) was higher in both the studied populations. Whereas, for promoter region 2 of prolactin gene, the frequencies of T allele (C-2402T) and G allele (C-2161G) were higher in Giriraja (0.806 and 0.694) and Indigenous (0.644 and 0.525) confirming that T alleles are predominant in native or indigenous chicken. Further, three SNPs, viz., C>T transition at 161 bp, T>C transition at 371 bp and C>G transversion at 402 bp were detected at promoter region 2 (C-2402T and C-2161G) in T/G allele compared to C allele.
KeywordsBroodinessGirirajaIndigenous chickenProlactin genePromoter

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