Efficacy of Ovsynch Protocol with Double Dose of GnRH in Postpartum Anestrus Mehsana Buffaloes
DOI:
https://doi.org/10.48165/ijvsbt.2026.22.06.36Keywords:
Anestrus, Buffalo, Conception rate, Estrus, GnRH, OvsynchAbstract
The present study compared the efficacy of the Ovsynch and modified Ovsynch protocols in enhancing fertility in anestrus postpartum Mehsana buffaloes. Sixteen buffaloes were selected and randomly divided into two groups: Group I and Group II. Both groups were treated with the Ovsynch protocol, with a modification in the dose of GnRH in Group II, which received a double dose of GnRH, 20 μg (5 mL) buserelin acetate, instead of 10 μg (2.5 mL), intramuscularly. The buffaloes underwent FTAI at 12 to 24 h post-second buserelin acetate injection. The rate of induced estrus was recorded as 100 % in both groups. The estrus duration and conception rate were higher in Group II than in Group I (21±1.96 vs 20.25±1.94 h; 87.50% vs 75.00%, respectively). It is concluded that the double dose of GnRH analogue used in the Ovsynch protocol improved estrus duration and conception rates in postpartum anestrus Mehsana buffaloes, with equal estrus induction efficacy.
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Agarwal, S. K., Umashankar, & Ansari, M. R. (2004). Laboratory manual on gynaecology. Communication Centre, IVRI.
Ali, A., & Fahmy, S. (2007). Ovarian dynamics and milk progesterone concentration in cycling and non-cycling buffalo cows (Bubalus bubalis) during Ovsynch program. Theriogenology, 68(1), 23–28.
Alyas, A., Razzaque, W. E. E., Ali, R., Rao, M. M., Kumar, S., Bharadwaj, H. R., & Hussain, K. (2013). Supplementation of progesterone in Ovsynch to improve fertility in postpartum anestrous buffaloes. International Journal of Advanced Research, 1(5), 79–82.
Berber, R. C. A., Madureira, E. H., & Baruselli, P. S. (2002). Comparison of two Ovsynch protocols (GnRH versus LH) for fixed timed insemination in buffalo (Bubalus bubalis). Theriogenology, 57(5), 1421–1430.
El-Wishy, A. B. (2007). The postpartum buffalo. II. Acyclicity and anestrus. Animal Reproduction Science, 97(3–4), 216–236.
Ghuman, S., Honparkhe, M., & Singh, J. (2014). Comparison of Ovsynch and progesterone-based protocol for induction of synchronized ovulation and conception rate in subestrous buffalo during low breeding season. Iranian Journal of Veterinary Research, 15(4), 375–378.
Kajaysri, J., Chumchoung, C., & Photikanit, G. (2015). Estrus and ovulation responses in anestrus postpartum swamp buffaloes following synchronization with a controlled internal drug release device and prostaglandin F₂α-based protocols. Buffalo Bulletin, 34(3), 357–368.
Kalaswa, S. H., Nakhashi, H. C., Suthar, B. N., Sutaria, T. V., Joshi, R. D., & Pateliya, H. R. (2017). Efficacy of hormonal and herbal therapy in postpartum true anoestrus Mehsana buffaloes. Trends in Biosciences, 10(31), 6452–6457.
Kumar, A., Sharma, U., Singh, R., Kumar, S., & Kumar, S. (2015). Changes in haemato-biochemical profile in postpartum anestrous Murrah buffalo subjected to different hormonal protocols. Indian Veterinary Journal, 92(5), 50–52.
Kose, M., & Tekeli, T. (2014). Effect of ovarian functions on estrus synchronization and pregnancy rate in Brown Swiss heifers and non-lactating cows. Eurasian Journal of Veterinary Sciences, 30(2), 53–58.
Malik, R. K., Singh, P., Singh, I. J., Sharma, R. K., Phulia, S. K., Tuli, R. K., & Chandolia, R. K. (2011). Ovarian response and fertility of Ovsynch-treated postpartum anestrus Murrah buffaloes. Buffalo Bulletin, 30(4), 272–276.
Nakrani, B. B., Panchal, M. T., Dhami, A. J., Hadiya, K. K., Patel, J. A., & Gosai, R. K. (2014). Effect of controlled breeding techniques on fertility and plasma profile of biochemical and mineral constituents in anoestrus buffaloes. Scholars Journal of Agriculture and Veterinary Sciences, 1(4B), 299–304.
Prasad, B. C., & Rao, A. K. (2014). Effect of PGF₂α, GnRH and Ovsynch in anestrous buffaloes at Buffalo Research Station in Andhra Pradesh. Indo-American Journal of Agricultural and Veterinary Science, 2(1), 16–18.
Pursley, J. R., Mee, M. O., & Wiltbank, M. C. (1995). Synchronization of ovulation in dairy cows using PGF₂α and GnRH. Theriogenology, 44, 915–923.
Pursley, J. R., Wiltbank, M. C., Stevenson, J. S., Ottobre, H., Garverick, H. A., & Anderson, L. L. (1997). Pregnancy rates in cows and heifers inseminated at a synchronized ovulation or synchronized estrus. Journal of Dairy Science, 80, 295–300.
Rathore, R., Sharma, R. K., Phulia, S. K., Mudgal, V., Jerome, A., & Ghuman, S. P. S. (2017). Comparative efficacy of oestrus synchronization protocols in buffalo (Bubalus bubalis). Tropical Animal Health and Production, 49, 1377–1382.
Raval, R., Parmar, K., Vala, K., Solanki, G., & Parikh, S. S. (2017). Comparative efficacy of different medicaments for induction of estrus in true anestrus Jafarabadi buffalo heifers. Indian Journal of Veterinary Sciences and Biotechnology, 12(4), 30–33.
Renesis, F. D., Ronchi, G., Guarneri, P., Nguyen, B. X., Presicce, G. A., Huszenicza, G., & Scaramuzzi, R. J. (2005). Efficacy of the Ovsynch protocol for synchronization of ovulation and fixed time artificial insemination in Murrah buffaloes (Bubalus bubalis). Theriogenology, 63(7), 1824–1831.
Savani, H. R., Khasatiya, C. T., Saxena, S., & Patel, M. D. (2017). Estrus induction and fertility response following Ovsynch alone and Ovsynch plus PRID protocol in postpartum anestrus Surti buffaloes. Indian Journal of Veterinary Sciences and Biotechnology, 12(4), 1–4.
Vijyeta, H. P., Solanki, G. B., Chaudhary, J. K., Gamit, P. M., & Parikh, S. S. (2017). Efficacy of GnRH–prostaglandin–GnRH schedule in management of postpartum anestrus and subestrus Jafarabadi buffaloes. Indian Journal of Veterinary Sciences and Biotechnology, 12(4), 11–13.
Yendraliza, Z., Udin, B. P., Jaswandi, Z., & Arman, C. (2011). Effect of combination of GnRH and PGF₂α for estrus synchronization on onset of estrus and pregnancy rate in different postpartum swamp buffaloes in Kampar Regency. Journal of the Indonesian Tropical Animal Agriculture, 36(1), 9–13.
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