Abstract
A pathological study on canine brain tissue suspected with rabies was carried out on necropsy cases received at the Department of Veterinary Pathology, Madras Veterinary College Chennai, during April to September, 2019. Twenty-seven dogs presented for necropsy were considered for the study. The clinical history, age, breed and sex wise distribution were recorded. Gross changes if any was observed and samples of brain tissues were collected for histopathological studies. Brain impression smears were stained with William’s modification of Van Gieson’s stain and observed for presence of Negri bodies. Out of 27 dogs examined eight cases showed presence of Negri bodies. No significant gross changes were observable except for congestion, oedema and hemorrhages. Histopathological studies revealed subdural hemorrhages, marked gliosis, perivascular lymphocytic cuffing, neutrophilic vacuolation and presence of intracytoplasmic eosinophilic inclusions in neurons of cerebral grey matter.
The full text of this article is available as a PDF.
Download PDF- Abd El Rahman, S., Elbeskawy, M., & Hamed, M.F. (2015). Detection of rabies virus and its pathological changes in brain of buffaloes in Egypt. Adv. Anim. Vet. Sci., 3(11), 588–593.Andadari, A.Y.D., Pratama, D.A.O.A., Hardian, A.B., & Untari, H. (2024). Histopathological analysis of rabies disease from 2014 to 2022: A literature review. J. Appl. Vet. Sci. Tech., 5(1), 93–98.
- Ashwini, M.A., Pattanaik, A., & Mani, R.S. (2024). Recent updates on laboratory diagnosis of rabies. Indian J. Med. Res., 159(1), 48–61.
- Beck, S., Gunawardena, P., Horton, D.L., Hicks, D.J., Marston, D.A., Ortiz-Pelaez, A., & Núñez, A. (2017). Pathobiological investigation of naturally infected canine rabies cases from Sri Lanka. BMC Vet. Res., 13(1), 99.
- Beigh, A.B., Sandhu, B.S., Singh, C.K., Gupta, K., & Sood, N.K. (2017). Histopathological lesions associated with rabies in nervous tissue of animals. Indian J. Vet. Pathol., 41(3), 179–183.
- Galán, A., Gamito, A., Carletti, B.E., Guisado, A., de las Mulas, J.M., Pérez, J., & Martín, E.M. (2014). Uncommon acute neurologic presentation of canine distemper in 4 adult dogs. Can. Vet. J., 55(4), 373–376.
- Hampson, K., Coudeville, L., Lembo, T., Sambo, M., Kieffer, A., Attlan, M., & Global Alliance for Rabies Control Partners for Rabies Prevention. (2015). Estimating the global burden of endemic canine rabies. PLoS Negl. Trop. Dis., 9(4), e0003709.
- Madhusudana, S.N., Subha, S., Thankappan, U., & Ashwin, Y.B. (2012). Evaluation of a direct rapid immunohistochemical test (dRIT) for rapid diagnosis of rabies in animals and humans. Virol. Sin., 27(5), 299–302.
- Markbordee, B., Iamohbhars, N., Shiwa-sudo, N., Kimitsuki, K., Espino, M.J.M., Nacion, L.B., & Park, C.H. (2024). Histopathological and immunohistochemical examination of the brains of rabid dogs in the Philippines. J. Vet. Med. Sci., 86(12), 1243–1251.
- Pharande, R., Majee, S.B., Bannalikar, A.S., Gandge, R.S., Dighe, R.V., Moregoankar, S.S., & Mukherjee, S. (2017). Comparative assessment of Seller’s staining test (SST), direct fluorescent antibody test and real-time PCR for diagnosis of rabies in dogs. Int. J. Curr. Microbiol. Appl. Sci., 6(6), 1222–1228.
- Pieracci, E.G. (2019). Vital signs: trends in human rabies deaths and exposures—United States, 1938–2018. MMWR Morb. Mortal. Wkly. Rep., 68, 524–528.
- World Health Organization (WHO). (2020). Rabies vaccines and immunoglobulins: WHO position paper. World Health Organization, Geneva. Available from: https://www.who.int/immunization/policy/position_papers/pp_rabies_summary_2018.pdf
- Ramkumar, P.K., Karthika, K., Saravanan, M., Premalatha, N., Prabu, P.C., Priya, R.J., & Veeraselvam, M. (2025). Unusual canine rabies manifesting as Horner’s syndrome: A call for expanded diagnostic and reservoir surveillance. Indian J. Vet. Sci. Biotechnol., 17(2), (In press).
- Suvarna, K.S., Layton, C., & Bancroft, J.D. (2018). Bancroft’s Theory and Practice of Histological Techniques (8th ed.). Elsevier Health Sciences, Philadelphia, USA.
Looking up citing articles…
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright © 2025 Indian Journal of Veterinary Sciences and Biotechnology