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Research Article

Improved In Vitro Maturation of Goat Oocytes using Cysteamine and Epidermal Growth Factor-Enriched Media

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Malasri Gond — Department of Animal Reproduction Gynaecology & Obstetrics, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India
Rajeshwar Gangaram Bijurkar — Department of Veterinary Clinical Complex, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India
Manik Kishanrao Tandle — Department of Animal Reproduction Gynaecology & Obstetrics, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India
Venkanagouda Doddagoudar — Department of Animal Reproduction Gynaecology & Obstetrics, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India
Shrikant Kulkarni — Department of Veterinary Physiology and Biochemistry, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India
Shesharao Rathod — Buffalo Research and Information Center, Dornahalli Shahpur, Yadgir, KVAFSU, Karnataka, India
Published 8 Nov 2025 Vol. 21 No. 6 (2025), pp. 99-103 ● Open access
❝ How to cite 🔗 https://doi.org/10.48165/ijvsbt.21.6.18
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Abstract

The current study was performed to see the effect of cysteamine and epidermal growth factor (EGF) on in vitro maturation of goat  oocytes (n=8516). The four maturation media used in the study were: Control (TCM-199 + FSH 1 µg/mL + 20 IU/mL hCG + 1 μg/mL  Estradiol + Gentamicin 50 μg/mL + 10% EGS); Group I (Control + 100 µM/mL Cysteamine); Group II (Control + 20 ng/mL EGF) and Group  III (Control + 100 µM/mL Cysteamine and 20 ng/mL EGF), involving 1591, 1252, 1242, and 1214 oocytes, respectively, for 50, 33, 31,  and 30 trials. Later the oocytes were incubated in CO2 incubator for 27 h at 38.5°C, 5% CO₂, and 90-95% relative humidity. The in vitro maturation of goat oocytes, assessed based on cumulus expansion (77.02±2.24 to 77.56±1.57 vs 52.63±1.84%) and first polar body  extrusion (55.01±3.00 to 64.11 ±1.93 vs 31.64±2.68%) was significantly higher (p≤0.05) in Group I, II and III compared to Control group.  In summary, the addition of cysteamine and epidermal growth factor alone or in combination in the maturation media had a beneficial  effect on maturation rate in goat oocytes. 

 

KeywordsCysteamineEpidermal growth factorIn vitro maturationGoat

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