Abstract
The objective of the present study was to evaluate the diagnostic potential of pregnancy detection methods at an early stage in cattle using transrectal ultrasonography, per-rectal palpation and commercially available PAG based ELISA kit. The study was conducted on 116 crossbred cyclic cows, aged 3-8 years. The cows at estrus were artificially inseminated using 2 doses of quality semen at 10-12 h interval. They were subjected to trans-rectal ultrasonography and PAG test in randomized animals at around 28-35 days’ post-insemination. The animals were further examined by second trans-rectal ultrasonography and per-rectal palpation on day 45-50 post-AI. Ultrasonography was found to be more effective in diagnosing early pregnancy followed by PAG test around day 28 and per-rectal examination by day 45-50 post-insemination. However, PAG test was more accurate in diagnosing open cows at the earliest.
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