Dielectric Monitoring the Effect Green Copper Oxide Nanoparticles Incubation with Baker's Yeast Cells Suspension

Authors

  • Rasha Plant Research Department, Nuclear Research Center, Atomic Energy Authority, Inshas, 13759, Egypt. Author
  • S Shams El Saadani Plant Research Department, Nuclear Research Center, Atomic Energy Authority, Inshas, 13759, Egypt. Author
  • M H Moustafa Plant Research Department, Nuclear Research Center, Atomic Energy Authority, Inshas, 13759, Egypt. Author

DOI:

https://doi.org/10.21608/jntas.2020.32846.1022

Keywords:

dielectrics, green synthesis, copper oxide, nanoparticles, yeast

Abstract

The current era of emerging nano-technology has immersed us in a sea of nanomaterials used in different fields of life. Copper oxide nanoparticles (CuONPs) are used in many industrial and medical issues. many previous studies were conducted upon CuONPs cytotoxicity interaction upon the biological cells. The current monitoring interventions of cell viability was carried out by several methods mainly depends on cytolysis or membrane leakage. unfortunately many of these assays are invasive to cell and the others are toxic. on the other-side physical means interventions play a crucial role. Among Electrical impedance measurement was a rational tool to investigate the biological materials by the mean of two electrodes in a sophisticated designed measuring cell. The current attempt aims at dielectric monitoring in form permittivity and conductivity spectrum of green (CuONPs) incubation effect with baker's yeast cells suspension. CuONPs are characterized by electron microscopy and Fourier transformer infred(FTIR) was investigated under room condition.

References

Aslantürk, Ö. S. (2017). In vitro cytotoxicity and cell viability assays: Principles, advantages, and disadvantages. In M. L. Marcelo & S. Sonia (Eds.), Genotoxicity—A predictable risk to our actual world. IntechOpen.

Awwad, A. M., Salem, N. M., & Abdeen, A. O. (2013). Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity. International Journal of Industrial Chemistry, 4, 29.

Downey, B., Graham, L., Breit, J., & Glutting, N. (2014). A novel approach for using dielectric spectroscopy to predict viable cell volume (VCV) in early process development. Biotechnology Progress, 30(2), 479.

Galván Márquez, I., Ghiyasvand, M., Massarsky, A., Babu, M., Samanfar, B., Omidi, K., Moon, T., Smith, M., & Golshani, A. (2018). Zinc oxide and silver nanoparticles toxicity in the baker’s yeast, Saccharomyces cerevisiae. PLOS ONE, 13(3), 0193111.

Jagathesan, G., & Rajiv, P. (2018). Biosynthesis and characterization of iron oxide nanoparticles using Eichhornia crassipes leaf extract and assessing their antibacterial activity. Biocatalysis and Agricultural Biotechnology, 13, 90.

Ogawa, R., Baidillah, M., Akita, S., & Takei, M. (2020). Investigation of physiological swelling on conductivity distribution in lower leg subcutaneous tissue by electrical impedance tomography. Journal of Electrical Bioimpedance, 11(1), 19.

Roa Romero, L. (2013). Proceedings of the 2nd Mediterranean Conference on Medical and Biological Engineering and Computing.

Roohvand, F., Shokri, M., Abdollahpour-Alitappeh, M., & Ehsani, P. (2017). Biomedical applications of yeast: A patent view, part one: Yeasts as workhorses for the production of therapeutics and vaccines. Expert Opinion on Therapeutic Patents, 27(8), 929.

Rossetto, A., Melegari, S., Ouriques, L., & Matias, W. (2014). Comparative evaluation of acute and chronic toxicities of CuO nanoparticles and bulk using Daphnia magna and Vibrio fischeri. Science of the Total Environment, 490, 807.

Schwan, H. (1963). Electric characteristics of tissues. Biophysik, 1(3), 198.

Sharma, S., & Uttam, K. (2017). Rapid analyses of stress of copper oxide nanoparticles on wheat plants at an early stage by laser-induced fluorescence and attenuated total reflectance Fourier transform infrared spectroscopy. Vibrational Spectroscopy, 92, 135–150.

Wongrakpanich, A., Mudunkotuwa, I., Geary, S., Morris, A., Mapuskar, K., Spitz, D., Grassian, V., & Salem, A. (2016). Size-dependent cytotoxicity of copper oxide nanoparticles in lung epithelial cells. Environmental Science: Nano, 3(2), 365.

Yao, J., Liu, X., Xu, Z., Zhao, T., Chen, B., & Wu, H. (2019). Electrical impedance tomography for biological cell sensing with microfluidic device. Mechanical Engineering Journal, 55(2), 1.

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Published

2020-10-20

How to Cite

Dielectric Monitoring the Effect Green Copper Oxide Nanoparticles Incubation with Baker’s Yeast Cells Suspension. (2020). Journal of Nuclear Technology in Applied Science, 8(1), 119-123. https://doi.org/10.21608/jntas.2020.32846.1022