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Indian Journal of Veterinary Sciences and Biotechnology
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Prevalence, Virulence Traits and Antimicrobial Resistance Pattern of Staphylococcus aureus Isolated from Chicken

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Amruta Doiphode — Department of Veteri nary Public Health, Krantisinh Nana Patil College of Veterinary Sci ence, Shirwal, Dist. Satara-412801, Maharashtra Animal and Fishery Sciences University (MAFSU), Nagpur-440001, India
Vikas Waskar — Department of Veterinary Public Health, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Dist. Satara-412801, Maharashtra Animal and Fishery Sciences University (MAFSU), Nagpur-440001, India.
Rahul Kolhe — Department of Veterinary Public Health, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Dist. Satara-412801, Maharashtra Animal and Fishery Sciences University (MAFSU), Nagpur-440001, India.
Bapurao Kadam — Department of Livestock Products Technology, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Dist. Satara-412801, MAFSU, Nagpur-440001, India.
Prashant Mhase — Department of Veterinary Microbiology, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Dist. Satara-412801, MAFSU, Nagpur-440001, India.
Published 15 Sep 2022 Vol. 18 No. 4 (2022), pp. 130-132 ● Open access
❝ How to cite 🔗 https://doi.org/10.48165/ijvsbt.18.4.26
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Abstract

Staphylococcus aureus isolated from chicken samples of retail market of Shirwal city exhibited 36% (18/50) prevalence, confirmed  biochemically as well as by polymerase chain reaction by employing 16s-rDNA and species-specific sau genes. None of the isolates were  found to possess virulence genes, viz., sea, seb, sec and sed. Antimicrobial resistance pattern revealed that 100% isolates were resistant  to 16 among 24 antibiotics, while 5 antibiotics showed more than 70% resistance, except for tobramycin (44.44%) and gentamicin,  streptomycin (38.89% each). All isolates were multidrug resistant (MDR). Screening for the presence of antimicrobial resistance genes  revealed the presence of aacA-D, ermA, tetK and tetM genes. None of the isolates carried mecA, mrsA, mrsB, vanA, vanB and ermC genes,  although phenotypic resistance was noted.  

KeywordsAntimicrobial resistanceChickenPrevalenceStaphylococcus aureusPolymerase Chain Reaction (PCR)Virulence Genes

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