Biocontrol Potential of some Entomopathogenic Fungi against The Cotton Leaf Worm Spodoptera littoralis in vitro
DOI:
https://doi.org/10.48165/Keywords:
Entomopathogenic Fungi, Spodoptera Littoralis, Biocontrol, Czapek›s Agar, Protease Enzyme, Gamma IrradiationAbstract
The objective of this study was to isolate and identify the entomopathogenic fungi as biocontrol agents and to evaluate their pathogenicity against the cotton leaf worm, Spodoptera littoralis (Boisd.). Six strains of fungi were isolated from healthy and infected plants using dilution plate method. Czapek’s agar supplemented with 0.5 % yeast extract and potato dextrose agar, amended with rose bengal (1/15000) and chloramphenicol (50 ppm) was used for primary isolation. Aspergillus tamarii, Aspergillus parasiticus, pencillium sp., Trichoderma harzianum,Cladosporium sp .and Rhizopus sp. were isolated . Results showed that A. parasiticus , A. tamarii, T. harzianum and Pencillium sp. are the most promonent so in this study we test their virulence against third instar larvae of the cotton leaf worm the mortality percentage were calculated for each fungus using 1×106, 1×107 and 1×108 spore per ml. It is clear from the results that the mortality percentage increased with elapsing time. The high mortality percentage recorded is 86.66% for A.tamarii at 1×108 spore/ ml then 83.33% with A.parasiticus at 1×107 spore/ml, 80% for T. harizianum at 1×106 spore/ml and 40% at 1×107spore/ml for Pencillium sp. Comparison to these fungi clear that concentration 107 give the highest mortality percentage to the cotton leaf worm for all except A.tamarii .Then screening to the ability of these fungi to produce protease enzyme reveals that all of them produce protease enzyme except Pencillium sp. Gamma irradiation was used to enhance the protease enzyme activity and consequently increase the mortality percentageThe dose level of 1200 Gy increase protease activity of A. tamarii from 0.433 to 1.366 so mortality percentage of third instar larvae of Spodoptera littoralis increased from 43.33 % to 86.66 % at concentration 1×107 of spore suspension irradiated with 1200 Gy dose level..
References
Ahmed, A.M. and El-Katatny, M.H. (2007): Entomopathogenic fungi as bio pesticides against the Egyptian cotton leaf worm, Spodoptera littoralis: Between biocontrol promise and immune-limitation. J. Egypt. Soc. Toxicol., 37: 39.
Al-Aidoors, K. and Roberts, D.W. (1978): Mutants of Metarhizium anisopliae with increased virulence toward mosquito larvae. Can. J. Gen. Cyt., 11: 211.
Amer, M.M.; El-Sayed, T.I.; Bakheit, H.K.; Moustafa, S.A. and El-Sayed, Y.A. (2008): Pathogenicity and genetic variability of five entomopathogenic fungi against Spodoptera littoralis. Res. J. Agri. Sci., 4(5): 354.
Amin, N.; Daha, L. and Agus, N. (2014): The study on the role of entomopathogenic fungal endophytes in controlling the Pod Borer (Conopomorpha cramerella (Snellen)) (Lepidoptera: Gracillariidea) on cocoa plant. J. Entomology, 11(3): 142.
Anagnostakis (1975): Use of solid media for detection of enzyme production by fungi. Mycologia, 597.
Anand, R. and Tiwary, B.N. (2009): Pathogenicity of entomopathogenic fungi to eggs and larvae of Spodoptera litura, the common cutworm. Biocon. Sci. Tech., 19(9): 19.
Anand, R.; Prasad, B. and Tiwary, B.N. (2008): Relative susceptibility of Spodoptera litura pupae to selected entomopathogenic fungi. Biocontrol, 54(1): 85.
Ansari, M.A.; Vestergaard, S.; Tirry, L. and Moensa, M. (2004): Selection of a highly virulent fungal isolate, Metarhizium anisopliae CLO 53, for controlling Hoplia philanthus. J. Invertebr. Pathol., 85(2): 89.
Arx, J.V.; Guarro, J. and Figueras, M.J. (1986): The ascomycete genus Chaetomium. Nova Hedwigia Beihefte, 84(1): 162.
Arx, J.V. (1981): The genera of fungi sporulating in pure culture (3rd ed.). J. Cramer, Vaduz. 424.
Asi, M.R.; Bashir, M.H.; Afzal, M. and Imran, S. (2009): Effect of conidial concentration of entomopathogenic fungi on mortality of cabbage aphid, Brevicoryne brassicae L. Pak. J. Life. Soc. Sci., 2: 175.
Bin, W. and Mitsuaki, S. (2006): Density dynamics of an entomopathogenic fungus, Beauveria bassiana, introduced into fresh water. Bulletin of FFPRI, 5: 227.
Boucias, D.G. and Pendland, J.C. (1982): Ultrastructural studies on the fungus, Nomuraea rileyi, infecting the velvet bean caterpillar, Anticarsia gemmatalis. J. Invertebr. Pathol., 39: 338.
Boucias, D.G.; Farmerie, W.G. and Pendland, J.C. (1998): Cloning and sequencing the cDNA of the insecticidal toxin, Hirsutellin A. J. Invertebr. Pathol., 72: 258.
Boucias, D.G.; Farmerie, W.G. and Pendland, J.C. (1998): Cloning and sequencing the cDNA of the insecticidal toxin, Hirsutellin A. J. Invertebr. Pathol., 72: 258.
Casarelett, A.P. (1968): Radiation biology. United States Atomic Energy Commission, Washington, D.C., USA.
Castellanos–Moguel, J.; Cruz-Camarillo, R.; Aranda, E.; Mier, T. and Toriello, C. (2008): Relationship between protease and chitinase activity and the virulence of Paecilomyces fumosoroseus in Trialeurodes vaporariorum (Hemiptera: Aleyrodidae). Rev. Mex. Mico., 28: 73.
Charnley, A.K. (1984): Physiological aspects of destructive pathogenesis in insects by fungi: A speculative review. In Invertebrate-Microbial Interactions. British Mycological Society Symposium 6, Cambridge University Press, London, pp. 229.
Claydon, N.; Hanson, J.R.; Truneh, A. and Avent, A.G. (1991): Harzianolide, a butenolide metabolite from cultures of Trichoderma harzianum. Phy. Chem., 30: 3802.
Dias, B.A.; Neves, P.M.; Furlaneto-Maia, L. and Furlaneto, M.C. (2008): Cuticle degrading proteases produced by the entomopathogenic fungus Beauveria bassiana in the presence of coffee berry borer cuticle. Braz. Micr., 39: 301.
Dickinson, J.M.; Hanson, J.R. and Trunch, A. (1995): A metabolites of some biological control agents. Pest. Sci., 44: 389.
Domsch, K.H.; Gams, W. and Anderson, T.H. (1980): Compendium of soil fungi. Academic Press, London, England. 865 p.
Elliot, S.L.; Sabelis, M.W. and Janssen, A. (2000): Can plants use entomopathogens as bodyguards? Ecol. Lett., 3: 228.
Ellis, M.B. (1971): Dematiaceous Hyphomycetes. The Commonwealth Mycological Institute, England, pp. 1.
Ellis, M.B. (1976): More Dematiaceous Hyphomycetes. The Commonwealth Mycological Institute, England, pp. 1.
Evans, H.C. (1999): Biological control of weed and insect pests using fungal pathogens. Biocon. News & Inform., 20(2): 63.
Feng, M.G. (1998): Reliability of extracellular protease and lipase activities of Beauveria bassiana isolates used as their virulence indices. Acta. Micr. Sini., 38: 461.
Ferron, P. (1978): Biological control of insect pests by entomopathogenic fungi. Ann. Rev. Entom., 23: 409.
Ferron, P. (1981): Pest control by the fungi Beauveria and Metarhizium. In Microbiol Control of Pest Plant Diseases, H.D. Burges (Ed.), p. 465.
Gherbawy, A.M.H. (1998): Effect of gamma irradiation on the production of cell wall degrading enzymes by Aspergillus niger. Int. J. Foo. Mic. Bio., 40: 127.
Gillespie, J.P.; Bateman, R. and Charnley, A.K. (1998): Role of cuticle-degrading protease in the virulence of Metarhizium spp. for the desert locust, Schistocerca gregaria. J. Invertebr. Pathol., 71: 128.
Haggag, W.M. and Mohmed, H.A.A. (2002): Enhancement of antifungal metabolites production from gamma-ray induced mutants of some Trichoderma species for control of onion white rot disease. Plan. Patho. Bull., 11: 45.
Hajek, A.E. and St. Leger, R.J. (1994): Interactions between fungal pathogens and insect hosts. Ann. Rev. Entom., 39: 293.
Hassan, A.E.M.; Dillion, R.J. and Charnley, A.K. (1989): Influence of accelerated germination of conidia on the pathogenicity of Metarhizium anisopliae for Manduca sexta. J. Invertebr. Pathol., 54: 227.
Hicks, B.J.; Watt, A.D. and Cosens, D. (2001): The potential of Beauveria bassiana (Hyphomycetes: Moniliales) as a biological control agent against the pine beauty moth, Panolisammea (Lepidoptera: Noctuidae). Fore. Eco. & Manage., 149: 275.
Hidalgo, E.; Moore, D. and Le Patourel, G. (1998): The effect of different formulations of Beauveria bassiana on Sitophilus zeamais in stored maize. J. Stor. Prod. Res., 34: 171.
Hoell, I.A.; Klemsdal, S.S.; Vaaje-Kolstad, G.; Horn, S.J. and Eijsink, V.G.H. (2005): Overexpression and characterization of a novel chitinase from Trichoderma atroviride strain P1. Bio. Chim. & Bio. Phys. Acta., 1748: 180.
Ismail, M. and Abdel-Sater, M.A. (1993): Fungi associated with the Egyptian cotton leaf worm Spodoptera littoralis Boisdoval. Mycopathologia, 124(2): 79.
Johnson, L.F.; Curl, E.A.; Bond, J.H. and Fribourg, H.A. (1960): Method for studying soil microflora-plant disease relationship. Burgess Publishing Co., Minneapolis, USA.
Jones, H.E.; West, H.M.; Chamberlain, P.M.; Parekh, N.R.; Beresford, N.A. and Crout, N.M.J. (2004): Effects of gamma irradiation on Holcus lanatus (Yorkshire fog grass) and associated soil microorganisms. J. Envir. Rad. Acti., 74: 57.
Kirk, P.M.; Cannon, P.F.; David, J.C. and Stalpers, J.A. (2001): Ainsworth & Bisby’s dictionary of the fungi (9th ed.). CABI Publishing, Wallingford.
Lacey, L.A.; Frutos, R.; Kaya, H.K. and Vails, P. (2001): Insect pathogens as biological control agents: Do they have future? Biocontrol. J., 21(3): 230.
Leatherdale, D. (1970): The arthropod hosts of entomopathogenic fungi in Britain. Entomophaga, 15: 419.
Lin, H.P.; Yang, X.J.; Gao, Y.B. and Li, S.G. (2007): Pathogenicity of several fungal species on Spodoptera litura. Chinse J. Appl. Eco., 18(4): 937.
Malikarjuna, N.; Kranthi, K.R.; Jadhav, D.R.; Kranthi, S. and Chandra, S. (2004): Influence of foliar chemical compounds on the development of Spodoptera litura (Fab.) in inter-specific derivatives of groundnut. J. Appl. Entom., 128: 321.
Mustafa, U. and Kaur, G. (2009): Extracellular enzyme production in Metarhizium anisopliae isolates. Folia. Mic., 54(6): 499.
Nguyen, N.C.; Borgemeister, H.P. and Zimmermann, G. (2007): Laboratory investigations on the potential of entomopathogenic fungi for biocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae) larvae and pupae. Biocon. Sci. Tec., 17(12): 853.
Olive, P. (1998): The role of DNA single and double-strand breaks in cell killing by ionizing radiation. Rad. Res., 150: 42.
Palomares, J.; Palenius, H.; Avalos, M.; Rangel, A. and Gutiérrez, R. (2014): Occurrence of entomopathogenic fungus from flea Ctenocephalides canis (Siphonaptera: Pulicidae). J. Veter. Med., 4: 281.
Pitt, J.I. (1980): The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces. Academic Press, London. 634 p.
Purwar, J.P. and Sachan, G.C. (2005): Biotoxicity of Beauveria bassiana and Metarhizium anisopliae against Spodoptera litura and Spilarctia oblique. Ann. Plan. Prot. Sci., 13(2): 360.
Raper, K.B. and Thom, C. (1949): A manual of the Penicillia. Williams & Wilkins Company, Baltimore. 875 p.
Roberts, D.W. and Humber, R.A. (1981): Entomogenous fungi. In Cole, G.T. and Kendrick, B. (Eds.), Biology of Conidial Fungi. Academic Press, New York, p. 201.
Saikkonen, K.; Wali, P.; Helander, M. and Faeth, S.H. (2004): Evolution of endophyte plant symbioses. Tren. Plan. Sci., 9: 275.
Samson, R.A.; Evans, H.C. and Latge, J.P. (1988): Atlas of entomopathogenic fungi. Springer Verlag, Berlin, Germany, pp. 299.
Samuels, K.D.Z.; Heale, J.B. and Liewellyn, M. (1989): Characteristics relating to the pathogenicity of Metarhizium anisopliae toward Niaparvata lugens. J. Invertebr. Pathol., 53: 25.
Shahid, A.A.; Qayyumrao, A.; Bakhash and Husnain (2012): Entomopathogenic fungi as biological controllers: New insights into their virulence and pathogenicity. Arch. Bio. Sci., Belgrade, 64(1): 21.
Shakeri, J. and Foster, H.A. (2007): Proteolytic activity and antibiotic production by Trichoderma harzianum in relation to pathogenicity to insects. Enz. & Mic. Tech., 40: 961.
Shoulkamy, M.A.; Salama, S.A. and Ahmad, S.M. (2014): Use of entomopathogenic fungi for control of the cotton leaf worm, Spodoptera litoralis. J. Jazan Univ. Appl. Sci. Bran., 3(1): 1.
Smith, H.H. (1958): Radiation in the production of useful mutations. Bot. Rev., 24: 1.
Smith, R.J.; Pekrul, S. and Grula, E.A. (1981): Requirement for sequential enzymatic activities for penetration of the integument of the corn earworm. J. Invertebr. Pathol., 38: 335.
Smith, S.M.; Moore, D.; Karanja, L.W. and Chandi, E.A. (1999): Formulation of vegetable fat pellets with pheromone and Beauveria bassiana to control the larger grain borer, Prostephanus truncates (Horn). Pest. Sci., 55: 711.
St. Leger, R.J.; Durrands, P.K.; Chamely, A.K. and Cooper, R.M. (1988): Role of extracellular chymoelastase in the virulence of Metarhizium anisopliae for Manduca sexta. J. Invertebr. Pathol., 52: 285.
St-Leger, R.J.; Charnley, A.K. and Cooper, R.M. (1987): Characterization of cuticle degrading proteases produced by the entomopathogen Metarhizium anisopliae. Arch. Biochem. & Biophys., 253: 221.
St-Leger, R.J.; Joshi, L.; Bidochka, M.J.; Rizzo, N. and Roberts, D.W. (1996): Biochemical characterization and ultrastructural localization of two extracellular trypsins produced by Metarhizium anisopliae in infected insect cuticles. Appl. & Envir. Micr., 62: 1257.
Thom, C. and Raper, K.B. (1945): Manual of the Aspergilli. Williams and Wilkins, Baltimore.
Ujian, A.A. and Shahzad, S. (2007): Pathogenicity of Metarhizium anisopliae, var. Acridum strains on pink hibiscus mealy bug (Maconellicoccus hirsutus) affecting cotton crop. Pakist. J. Bot., 39(3): 96.
Vargas, L.B.; Rossato, M.; Ribeiro, R.S. and de Barros, N.M. (2003): Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity. Braz. Arch. Bio & Tech., 46: 13.
White, J.F.; Belanger, F.; Meyer, W.; Sullivan, R.F.; Bischoff, J.F. and Lewis, E.A. (2002): Clavicipitalean fungal epibionts and endophytes development of symbiotic interactions with plants. Symbiosis, 33: 201.
Wraight, S.P.; Carruthers, R.I.; Bradley, C.A.; Jaronski, S.T.; Lacey, L.A.; Wood, P. and Galaini, W.S.Z. (1998): Pathogenicity of entomopathogenic fungi Paecilomyces spp. and Beauveria bassiana against silver whitefly, Bemisia argentifolii. J. Invertebr. Pathol., 71: 217.
Zacharuk, R.Y. (1973): Electron-microscope studies of the histopathology of fungal infections by Metarhizium anisopliae. Misc. Pub. Entom. Soc. Amer., 9: 112.
Zimmerman, G. (1993): The entomopathogenic fungus Metarhizium anisopliae and its potential as a biocontrol agent. Pest. Sci., 37: 375–379.
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