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Home▶ Journals▶ Indian Journal of Veterinary Sciences and Biotechnology▶Archive▶Vol. 21 No. 5 (2025)▶Emergence of ESBL-Producing Klebsiella oxytoca in Bovine…
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Research Article

Emergence of ESBL-Producing Klebsiella oxytoca in Bovine  Mastitic Milk: A Study in Gujarat, India

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Bhavesh I Prajapati — Department of Veterinary Public Health & Epidemiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385505, Banaskantha, Gujarat, India
Bhavin P Katira — Department of Veterinary Public Health & Epidemiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385505, Banaskantha, Gujarat, India
Ratn Deep Singh — Department of Veterinary Public Health & Epidemiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385505, Banaskantha, Gujarat, India
Kiran M Solanki — Department of Veterinary Public Health & Epidemiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385505, Banaskantha, Gujarat, India
Akshay R Bariya — Department of Veterinary Public Health & Epidemiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385505, Banaskantha, Gujarat, India
Kelvina J Patel — Department of Veterinary Public Health & Epidemiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385505, Banaskantha, Gujarat, India
Published 1 Sep 2025 Vol. 21 No. 5 (2025), pp. 7-11 ● Open access
❝ How to cite 🔗 https://doi.org/10.48165/ijvsbt.21.5.02
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Abstract

The present study reports first detection of extended spectrum β-lactamase (ESBL) producing Klebsiella oxytoca, an opportunistic  pathogen, from bovine milk samples collected from milk-shed regions of Gujarat. The study included a total of 270 bovine milk samples  comprising of 175 fresh milk sample from apparently healthy dairy animals and 95 mastitic milk samples. A total of five K. Oxytoca  isolates were identified based on results of cultural, biochemical and molecular techniques, all from mastitic milk samples only, yielding  an overall prevalence of 5.26 % (5/95). None of the milk sample from healthy animals was found positive for the presence of Klebsiella  oxytoca. Phenotypic characterization revealed that 80 % (4/5) isolates were ESBL producing Klebsiella oxytoca based on both results  of Combination Disc Diffusion test (CDDT) and HiCrome ESBL agar culture. Further, ESBL producing genes, viz., blaTEM and blaSHV were  found in one and four isolates, respectively, whereas virulent gene wabG was found in all five isolates. None of the K. Oxytoca isolates  was found positive for the presence of ESBL producing gene blaCTX-M-1, and virulent gene kfuBC. 

 

KeywordsBovine milkESBL producing genesKlebsiella oxytocaVirulence genes

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