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ANALYSIS OF POLYPEPTIDE PROFILES OF STRONGYLID NEMATODES USING SODIUM DODECYL SULPHATE POLYACRYLAMIDE GEL ELECTROPHORESIS

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S Arunkumar — Department of Veterinary Parasitology, Madras Veterinary College Tamil Nadu Veterinary and Animal Sciences University, Chennai - 600 007
R L Sharma — Department of Veterinary Parasitology, Madras Veterinary College Tamil Nadu Veterinary and Animal Sciences University, Chennai - 600 007
Published 10 Oct 2010 Vol. 5 No. 4 (2010), pp. 41-43 ● Open access
❝ How to cite 🔗 https://doi.org/10.48165/
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Abstract

Polypeptide profile of soluble extract antigens (SEA) of referral nematodes viz., Haemonchus contortus, Bunostomum trigonocephalum and Oesophagostomum columbianum were analysed by sodium dodecyl sulphate polyacarylamide gel electrophoresis (SDS-PAGE). The SEA of H. contortus showed bands at 24.0, 29.0, 35.0, 43.0, 60.0, 94.0 and 106 kDa molecular weight whereas the SEA of B. trigonocephalum showed bands at 21.0, 29.0, 30.0, 47.0, 60.0, 94.0 and 101 kDa size. The SEA of O. columbianum revealed polypeptide bands at 29.0, 39.0, 54.0, 60.0, 94.0 and 112 kDa. It was also revealed that a common polypeptide bands at 29.0, 60.0 and 94.0 kDa were present in all three antigens. 

KeywordsAntigenic profilesgastro intestinal nematodesSDS-PAGE

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