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Indian Journal of Veterinary Sciences and Biotechnology
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Research Article

Antibiogram Pattern of Bacterial Isolates from Bovine and Bubaline Semen in Karnataka

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Nithyananda V C — Department of Animal Husbandry & Veterinary Services, Govt. of Karnataka, India
Shivaraj Murag — Southern Regional Disease Diagnostic Laboratory (SRDDL), IAH&VB, Hebbal-560024, Bengaluru, KVAFSU, Bidar, India
Chandrashekar K M — Department of Veterinary Microbiology, Veterinary College, Gadag-582101, KVAFSU, Bidar, India
Shivashankar B P
Isloor S — Department of Veterinary Microbiology, Veterinary College, Hebbal-560024, Bengaluru, KVAFSU, Bidar, India
Rathnamma D — Department of Veterinary Microbiology, Veterinary College, Hebbal-560024, Bengaluru, KVAFSU, Bidar, India
Published 1 Jul 2024 Vol. 20 No. 4 (2024), pp. 25-28 ● Open access
❝ How to cite 🔗 https://doi.org/10.48165/ijvsbt.20.4.06
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Abstract

To avert bacterial contamination in semen, antibiotics are added. Inspite of addition of conventional antibiotics, extended and frozen semen  samples from bulls have been reported to contain a number of bacterial contaminants which may be due to the antimicrobial resistance  because of indiscriminate usage of antibiotics. The present study was conducted to know the antibiotic susceptibility pattern of the bacterial  isolates from neat semen and frozen semen straws of cattle and buffalo bulls, collected from three semen stations (Farm A, B & C) of Karnataka.  A total of twenty eight Holstein Friesian (HF) bulls, fourteen Murrah and two Surti buffalo bulls were selected for the study. Three time sample  collections from each farm at a gap of 15 days were carried out. A total of 205 bacterial isolates were obtained from 264 samples of neat and  frozen semen from three different farms (Farm A 49, Farm B 63 and Farm C 93). All isolates were, highly sensitive to newer generation antibiotics  Piperacillin-Tazobactam, Imipenem and completely resistant to routinely used antibiotics Penicillin G, and Streptomycin. 

KeywordsAntibiotics,Frozen semenNeat semenPenicillinStreptomycin.

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