Abstract
The present study was conducted to investigate the efficacy of different feed additives (Monensin T1, cellulase 10,00,000 IU/g T2, xylanase 1,50,000 IU/g T3, chitosan T4, sodium bicarbonate T5, magnesium oxide T6, combination of sodium bicarbonate + magnesium oxide T7 and combination of cellulase + xylanase T8 added at the level of 30 mg/kg, 0.5%, 0.5%, 1%, 1%, 1%, 0.5% (Each) and 0.5% (Each) on DM basis, respectively) on in vitro degradability of total mixed ration prepared by taking maize hay, bajra straw and concentrate in the ratio of 30:30:40 and used as substrates for experiment. Rumen liquor was collected from two adult Surti goats 2 h post-feeding. Statistical analysis revealed that IVDMD (%), IVOMD (%), IVNDFD (%), IVADFD (%), TDOMR (%), IVTGP (mL/200 mg), SCFAs (mMol/200 mg), ME (MJ/kg) and NE (MJ/kg) were significantly (p<0.01) increased in TMR supplemented with combination of cellulase and xylanase (67.32±1.01, 69.97±1.29, 56.23±0.36, 53.01±1.64, 64.13±0.42, 32.92 ±0.62, 0.75±0.007, 6.16±0.09 and 3.74±0.05 respectively). This was followed by TMR supplemented with cellulase, xylanase and chitosan alone, which also had significantly (p<0.01) higher values of all these components as compared to control (52.34±0.31, 54.92±0.50, 41.48±1.63, 38.64±0.53, 49.41±1.20, 22.08±0.56, 0.49±0.01, 4.58±0.08 and 2.70±0.05, respectively). Hence a combination of cellulase and xylanase is recommended as feed additive in maize hay, bajra straw and concentrate based TMR.
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Download PDF- Anassori, E., Dalir-Naghadeh, B., Pirmohammadi, R., Taghizadeh, A., Asri-Rezaei, S., Farahmand-Azar, S., & Tahmoozi, M. (2012). In vitro assessment of the digestibility of forage-based sheep diet, supplemented with raw garlic, garlic oil and monensin. Veterinary Research Forum, 3(1), 5.
- AOAC. (2005). Official methods of analysis (18th ed.). Horwitz William Publication.
- Bach, A., Guasch, I., Elcoso, G., Duclos, J., & Khelil-Arfa, H. (2018). Modulation of rumen pH by sodium bicarbonate and a blend of different sources of magnesium oxide in lactating dairy cows submitted to a concentrate challenge. Journal of Dairy Science, 101(11), 9777–9788.
- Besharati, M., Palangi, V., Moaddab, M., Nemati, Z., & Ayaşan, T. (2021). Comparative effects of addition of monensin, tannic acid and cinnamon essential oil on in vitro gas production parameters of sesame meal. Journal of the Hellenic Veterinary Medical Society, 72(2), 2977–2988.
- Butaye, P., Devriese, L. A., & Haesebrouck, F. (2003). Antimicrobial growth promoters used in animal feed: Effects of less well-known antibiotics on Gram-positive bacteria. Clinical Microbiology Reviews, 16(2), 175–188.
- De Paiva, P. G., de Jesus, E. F., Del Valle, T. A., de Almeida, G. F., Costa, A. G. B. V. B., Consentini, C. E. C., & Rennó, F. P. (2016). Effects of chitosan on ruminal fermentation, nutrient digestibility, and milk yield and composition of dairy cows. Animal Production Science, 57(2), 301–307.
- Dias, A. O. C., Goes, R. H. T. B., Gandra, J. R., Takiya, C. S., Branco, A. F., Jacaúna, A. G., & Vaz, M. S. M. (2017). Increasing doses of chitosan to grazing beef steers: Nutrient intake and digestibility, ruminal fermentation, and nitrogen utilization. Animal Feed Science and Technology, 225, 73–80.
- Geoering, H. K., & Van Soest, P. J. (1970). Forage fibre analysis. Apparatus, reagents, procedure and some applications. Agricultural Handbook, 379.
- Goiri, I., Oregui, L. M., & Garcia-Rodriguez, A. (2010). Use of chitosans to modulate ruminal fermentation of a 50:50 forage-to-concentrate diet in sheep. Journal of Animal Science, 88(2), 749–755.
- ICAR. (2013). Nutrient requirements of sheep, goat and rabbit (3rd ed.). Indian Council of Agricultural Research.
- Kadam, S. J., Garg, D. D., Savsani, H. H., Patel, S. D., Patbandha, T. K., Karangiya, V. K., & Sanchaniya, J. M. (2024). Effect of varying levels of fibrolytic enzymes supplementation on digestibility of nutrients and plane of nutrition in Gir calves. The Indian Journal of Veterinary Science and Biotechnology, 20(6), 7–10.
- Karmakar, N. D. (2021). Effect of exogenous fibrolytic enzymes and soapnut (Sapindus mukorossi) shell powder on growth performance of Gir calves (M.V.Sc. Thesis). Junagadh Agricultural University.
- Li, C., Zhao, X., Cao, Y., Lei, Y., Liu, C., Wang, H., & Yao, J. (2013). Effects of chitosan on in vitro ruminal fermentation in diets with different forage to concentrate ratios. Journal of Animal and Veterinary Advances, 12(7), 839–845.
- Lopez, D., Vázquez-Armijo, J. F., López-Villalobos, N., Lee-Rangel, H. A., Salem, A. Z. M., Borquez-Gastelum, J. L., & Rojo-Rubio, R. (2016). In vitro gas production of foliage from three browse tree species treated with different dose levels of exogenous fibrolytic enzymes. Journal of Animal Physiology and Animal Nutrition, 100(5), 920–928.
- Lunagariya, P. M., Shah, S. V., Devalia, B. R., Patel, A. C., & Pandya, P. R. (2018). An in vitro dose optimization of exogenous fibrolytic enzymes in total mixed ration for crossbred cows. International Journal of Current Microbiology and Applied Science, 7(10), 330–338.
- Menke, K. H., & Steingass, H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28, 7–55.
- Morsy, T. A., Kholif, A. E., Kholif, S. M., Kholif, A. M., Sun, X., & Salem, A. Z. (2016). Effects of two enzyme feed additives on digestion and milk production in lactating Egyptian buffaloes. Annals of Animal Science, 16(1), 209.
- Patel, S. D., Garg, D. D., Savsani, H. H., Chavda, J. A., Sharma, A. K., & Kalam, S. M. (2025). In vitro rumen fermentation of maize hay and bajra straw based total mixed ration containing different feed additives. The Indian Journal of Veterinary Science and Biotechnology, 21(1), 21–24.
- Rajamma, K., Kumar, D. S., Rao, E. R., & Nath, D. N. (2015). In vitro evaluation of total mixed rations containing different roughage-concentrate ratios supplemented with or without fibrolytic enzymes. Animal Science, 9, 63–69.
- Shekhar, C., Thakur, S. S., & Shelke, S. K. (2010). Effect of exogenous fibrolytic enzymes supplementation on milk production and nutrient utilization in Murrah buffaloes. Tropical Animal Health and Production, 42, 1465–1470.
- Singh, D. N., Bohra, J. S., Tyagi, V., Singh, T., Banjara, T. R., & Gupta, G. (2022). A review of India’s fodder production status and opportunities. Grass and Forage Science, 77(1), 1–10.
- Snedecor, G. W., & Cochran, W. G. (1994). Statistical methods (8th ed.). Oxford and IBH.
- Tilley, J. M. A., & Terry, D. R. (1963). A two-stage technique for the in vitro digestion of forage crops. Grass and Forage Science, 18(2), 104–111.
- Vallejo, L. H., Salem, A. Z. M., Kholif, A. E., Elghangour, M. M. Y., Fajardo, R. C., Rivero, N., & Mariezcurrena, M. D. (2016). Influence of cellulase or xylanase on the in vitro rumen gas production and fermentation of corn stover. Indian Journal of Animal Science, 86(1), 70–74.
- Wencelova, M., Varadyova, Z., Mihalikova, K., Kisidayova, S., & Jalc, D. (2014). Evaluating the effects of chitosan, plant oils, and different diets on rumen metabolism and protozoan population in sheep. Turkish Journal of Veterinary & Animal Sciences, 38(1), 26–33
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