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Effects of Coconut Water-Based Extender on Motility, Viability, Morphology and Membrane Integrity of Refrigerated (4°C) Canine Spermatozoa

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Abhay Kumar Meena — Department of Veterinary Gynaecology and Obstetrics, Post Graduate Institute of Veterinary Education and Research, Jaipur, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334001 (Rajasthan), India
Chandra Shekher Saraswat — Department of Veterinary Gynaecology and Obstetrics, Post Graduate Institute of Veterinary Education and Research, Jaipur, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334001 (Rajasthan), India
Sumit Prakash Yadav — Department of Veterinary Gynaecology and Obstetrics, Post Graduate Institute of Veterinary Education and Research, Jaipur, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334001 (Rajasthan), India
Published 7 Sep 2024 Vol. 20 No. 5 (2024), pp. 160-163 ● Open access
❝ How to cite 🔗 https://doi.org/10.48165/ijvsbt.20.5.33
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Abstract

The objective of this study was to determine the effect of a coconut water-based extender on the quality parameters of dog semen (n=24  ejaculates) during refrigeration (4°C) preservation up to 72 h. Four ejaculates per dog were collected from 6 dogs by digital manipulation.  All fresh ejaculates were evaluated for macroscopic and microscopic semen evaluation tests. The semen samples were then extended  in a coconut water-based extender using a split sample technique. The diluted semen was kept in a refrigerator for preservation at 4°C  and evaluated at 0, 24, 48, and 72 h for sperm quality parameters. The sperm motility significantly reduced over a period of time from  0 to 72 h. The live sperm percentage did not differ significantly at 0, 24 and 48 h of preservation. However, there was a significant drop  in live sperm percentage at 72 h. The percent sperm abnormalities were significantly increased from 24, 48 to 72 h of preservation. In  coconut water-based extender there was a significant difference in intact plasma membrane percentage at different time slots (0, 24,  48 and 72 h). It was concluded that coconut water-based extender can preserve canine sperm for up to 48 h while maintaining sperm  motility and viability above 50%. Sperm abnormalities remained within 20% and plasma membrane integrity above 60% both were  maintained up to 72 h of semen preservation. 

KeywordsCanine semenCoconut water-based semen extenderCooled storage

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