Profile of LH Release in Response to Intravenous Administration of Kisspeptin-10 in Postpartum Anoestrus Buffaloes
DOI:
https://doi.org/10.48165/ijvsbt.22.4.10Keywords:
Anoestrus, Buffalo, Kisspeptin, LHAbstract
The main objective of this study was to evaluate the effect of intravenous (IV) administration of different doses of kisspeptin (Kp) on the pattern of luteinizing hormone (LH) release in postpartum anoestrus buffaloes. Postpartum buffaloes weighing 510-520 kg (n=24; aged 4-6 years) were enrolled in this study. Non-cyclicity (anovulatory status) was confirmed by the absence of corpus luteum (CL) as determined by ultrasound scanning of the ovaries and low circulating P4 concentrations (<1.0 ng/ml) evaluated at 10-day intervals (on day -10 and day 0). Buffaloes were randomly assigned to one of four treatments: Kp @ 10 μg/kg body weight (Kp10, T1), 15 μg/kg (Kp15, T2), 20 μg/kg (Kp20, T3), or Normal saline solution (equivalent volume of kisspeptin solution, control), all administered by IV injection. A dose-dependent response was observed during induced LH release (p<0.05) in the treated animals. Nevertheless, the highest LH concentration was obtained in the Kp15 dose (2.4±0.03 ng/mL). In all Kp treatment groups, LH concentration rose 20 min post-kisspeptin administration, peaked at 40 min, and declined thereafter. The early onset of the LH surge (in a dose-dependent manner) after Kp treatment, together with its short duration, suggests that the effects of Kp were likely due to pituitary action. Finally, kisspeptin at 15 μg/kg body weight was the optimized dose for postpartum anoestrus buffaloes as it elicits the highest LH peak, and this dose can be used to formulate new therapeutic strategies for treating sub-fertile buffaloes.
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