Enhancing skin regeneration in gamma irradiated mice by Low-level laser Therapy
DOI:
https://doi.org/10.48165/Keywords:
Gamma-irradiation, mice, skin, He-Ne laserAbstract
Background: The skin is considered to be a radiosensitive organ. Low-level laser therapy (LLLT) known as photobiomodulation is a growing technology used to treat multitude of conditions that require stimulation of the healing process. Aim of the work: The present work was conducted to study the possible biostimulatory effects of LLLT on the skin of gamma irradiated mice. Materials and methods: 264female mice were used in the present experiments were divided into 6 groups: Group (1): Non irradiated control group. Group (2): Laser irradiated group. Group (3.1): One shot gamma irradiated group. Group (3.2): One shot + laser irradiated group. Group (4.1): Cumulative gamma irradiated group. Group (4.2): Cumulative + laser irradiated group. Exposure of the animals to gamma rays was performed using a cesium-137 source. Two modes of exposure were used, a shot modality where the mice were irradiated with a single sub lethal dose of 5 Gy. While in the other mode, the same dose was given in fractionated daily installations of 1 Gy. Treatment of mice with laser was carried out using a computerized scanner emitting He-Ne (CW) at a wavelength of 632.8 nm. The fluence was 5J/cm2. Histopathological (HP) assessment of skin biopsies derived from the experimental mice was done immediately after each sacrification.Conclusion: LLL therapy accelerates skin regeneration post exposure to ionizing radiation in both shot and cumulative mode. Our conclusions also highlight the biostimulatory effect of He-Ne LLL on hair follicles.
References
Alves, A.N.; Fernandes, K.P.; Deana, A.M.; Bussadori, S.K. and Mesquita-Ferrari, R.A. (2014): Effects of low-level laser therapy on skeletal muscle repair: A systematic review. Am. J. Phys. Med. Rehabil., 93(12): 1073.
Andrade, F.; Clark, R.M. and Ferreira, M.L. (2014): Effects of low-level laser therapy on wound healing. Rev. Col. Bras. Cir., 41(2): 129.
Avram, M.R. and Rogers, N.E. (2009): The use of low-level light for hair growth (Part 1). J. Cosmet. Laser Ther., 11(2): 110.
Avram, M.R.; Leonard, R.T.; Epstein, J.S.; Williams, J.L. and Bauman, A.J. (2007): The current role of laser/light sources in the treatment of male and female pattern hair loss. J. Cosmet. Laser Ther., 9(1): 27.
Bancroft, J.D. and Gamble, M. (2002): Bancroft’s Theory and Practice of Histological Techniques (5th ed.). Churchill Livingstone, London, New York & Sydney.
Chaves, M.E.; de Araújo, A.R.; Piancastelli, A.C. and Marcos, P.M. (2014): Effects of low-power light therapy on wound healing: LASER × LED. An. Bras. Dermatol., 89(4): 616.
Chung, H.; Dai, T.; Sharma, S.K.; Huang, Y.; Carroll, J.D. and Hamblin, M.R. (2012): The nuts and bolts of low-level laser (light) therapy. Ann. Biomed. Eng., 40(2): 516.
Di Lima, F.J.; de Oliveira Neto, O.B.; Barbosa, F.T.; do Nascimento-Galvão, A.M.; Ramos, F.W.; de Lima, C.C. and de Sousa Rodrigues, C.F. (2016): Is there a protocol in experimental skin wounds in rats using low-level diode laser therapy (LLDLT), combining or not red and infrared wavelengths? Lasers Med. Sci., 31(4): 779.
El Batanouny, M. (2009): Low-level laser therapy for wound healing: Clinical and biochemical assessments. In Z. Simunovic (Ed.), Laser in medicine science and practice in medicine, surgery, dentistry and veterinary, pp. 722–741.
El Batanouny, M.H.; Yousri, R.M.; Mahfouz, S. and Salem, E.S. (2019): Role of low-level laser in ameliorating the damaging effects of gamma irradiation on mice liver. Int. J. Radiat. Res., 17(1): 97.
Farivar, S. (2014): Biological effects of low-level laser therapy. J. Lasers Med., 5(2): 58.
Goodman, T.R. (2010): Ionizing radiation effects and their risk to humans. Yale University School of Medicine, New Haven, CT; American College of Radiology.
Hawkins, D.; Houreld, N. and Abrahamse, H. (2005): Low-level laser therapy (LLLT) as an effective therapeutic modality for delayed wound healing. Ann. N.Y. Acad. Sci., 1056: 486.
Hengel, S.M.; Aldrich, J.T.; Waters, K.M. and Stenoien, D.L. (2014): Quantitative proteomic profiling of low-dose ionizing radiation effects in a human skin model. Proteomes, 2(3): 382.
Ibe, O.; Morency, E.E.; Sosa, P. and Burkow-Heikkinen, L. (2015): The role of near-infrared light-emitting diodes in aging adults related to inflammation. Healthy Aging Res., 4: 24.
Isoir, M.; Buard, V.; Gasser, P.; Lati, E. and Benederitter, M. (2005): Human keratinocyte radioactivity is linked to redox modulation. J. Dermatol. Sci., 41(1): 55.
Kinoshita, K.; Ishimine, H.; Shiraishi, K.; Kato, H.; Doi, K.; Kuno, S.; Kanayama, K.; Mineda, K.; Mashiko, T.; Feng, J.; Nakagawa, K.; Kurisaki, A.; Itami, S. and Yoshimura, K. (2014): Cell and tissue damage after skin exposure to ionizing radiation: Short- and long-term effects after single and fractional doses. Cells Tissues Organs, 200(3–4): 240.
Lanzafame, R.J.; Blanche, R.R.; Chiacchierini, R.P.; Kazmirek, E.R. and Sklar, J.A. (2014): The growth of human scalp hair in females using visible red light laser and LED sources. Lasers Surg. Med., 46(8): 601.
Melo, V.A.; Anjos, D.C.; Albuquerque Júnior, R.; Melo, D.B. and Carvalho, F.U. (2011): Effect of low-level laser on sutured wound healing in rats. Acta Cir. Bras., 26(2): 129.
Merrifield, M. and Kovalchuk, O. (2013): Epigenetics in radiation biology: A new research frontier. Front. Genet., 4: 40.
Ng, G.Y.; Fung, D.T.; Leung, M.C. and Guo, X. (2004): Comparison of single and multiple applications of GaAlAs laser on rat medial collateral ligament repair. Lasers Surg. Med., 34(3): 285.
Piva, J.A.; Abreu, E.M.; Silva, V. and Nicolau, R.A. (2011): Effect of low-level laser therapy on the initial stages of tissue repair: Basic principles. An. Bras. Dermatol., 86(5): 947.
Poeggeler, B.; Bodó, E.; Nadrowitz, R.; Dunst, J. and Paus, R. (2010): A simple assay for the study of human hair follicle damage induced by ionizing irradiation. Exp. Dermatol., 19(8): e306.
Rashidi, S.; Yadollahpour, A. and Mirzaiyan, M. (2015): Considerations. Biomed. Pharmacol. J., 8(2): 1121.
Rezvani, M.; Rass, G.A. and Wilkinson, J.M. (2002): Evidence for humoral effect on radiation response of rat foot skin. Br. J. Radiol., 75(889): 50.
Singh, M.; Alavi, A.; Wong, R. and Akita, S. (2016): Radiodermatitis: A review of our current understanding. Am. J. Clin. Dermatol., 17(3): 277.
Wong, S.; Han Hor Chor, H.H.; Moorthy, S.; Ong, C.T.; Phan, T.T. and Lu, J.J. (2013): Human epidermal keratinocyte death and expression of protein markers of apoptosis after ionizing radiation exposure. Int. J. Cancer Ther. Oncol., 1(2): 0102.
Wu, P.S.; Fan, S.M.; Chiu, H.Y.; Tsai, T.H. and Lin, S.J. (2015): Enhancing hair follicle regeneration by nonablative fractional laser: Assessment of irradiation parameters and tissue response. Lasers Surg. Med., 47(4): 331.
Wynn, T.A. and Vannella, K.M. (2016): Macrophages in tissue repair, regeneration, and fibrosis. Immunity, 44(3): 450.
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