Effect of cutting size, cutting position, and Indole-3-Butyric Acid (IBA) on rooting and growth of Dalbergia sissoo cuttings
DOI:
https://doi.org/10.48165/ija.2026.28.02.13Keywords:
Dalbergia, macropropagation, cutting size, cutting position, IBA, mist chamberAbstract
In the present study, the effect of cutting size, position and different levels of growth hormone on the macropropagation and growth of plantlets in Dalbergia sissoo were evaluated. Clone 5 of Dalbergia sissoo from the clonal hedge orchard maintained at Research Farm of the Department of Forestry and Natural Resources, Punjab Agricultural University, Ludhiana was selected due to its vigorous growth, healthy juvenile shoots and superior rooting potential making it suitable for preparing the cuttings to examine the effect of cutting size, position and different levels of IBA treatment and changes in associated physiological traits during adventitious root formation. The vermiculite filled in trays (90 cc) were used as rooting medium. The cuttings of different sizes (4 cm, 7 cm and 10 cm) were treated with 4 hormonal treatments (0 ppm, 1000 ppm, 2000 ppm and 3000 ppm IBA) and were planted in the mist chamber conditions. The significant differences were found among cutting sizes and IBA levels for rooting and growth traits. The 7 cm cutting size showed the maximum rooting percentage (70.50 %) which was statistically at par with 4 cm cutting size (70 %) and 2000 ppm had the highest rooting percentage (74.45 %) which was statistically similar to 1000 ppm IBA level (73.33 %). The cuttings from different positions (top, middle and basal) were treated with 3 hormonal treatments (0 ppm, 1000 ppm and 2000 ppm IBA) and were planted in the mist chamber conditions. Cuttings taken from middle portion had maximum rooting (66.67 %). With regard to IBA levels, maximum rooting was observed at 2000 ppm treatment (66.67 %) which was statistically similar to 1000 ppm IBA level (64.17 %). A significant increase in all the metabolic activities was noted due to IBA. Total soluble sugars, starch content and total carbohydrates declined over time in all cuttings. For such decrease it has been suggested that hydrolytic enzymes release into the rooting zone of cuttings stored carbohydrates, primarily starch and sugars, where they are used to supply the energy required for cellular differentiation and division. The current research also revealed that stored carbohydrates were used in shoot cuttings during adventitious root development. Nitrogen content started to increase in the early stage and reached highest level on day 55 of planting, followed by decline at 80th day of planting. Overall these findings suggested that exogenous application of IBA may have activated carbohydrate metabolism.
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