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Home▶ Journals▶ Indian Journal of Veterinary Sciences and Biotechnology▶Archive▶Vol. 21 No. 5 (2025)▶Effect of Oxygen Tension on In Vitro Maturation of Buffalo…
Indian Journal of Veterinary Sciences and Biotechnology
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Research Article

Effect of Oxygen Tension on In Vitro Maturation of Buffalo Cumulus Oocyte Complexes

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Meet R Patel — Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Junagadh-362001, Gujarat, India
Suryakant S Parikh — Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Junagadh-362001, Gujarat, India
Vishal S Suthar — Gujrat Biotechnology University, Gandhinagar-382010, Gujarat, India
Kiran H Parmar — Pashupalan Sanshodhan Kendra, Kamdhenu University, Ramna Muvada, Gujarat, India
Dwani Jhala — Gujarat Biotechnology Research Centre, Gandhinagar-382010, Gujarat, India
Ruchir D Pranami — Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Junagadh-362001, Gujarat, India
Munja V Hun — Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Junagadh-362001, Gujarat, India
Published 6 Sep 2025 Vol. 21 No. 5 (2025), pp. 129-132 ● Open access
❝ How to cite 🔗 https://doi.org/10.48165/ijvsbt.21.5.24
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Abstract

This study investigated the effect of oxygen (O2) tension during in vitro maturation (IVM) of buffalo cumulus oocyte complexes (COCs).  Buffalo ovaries (n=337) were collected from a slaughterhouse of Ahmedabad Municipal Corporation, Ahmedabad. A total of 801 COCs  were retrieved using aspiration method using 18 G 1.5’ needle and 5 mL syringe. The COCs were graded into four groups based on  cumulus layers. Two IVM conditions were compared: a benchtop incubator with 5% O2 and a conventional incubator with ambient O2 (20%). The COCs of the first three grades were randomly allocated to two groups and the effect on IVM was evaluated using chi square  test. The 5% O2 group exhibited a higher maturation rate (89.00%, 365/410) compared to the 20% O2 group (73.66%, 288/391), though  the difference was not statistically significant (p=0.21). The findings suggest that lower O2 tension during IVM may enhance COCs  maturation, potentially improving embryo development outcomes. 

 

KeywordsBenchtop incubatorBuffalo oocytesConventional incubatorIVMO2 tension

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