Ultrastructural Morphology and Molecular Identification of Laemobothrion maximum Lice (Phthiraptera: Laemobothriidae) from Brahminy Kite (Haliastur indus) in India
DOI:
https://doi.org/10.48165/ijvsbt.22.1.26Keywords:
Brahminy kite, Cytochrome c oxidase subunit-I (COI) gene, Electron microscopy, Laemobothrion, Morphometry, SensillaAbstract
Laemobothrion maximum is one of the largest known avian lice, predominantly parasitizing raptors but detailed morphological and molecular studies from India are scarce. A juvenile Brahminy kite rescued was presented with active lice infestation to VCRI, Orathanadu (TN, India). Lice were collected, morphologically examined and processed for scanning electron microscopy (SEM) and molecular confirmation. Morphological examination identified the specimens as L. maximum, measuring 10-11 mm, with a flattened head, sculptured temples, U-shaped sitophore sclerite, cephalic ctenidia and specialized tarsal claws. SEM revealed ultrastructural adaptations including mandibular cuticular projections, diverse trichoid and basiconic sensilla, reticulated abdominal microtrichial patterns. Molecular identification using mitochondrial cytochrome c oxidase subunit-I (COI) gene amplification confirmed species identity with high accuracy. This is the first integrated morphological, ultrastructural and molecular description of L. maximum infesting Brahminy kite in India. The findings provide insights into its identification features, host specificity and adaptive traits, offering a valuable baseline for future taxonomic, ecological and phylogenetic studies on avian lice.
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References
Arya, A. K., Bachheti, A., Sethi, V. K., & Joshi, K. K. (2024). Birds and environment: A multidisciplinary approach to ecological, behavioural and conservation issues. BMC Zoology, 9, 7.
Athira, A. K., Karthik, M., Meghna, P., Silambarasan, R., Navaz, S., & Latha, B. R. (2019). A report on the occurrence of Laemobothrion maximum in black kites (Milvus migrans). Journal of Veterinary Parasitology, 33(2), 50–52.
Clayton, D. H. (1990). Mate choice in experimentally parasitized rock doves: Lousy males lose. American Zoologist, 30(2), 251–262.
Clayton, D. H., Bush, S. E., & Johnson, K. P. (2009). Coevolution of life on hosts: Integrating ecology and history. University of Chicago Press, Chicago, USA.
Dalgleish, R. C., Price, R. D., Hellenthal, R. A., & Palma, R. L. (2006). The chewing lice: World checklist and biological overview. Illinois Natural History Survey Special Publication No. 24, 1–501.
Dik, B., & Ozkayhan, M. A. (2007). Mallophaga species on long-legged buzzards (Buteo rufinus) in Turkey. Türkiye Parazitoloji Dergisi, 31, 298–301.
Hatem, A. N., Abou Turab, M. K., Abdul-Zahra, H. K., & Muhammad, M. J. (2021). A survey of chewing lice of some raptors in southern Iraq, with remarks on prevalence and occurrence. Iraqi Journal of Veterinary Sciences, 35(2), 239–244.
Inci, A., Dik, B., Kibar, M., Yildirim, A., & Duzlu, O. (2010). Chewing lice (Phthiraptera) species on wild birds in the Cappadocia region, Turkey. Türkiye Parazitoloji Dergisi, 34, 174–178.
Jeyathilakan, N., Latha, B. R., Bino Sundar, S. T., & Abdul Basith, S. (2012). Occurrence of Laemobothrion maximum in greater coucal. Journal of Parasitic Diseases, 36(1), 120–121.
Jeyathilakan, N., Muthusami, P., & Senthil, K. (2021). Lice infestation in a Brahminy kite (Haliastur indus: Accipitriformes). The Indian Journal of Veterinary and Animal Sciences, 50, 71–73.
Krone, O., & Cooper, J. E. (2002). Parasitic disease. In J. E. Cooper (Ed.), Birds of prey: Health and diseases (pp. 105–120). Iowa State Press, Ames, USA.
Kushwaha, S. (2015). Mallophaga species on long-billed vultures (Gyps indicus) in Bundelkhand region of India and remarkable defense mechanisms of vultures against them. Journal of Wildlife Research, 3(4), 30–39.
Lade, D., Verma, R., Podhade, D. N., Das, G., Sinha, Y. K., & Jawre, S. (2023). Occurrence of Laemobothrion maximum and Degeeriella fulva lice infestation in black kites (Milvus migrans). Journal of Veterinary Parasitology, 37(1), 60–63.
Martín-Mateo, M. P. (2002). Fauna ibérica. Mallophaga, Amblycera (Vol. 20). Consejo Superior de Investigaciones Científicas, Madrid.
Mey, E. (2003). On the development of animal louse systematics (Insecta, Phthiraptera) up to the present day. Rudolstädter Naturhistorische Schriften, 11, 115–134.
Pérez, T. M., Granados, T. E., & Ruiz, I. (1995). The morphology of Laemobothrion (Laemobothrion) maximum (Phthiraptera: Laemobothriidae). Parassitologia, 37, 45–51.
Price, R. D., Hellenthal, R. A., Palma, R. L., Johnson, K. P., & Clayton, D. H. (2003). The chewing lice: World checklist and biological overview. Illinois Natural History Survey, Champaign.
Scopoli, J. A. (1763). Entomologia Carniolica exhibens insecta Carnioliae indigena et distributa in ordines, genera, species, varietates, methodo Linneana. Vindobonae. 36 + 420 pp., 43 plates.
Soulsby, E. J. L. (1982). Helminths, arthropods and protozoa of domesticated animals (7th ed.). ELBS, London.
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