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Home▶ Journals▶ Indian Journal of Veterinary Sciences and Biotechnology▶Archive▶Vol. 22 No. 3 (2026)▶Molecular Detection and Phylogenetic Analysis of…
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Molecular Detection and Phylogenetic Analysis of Mycoplasma conjunctivae from Ocular Infections of Goats in and around Tirupati Region

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Sreelekha Madiri — M.V.Sc Scholar, Department of Veterinary Microbiology, College of Veterinary Science, SVVU, Tirupati-517502, Andhra Pradesh, India.
Vijayalakshmi Siddavatam — Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Proddatur-516361 Andhra Pradesh, India
Nagendra Reddy Topireddy — Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502, Andhra Pradesh, India
Sujatha Karmala — Department of Veterinary Pathology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502, Andhra Pradesh, India
Suresh Yasarla — Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502, Andhra Pradesh, India
Published 22 Apr 2026 Vol. 22 No. 3 (2026), pp. 171-174 ● Open access
❝ How to cite 🔗 https://doi.org/10.48165/ijvsbt.22.3.34
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Abstract

Mycoplasma-associated infectious keratoconjunctivitis (IKC) represents one of the common ocular infections among ruminants.  Mycoplasma conjunctivae mainly causes infectious kerato-conjunctivitis (IKC) in goats, representing a highly transmissible condition  marked by differing levels of visual impairment in affected individuals. In the current study, a total of 39 conjunctival swabs were  collected from affected goats in and around the Tirupati region. Molecular detection was done by genus-specific and species-specific  PCR targeting the 16S rRNA gene, yielding 270 bp and 748 bp products, respectively. Phylogenetic analysis of the partial 16S rRNA gene  sequence of one of the obtained Mycoplasma conjunctivae isolates from goats revealed the genetic relatedness to distinct clade formed  by sheep ocular swab isolate from Brazil (MK 656520.1) and clustered with a goat isolate from Kerala, India (MW425380.1).

KeywordsGoats, Infectious keratoconjunctivitis, Molecular Detection, Mycoplasma conjunctivae, Phylogenetic analysis.

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