Histopathological Analysis and Renal Toxicity of Silver Nanoparticles upon Frequent Oral Exposure in Wistar Rats
DOI:
https://doi.org/10.48165/ijvsbt.2026.22.06.11Keywords:
Immunohistochemistry, Histopathology, Renal toxicity, Silver nanoparticles, Wistar ratAbstract
Silver Nanoparticles (AgNPs) are the most widely produced engineered nanoparticles and account for more than 50% of global nanomaterial consumer products. Because of their small size, AgNPs can enter the human body through ingestion, inhalation, and skin contact. Kidneys, lungs, nervous system, and liver are organs prone to the accumulation of AgNPs and cause various histological changes as well as damage to organs. The objective of this study was to evaluate the renal toxicity induced by AgNPs after repeated oral exposure through pathomorphology and immunohistochemistry of kidneys in Wistar rats. Wistar rats (n=35) of either sex were randomly classified into two groups (treated, n=20; and control group, n=15). The control group of rats received normal feed and water, while test rats were given AgNPs in the form of suspension by oral gavaging at NOAEL dose (30 mg/kg b.wt.). On day 0, 5 rats from test group were weighed and euthanized, while at days 30th, 60th and 90th of the experiment, 5 rats from each control and test group were weighed and humanely euthanized to check pathomorphological changes of kidney. Histopathological changes were assessed using hematoxylin-eosin (H&E) stain. For immunohistochemistry, at day 90th kidney tissue sections of treated rats were examined for the presence of antigen-antibody complexes using immunoperoxidase reaction to check histopathological changes. Pathomorphological studies revealed gross changes after 90 days of AgNPs exposure as compared to control group. Congestion and endothelial swelling in glomerular capillaries, focal infiltration of inflammatory cells in the glomeruli and in medullary area, cloudy swelling of the kidney tubular epithelium (KTE) which later progressed to coagulative necrosis were seen. Apoptotic figures like blebs were seen in the renal tubules. Proliferation of mesangial cells and expansion of mesangial matrix was seen at 60th and 90th day of experiment. Thus, AgNPs were responsible for in vivo renal damage.
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Albrahim, T. (2020). Silver nanoparticles-induced nephrotoxicity in rats: The protective role of red beetroot (Beta vulgaris) juice. Environmental Science & Pollution Research, 27(31), 38871–38880.
Attia, H., Nounou, H., & Shalaby, M. (2018). Zinc oxide nanoparticles induced oxidative DNA damage, inflammation and apoptosis in rat’s brain after oral exposure. Toxics, 6(2), 29.
Chairuangkitti, P., Lawanprasert, S., Roytrakul, S., Aueviriyavit, S., Phummiratch, D., Kulthong, K., et al. (2013). Silver nanoparticles induce toxicity in A549 cells via ROS dependent and ROS-independent pathways. Toxicology in Vitro, 27(1), 330–338.
Choi, J. I., Chae, S. J., Kim, J. M., Choi, J. C., Park, S. J., Choi, H. J., & Park, H. J. (2018). Potential silver nanoparticles migration from commercially available polymeric baby products into food simulants. Food Additives & Contaminants: Part A, 35(5), 996–1005.
Crosera, M., Bovenzi, M., Maina, G., Adami, G., Zanette, C., Florio, C., & Filon Larese, F. (2009). Nanoparticle dermal absorption and toxicity: A review of the literature. International Archives of Occupational and Environmental Health, 82(9), 1043–1055.
Culling, C. F. A. (2013). Handbook of histopathological and histochemical techniques: Including museum techniques. Butterworth-Heinemann.
Gaillet, S., & Rouanet, J. M. (2015). Silver nanoparticles: Their potential toxic effects after oral exposure and underlying mechanisms—A review. Food and Chemical Toxicology, 77, 58–63.
Gambardella, C., Costa, E., Piazza, V., Fabbrocini, A., Magi, E., Faimali, M., & Garaventa, F. (2015). Effect of silver nanoparticles on marine organisms belonging to different trophic levels. Marine Environmental Research, 111, 41–49.
Gopinath, V., Priyadarshini, S., Loke, M. F., Arunkumar, J., Marsili, E., Mubarak Ali, D., & Vadivelu, J. (2017). Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity. Arabian Journal of Chemistry, 10(8), 1107–1117.
Hassanen, E. I., Khalaf, A. A., Tohamy, A. F., Mohammed, E. R., & Farroh, K. Y. (2019). Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats. International Journal of Nanomedicine, 14, 4723–4739.
Jafarzadeh, S. R., Heydarnejad, M. S., & Kabiri, S. M. (2015). A survey of acute histopathological effects of silver nanoparticles on liver, kidney with blood cells during oral administration in male mice (Mus musculus). Journal of Shahrekord University of Medical Sciences, 17(4), 97–107.
Jeevanandam, J., Barhoum, A., Chan, Y. S., Dufresne, A., & Danquah, M. K. (2018). Review on nanoparticles and nanostructured materials: History, sources, toxicity and regulations. Beilstein Journal of Nanotechnology, 9(1), 1050–1074.
Joshi, A., & Chauhan, R. S. (2012). Immunological techniques: Interpretations, validation and safety measures. Kapish Prakashan.
Khurana, R., & Chauhan, R. S. (2002). Pesticide induced immune complex mediated glomerulonephritis in sheep. Journal of Immunology and Immunopathology, 4(1–2), 40–42.
Kim, Y. S., Song, M. Y., Park, J. D., Song, K. S., Ryu, H. R., Chung, Y. H., & Yu, I. J. (2010). Subchronic oral toxicity of silver nanoparticles. Particle and Fibre Toxicology, 7(1), 20.
Mackevica, A., Olsson, M. E., & Hansen, S. F. (2016). Silver nanoparticle release from commercially available plastic food containers into food simulants. Journal of Nanoparticle Research, 18(1), 5.
Mao, B. H., Chen, Z. Y., Wang, Y. J., & Yan, S. J. (2018). Silver nanoparticles have lethal and sublethal adverse effects on development and longevity by inducing ROS-mediated stress responses. Scientific Reports, 8(1), 2445.
Nosrati, H., Hamzepoor, M., Sohrabi, M., Saidijam, M., Assari, M. J., Shabab, N., Mahmoudian, Z. G., & Alizadeh, Z. (2021). The potential renal toxicity of silver nanoparticles after repeated oral exposure and its underlying mechanisms. BMC Nephrology, 22(1), 228.
Pradell, T., Climent-Font, A., Molera, J., Zucchiatti, A., Ynsa, M. D., Roura, P., & Crespo, D. (2007). Metallic and nonmetallic shine in luster: An elastic ion backscattering study. Journal of Applied Physics, 101(10), 103518.
Saravanan, M., Barik, S. K., MubarakAli, D., Prakash, P., & Pugazhendhi, A. (2018). Synthesis of silver nanoparticles from Bacillus brevis (NCIM 2533) and their antibacterial activity against pathogenic bacteria. Microbial Pathogenesis, 116, 221–226.
Sarhan, O. M. M., & Hussein, R. M. (2014). Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat. International Journal of Nanomedicine, 9, 1505–1517.
Schelling, J. R. (2016). Tubular atrophy in the pathogenesis of chronic kidney disease progression. Pediatric Nephrology, 31(5), 693–706.
Sengupta, R., Singh, B., Chauhan, R. S., Srinivas, A., Patel, P., & Shinde, K. (2016). Practical consideration in toxicological testing. In R. Sengupta (Ed.), Regulatory toxicology: Essential practical aspects (pp. 6.1–6.71). Narosa Publishing House, New Delhi, India.
Singh, K. P., & Chauhan, R. S. (2008). CADRAD manual of staining techniques: Series 7. CADRAD, IVRI, Izatnagar, Bareilly, UP, India.
Srinivasan, C. (2007). Do Damascus swords reveal India's mastery of nanotechnology? Current Science, 92(3), 279–280.
Tiwari, R., Singh, R. D., Khan, H., Gangopadhyay, S., Mittal, S., Singh, V., & Srivastava, V. (2017). Oral subchronic exposure to silver nanoparticles causes renal damage through apoptotic impairment and necrotic cell death. Nanotoxicology, 11(5), 671–686.
Vasanth, S. B., & Kurian, G. A. (2017). Toxicity evaluation of silver nanoparticles synthesized by chemical and green route in different experimental models. Artificial Cells, Nanomedicine, and Biotechnology, 45(8), 1721–1727.
Zamil, R. Y. (2018). The effects of different concentrations of sliver nanoparticles on the kidneys of male albino mice. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS), 50(1), 190–203.
Zivic, F., Grujovic, N., Mitrovic, S., Ahad, I. U., & Brabazon, D. (2017). Characteristics and applications of silver nanoparticles. In Commercialization of nanotechnologies: A case study approach (pp. 227–273).
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