Histomorphometric Analysis and Role of Immunoglobulins in Small Intestine of Mice Infected with Heterologous Rotavirus A
DOI:
https://doi.org/10.48165/ijvsbt.22.5.16Keywords:
Immunoglobulin’s, Mice, Morphometry, RotavirusAbstract
Homologous rotavirus (RV) causes acute severe enteritis in mammals, whereas only restricted replication occurs for heterologous RV in the intestine leading to mild enteritis in animals. To understand the role of immunoglubulins and related intestinal morphometry, day-old suckling Swiss albino mice pups (n=45) were randomly divided into two groups. The treatment group of pups (n=30) were orally inoculated with 100 µL of heterologous rotavirus A (RVA) containing 5x 106.5 TCID50 particles/mL per pup. The control group (n=15) received same volume of sterile phosphate buffered saline. The pups (6 each from treated and 3 from control group) were sacrificed at 1, 2, 4, 7, and 14 days-post infection (DPI). RT-PCR amplification of VP6 gene in rectal contents revealed 227 bp amplicon products of RVA from 1st to 7th DPI in the infected group. The level of sIgA and IgG immunoglobulins in rectal contents showed significantly decreasing trends from2nd DPI to 7th DPI in the infected group of mice. The morphometry of jejunum and ileum demonstrated shortening of the villi, relative decrease in the villous height, villous width, and villous: crypt ratio, while gradual increase in the crypt depth was noted from 1st to 14th DPI in the intestine segments. The histomorphometry of small intestine of suckling mouse model and level of immunoglobulins suggest that the heterologous RVA strains are less pathogenic to induce pathology in intestine.
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Blutt, S. E., Warfield, K. L., Lewis, D. E., & Conner, M. E. (2002). Early response to rotavirus infection involves massive B cell activation. The Journal of Immunology, 168(11), 5716–5721.
Boshuizen, J. A., Reimerink, J. H., Korteland-van Male, A. M., van Ham, V. J., Koopmans, M. P., Büller, H. A., Dekker, J., & Einerhand, A. W. (2003). Changes in small intestinal homeostasis, morphology, and gene expression during rotavirus infection of infant mice. Journal of Virology, 77(24), 13005–13016.
Cicchese, J. M., Evans, S., Hult, C., Joslyn, L. R., Wessler, T., Millar, J. A., Marino, S., Cilfone, N. A., Mattila, J. T., Linderman, J. J., & Kirschner, D. E. (2018). Dynamic balance of pro- and anti-inflammatory signals controls disease and limits pathology. Immunological Reviews, 285(1), 147–167.
Du, J., Lan, Z., Liu, Y., Liu, Y., Li, Y., Li, X., & Guo, T. (2017). Detailed analysis of BALB/c mice challenged with wild type rotavirus EDIM provide an alternative for infection model of rotavirus. Virus Research, 228, 134–140.
Feng, N., Burns, J. W., Bracy, L., & Greenberg, H. B. (1994). Comparison of mucosal and systemic humoral immune responses and subsequent protection in mice orally inoculated with a homologous or a heterologous rotavirus. Journal of Virology, 68(12), 7766–7773.
Feng, N., Kim, B., Fenaux, M., Nguyen, H., Vo, P., Omary, M. B., & Greenberg, H. B. (2008). Role of interferon in homologous and heterologous rotavirus infection in the intestines and extraintestinal organs of suckling mice. Journal of Virology, 82(15), 7578–7590.
Feng, N., Yasukawa, L. L., Sen, A., & Greenberg, H. B. (2013). Permissive replication of homologous murine rotavirus in the mouse intestine is primarily regulated by VP4 and NSP1. Journal of Virology, 87(15), 8307–8316.
Fromantin, C., Piroth, L., Petitpas, I., Pothier, P., & Kohli, E. (1998). Oral delivery of homologous and heterologous strains of rotavirus to BALB/c mice induces the same profile of cytokine production by spleen cells. Virology, 244(2), 252–260.
Jiang, B., Gentsch, J. R., & Glass, R. I. (2002). The role of serum antibodies in the protection against rotavirus disease: An overview. Clinical Infectious Diseases, 34(10), 1351–1361.
Kamdi, B., Singh, R., Singh, V., Singh, S., Kumar, P., Singh, K. P., George, N., & Dhama, K. (2020). Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India. Microbial Pathogenesis, 145, 104165.
Kawagishi, T., Nurdin, J. A., Onishi, M., Nouda, R., Kanai, Y., Tajima, T., Ushijima, H., & Kobayashi, T. (2020). Reverse genetics system for a human group A rotavirus. Journal of Virology, 94(2), 10-1128.
Kim, H. J., Park, J. G., Matthijnssens, J., Lee, J. H., Bae, Y. C., Alfajaro, M. M., Park, S. I., Kang, M. I., & Cho, K. O. (2011). Intestinal and extra-intestinal pathogenicity of a bovine reassortant rotavirus in calves and piglets. Veterinary Microbiology, 152(3–4), 291–303.
Kushnir, N., Bos, N. A., Zuercher, A. W., Coffin, S. E., Moser, C. A., Offit, P. A., & Cebra, J. J. (2001). B2 but not B1 cells can contribute to CD4+ T-cell-mediated clearance of rotavirus in SCID mice. Journal of Virology, 75(12), 5482–5490.
Lin, J. D., Feng, N., Sen, A., Balan, M., Tseng, H. C., McElrath, C., Smirnov, S. V., Peng, J., Yasukawa, L. L., Durbin, R. K., & Durbin, J. E. (2016). Distinct roles of type I and type III interferons in intestinal immunity to homologous and heterologous rotavirus infections. PLoS Pathogens, 12(4), e1005600.
Luna, L. G. (1972). Histological staining methods of the Armed Forces Institute of Pathology (3rd ed.). McGraw-Hill Book Co.
Mondal, A., Sharma, K., Malik, Y. S., & Joardar, S. N. (2013). Detection of group A rotavirus in faeces of diarrhoeic bovine, porcine, and human population from eastern India by reverse transcriptase–polymerase chain reaction. Population, 2013, 9–16.
Premkumar, P., Lopman, B., Ramani, S., Paul, A., Gladstone, B., Muliyil, J., Mukhopadhya, I., Parashar, U., & Kang, G. (2014). Association of serum antibodies with protection against rotavirus infection and disease in South Indian children. Vaccine, 32, 55–A61.
Ramig, R. F. (2004). Pathogenesis of intestinal and systemic rotavirus infection. Journal of Virology, 78(19), 10213–10220.
Reimerink, J. H., Boshuizen, J. A., Einerhand, A. W., Duizer, E., van Amerongen, G., Schmidt, N., & Koopmans, M. P. (2007). Systemic immune response after rotavirus inoculation of neonatal mice depends on source and level of purification of the virus: Implications for the use of heterologous vaccine candidates. Journal of General Virology, 88(2), 604–612.
Westerman, L. E., McClure, H. M., Jiang, B., Almond, J. W., & Glass, R. I. (2005). Serum IgG mediates mucosal immunity against rotavirus infection. Proceedings of the National Academy of Sciences, 102(20), 7268–7273.
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