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Home▶ Journals▶ Indian Journal of Veterinary Sciences and Biotechnology▶Archive▶Vol. 20 No. 4 (2024)▶Antimicrobial Resistance and Biofilm Formation in Coagulase…
Indian Journal of Veterinary Sciences and Biotechnology
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Research Article

Antimicrobial Resistance and Biofilm Formation in Coagulase Negative Staphylococci Isolated from Bovine Clinical Mastitis

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Prakruti J Poshiya — Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-360311, Gujarat, India
Bhavesh B Javia — Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-360311, Gujarat, India
Sanjay N Ghodasara — Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-360311, Gujarat, India
Amit R Bhadaniya — Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-360311, Gujarat, India
Shobha Khambam — Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-360311, Gujarat, India
Published 1 Jul 2024 Vol. 20 No. 4 (2024), pp. 139-142 ● Open access
❝ How to cite 🔗 https://doi.org/10.48165/ijvsbt.20.4.29

Abstract

Bovine mastitis is a common and economically significant disease that affects dairy cows and buffaloes. Staphylococci are considered  as a most common cause of bovine mastitis. The present research work was undertaken with the objective to isolate and identify  coagulase-negative staphylococci (CoNS) by cultural, biochemical, and molecular methods from bovine mastitic milk samples, and to  study their antimicrobial resistance patterns as well as the biofilm-producing ability. The investigation was carried out on 281 bovine  clinical mastitis milk samples collected in and around Junagadh. Based on cultural and molecular methods, 34.87% (98/281) samples  yielded Staphylococcus isolates. Through the conventional standard tube coagulation test, 16.72% (47/281) isolates were identified as  CoNS. Mannitol fermentative patterns revealed 36.17% (17/47) CoNS isolates as fermentative, while 63.82% (30/47) CoNS isolates as  non-fermentative. CoNS isolates showed high resistance against penicillin-G (64.95%), followed by erythromycin (57.34%), while the  least resistance was observed against oxytetracycline (17.02%). Out of 47 CoNS isolates, 76.59% (36/47) were biofilm producers, while  23.40% (11/47) were non-biofilm producers.

KeywordsBiofilm,BovineClinical mastitis,CoNS.
  1. Bal, E.B.B., Bayar, S., & Bal, M.A. (2010). Antimicrobial susceptibilities of coagulase-negative staphylococci (CoNS) and streptococci from bovine subclinical mastitis cases. The Journal of Microbiology, 48(3), 267-274.
  2. Das, D., Panda, S.K., Jena, B., & Sahoo, A.K. (2018). Economic impact of subclinical and clinical mastitis in Odisha, India. International Journal of Current Microbiology and Applied Science, 7(3), 3651- 3654.
  3. De Buck, J., Ha, V., Naushad, S., Nobrega, D.B., Luby, C., Middleton, J.R., De Vliegher, S., & Barkema, H.W. (2021). Non-aureus staphylococci and bovine udder health: Current understanding and knowledge gaps. Frontiers in Veterinary Science, 8, 658031.
  4. Ebrahimi, A., Moatamedi, A., Lotfalian, S., & Mirshokraei, P. (2013). Biofilm formation, haemolysin production and antimicrobial susceptibilities of Streptococcus agalactiae isolated from the mastitis milk of dairy cows in Shahrekord district, Iran. Veterinary Research Forum, 4(4), 269-272.
  5. Gomes, F., & Henriques, M. (2016). Control of bovine mastitis: Old and recent therapeutic approaches. Current Microbiology, 72(4), 377-382.
  6. Kateete, D.P., Kimani, C.N., Katabazi, F.A., Okeng, A., Okee, M.S., Nanteza, A., Joloba, M.L., & Najjuka, F.C. (2010). Identification of Staphylococcus aureus: DNase and mannitol salt agar improve the efficiency of the tube coagulase test. Annals of Clinical Microbiology and Antimicrobials, 9, 23.
  7. Kenar, B., Kuyucuoglu, Y., & Seker, E. (2012). Antibiotic susceptibility of coagulase-negative staphylococci isolated from bovine subclinical mastitis in Turkey. Pakistan Veterinary Journal, 32(3), 390-393.
  8. Klibi, A., Maaroufi, A., Torres, C., & Jouini, A. (2018). Detection and characterization of methicillin-resistant and susceptible coagulase-negative staphylococci in milk from cows with clinical mastitis in Tunisia. International Journal of Antimicrobial Agents, 52(6), 930-935.
  9. Kudinha, T., & Simango, C. (2002). Prevalence of coagulase-negative staphylococci in bovine mastitis in Zimbabwe. Journal of the South African Veterinary Association, 73(2), 62-65.
  10. Lee, Y.J., & Lee, Y.J. (2022). Characterization of biofilm producing coagulase-negative staphylococci isolated from bulk tank milk. Veterinary Sciences, 9(8), 430-439.
  11. Mahmoud, A.K.A., Khadr, A.M., Elshemy, T.M., Hamoda, H.A., & Mohamed, E.S. (2015). Role of Coagulase positive and Coagulase negative staphylococci in bovine mastitis with special reference to some of their virulence genes and antimicrobial sensitivity. Alexandria Journal for Veterinary Sciences, 46(1), 83-89.
  12. Mason, W.J., Blevins, J.S., Beenken, K., Wibowo, N., Ojha, N., & Smeltzer, M.S. (2001). Multiplex PCR protocol for the diagnosis of staphylococcal infection. Journal of Clinical Microbiology, 39(9), 3332-3338.
  13. Melchior, M.B., Fink-Gremmels, J., & Gaastra, W. (2007). Extended antimicrobial susceptibility assay for Staphylococcus aureus isolates from bovine mastitis growing in biofilms. Veterinary Microbiology, 125(1-2), 141-149.
  14. Moniri, R., Dastehgoli, K., & Akramian, A. (2007). Increasing resistant coagulase negative staphylococci in bovine clinical mastitis. Pakistan Journal of Biological Sciences, 10(15), 2465- 2469.
  15. Parasana, D.K., Javia, B.B., Barad, D.B., Fefar, D.T., & Ghodasara, S.N. (2023). Detection of Biofilm Forming Streptococcus species from Bovine Mastitis. Indian Journal of Veterinary Sciences & Biotechnology, 19(1), 106-108.
  16. Patel, I., Patel, V., Thakkar, A., & Kothari, V. (2014). Microbial biofilms: Microbes in social mode. International Journal of Agricultural and Food Research, 3(2), 34-49.
  17. Pitkala, A., Haveri, M., Pyoral, S., Myllys, V., & Honkanen-Buzalski, T. (2004). Bovine mastitis in Finland 2001: Prevalence, distribution of bacteria, and antimicrobial resistance. Journal of Dairy Science, 87(8), 2433-2441.
  18. Preethirani, P.L., Isloor, S., Sundareshan, S., Nuthanalakshmi, V., Deepthikiran, K., Sinha, A. Y., Rathnamma, D., Nithin Prabhu, K., Sharada, R., Mukkur, T.K., & Hegde, N.R. (2015). Isolation, biochemical and molecular identification, and in-vitro antimicrobial resistance patterns of bacteria isolated from bubaline subclinical mastitis in South India. Plos One, 10(11), e0142717.
  19. Radostits, O.M., Gay, C.C., Blood, D.C., & Hinchcliff, K.W. (2000). Mastitis. In: Veterinary Medicine, A textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses, 9th ed., W.B. Saunders, London.
  20. Singh, R.D., Prajapati, B.I., Mody, S.K., Patel, H.B., Deokar, S.S., Solanki, B.A., Chauhan, P.M., & Momin, R.R. (2017). Antibiogram of CoPS and CoNS isolated from bovine mastitis in Banaskantha district of Gujarat, India. International Journal of Current Microbiology and Applied Sciences, 6(10), 564-569.
  21. Taponen, S., Simojoki, H., Haveri, M., Larsen, H. D., & Pyorala, S. (2006). Clinical characteristics and persistence of bovine mastitis caused by different species of coagulase-negative staphylococci identified with API or AFLP. Veterinary Microbiology, 115(1-3), 199-207.
  22. Tenhagen, B.A., Koster, G., Wallmann, J., & Heuwieser, W. (2006). Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. Journal of Dairy Science, 89(7), 2542-2551.
  23. Thakur, P., Nayyar, C., Tak, V., & Saigal, K. (2017). Mannitol-fermenting and tube coagulase-negative staphylococcal isolates: Unraveling the diagnostic dilemma. Journal of Laboratory Physicians, 9(1), 65-66.
  24. Tremblay, Y.D., Lamarche, D., Chever, P., Haine, D., Messier, S., & Jacques, M. (2013). Characterization of the ability of coagulase-negative staphylococci isolated from the milk of Canadian farms to form biofilms. Journal of Dairy Science, 96(1), 234-246.
  25. Viguier, C., Arora, S., Gilmartin, N., Welbeck, K., & O’Kennedy, R. (2009). Mastitis detection: Current trends and future perspectives. Trends in Biotechnology, 27(8), 486-493.

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