EVALUATION OF SPP. AND ESSENTIAL OILS TO MINIMIZE GROWTH OF CAUSING SOFT ROT OF STRAWBERRY
DOI:
https://doi.org/10.48165/jpds.2026.21.01.03Keywords:
Antagonists, Essential oils, Rhizopus stolonifer, StrawberryAbstract
Strawberry is a highly perishable fruit and is highly susceptible to pathogenic fungi, resulting in significant nutritional and economic losses. In this study, healthy and infected strawberry fruits were collected during March and April of 2024 and 2025 from different markets of Alipurduar, West Bengal, India. Soft rot was identified as a common postharvest disease caused by Rhizopus stolonifer, which was confirmed through microscopic examination of the isolated fungus and rDNA ITS sequencing. Pathogenicity was further verified by inoculating healthy fruits and re-isolating the pathogen. As an alternative to chemical fungicides, sustainable management strategies were evaluated using four Trichoderma species (T. harzianum, T. koningiopsis, T. virens, and one unidentified Trichoderma species) along with six essential oils against R. stolonifer. All Trichoderma isolates inhibited pathogen growth, with T. harzianum and T. virens showing the greatest effectiveness when inoculated one or two days before the pathogen. Among the six essential oils tested, cinnamon, citronella, and clove oils completely inhibited pathogen growth in vitro even after 17 days of incubation. Application of these essential oils on strawberry fruits also reduced disease development, with cinnamon oil showing the highest level of inhibition, followed by citronella and clove oils. These findings indicate that selected Trichoderma species and essential oils have considerable potential as eco-friendly alternatives for the sustainable management of postharvest soft rot of strawberry caused by Rhizopus stolonifer.
References
Abd-Elkader, D. Y., Salem, M. Z. M., Komeil, D. A., Al-Huqail, A. A., Ali, H. M., Salah, A. H., Akrami, M., & Hassan, H. S. (2021). Post-harvest enhancing and control of Botrytis cinerea on strawberry fruits using low-cost and eco-friendly natural oils. Agronomy, 11(6), 1246. https://doi.org/10.3390/agronomy11061246
Adl, E., Jahani, M., & Aminifard, M. H. (2024). Investigating the antifungal effects of essential oils on Aspergillus spp. in strawberry (Fragaria ananassa Duchesne) fruit. Journal of Horticultural and Postharvest Research, 7(2), 141–154.
Allagui, M. B., Moumni, M., & Romanazzi, G. (2023). Antifungal activity of thirty essential oils to control pathogenic fungi of postharvest decay. Antibiotics, 13(1), 28.
Baviskar, R. (2024). Prevalence and diversity of spoilage fungi in post-harvest strawberries from Vashi Fruit Market, Navi Mumbai. World Journal of Pharmaceutical Research, 14(3), 1192–1197.
Bhale, U. N. (2020). Antagonistic conflict of Trichoderma harzianum against fruit rot pathogens. African Journal of Biological Sciences, 2(4), 92–98.
Boubaker, H., Karim, H., El Hamdaoui, A., Msanda, F., Leach, D., Bombarda, I., & Aoumar, A. A. B. (2016). Chemical characterization and antifungal activities of four Thymus essential oils against postharvest fungal pathogens of citrus. Industrial Crops and Products, 86, 95–101.
Das, J. (2016). Efficacy of Trichoderma against violet root rot disease of tea caused by Sphaerostilbe repens. International Journal of Life Sciences, 4(4), 579–582.
Das, S. S., & Das, J. (2024). Efficacy of antagonistic bacteria against postharvest fungal rot pathogens of Citrus reticulata Blanco. Journal of Mycopathological Research, 62(4), 831–835.
De Corato, U. (2019). Postharvest control of strawberry fruit soft rot by Laminaria digitata extracts. Open Access Journal of Agricultural Research, 4(1), 1–13.
Demir, S., Durak, E. D., Gune, H., Boyno, G., Mulet, J. M., Danesh, Y. R., & Porcel, R. (2023). Biological control of three fungal diseases in strawberry (Fragaria × ananassa) with arbuscular mycorrhizal fungi. Agronomy, 13(9), 2439.
Dwivedi, M., Rai, R. K., & Singh, P. (2024). Fungal diversity associated with strawberry fruit loss in the Gorakhpur Division, U.P., India. Agricultural Science Digest, 50(2), 79–87.
Dwivedi, M., Rai, R. K., & Singh, P. (2025). Evaluating innovative strategies for managing strawberry fruit rot diseases: Insight from current research. Studies in Fungi, 10(1).
Huang, R., Li, G. Q., Zhang, J., Yang, L., Che, H. J., Jiang, D. H., & Huang, H. C. (2011). Control of postharvest fruit rot of strawberry by volatile organic compounds of Candida intermedia. Phytopathology, 101(7), 859–869.
Kahramanoğlu, Y., Panfilova, O., Kesimci, T. G., Bozhuyuk, A. U., Gurbuz, R., & Alptekin, H. (2022). Control of postharvest gray mold of strawberry caused by Botrytis cinerea and improvement of fruit storability through Ziziphora clinopodioides L. and Origanum onites L. volatile essential oils. Agronomy, 12, 389. https://doi.org/10.3390/agronomy12020389
Lopez-Reyes, J. G., Spadaro, D., Gullino, M. L., & Garibaldi, A. (2010). Efficacy of plant essential oils on postharvest control of rot caused by fungi on four apple cultivars in vivo. Flavour and Fragrance Journal, 25(3), 171–177.
Modrzewska, M., Błaszczyk, L., Stępień, Ł., Urbaniak, M., Waśkiewicz, A., Yoshinari, T., & Bryła, M. (2022). Trichoderma versus Fusarium: Inhibition of pathogen growth and mycotoxin biosynthesis. Molecules, 27(23), 8146. https://doi.org/10.3390/molecules27238146
Oliveira Filho, J. G., Silva, G., Egea, M. B., Azeredo, H. M. C., & Ferreira, M. D. (2021). Essential oils as natural fungicides to control Rhizopus stolonifer-induced spoilage of strawberries. Biointerface Research in Applied Chemistry, 11, 13244–13251.
Quarshi, H. Q., Ahmed, W., Azmant, R., Chendouh-Brahmi, N., Quyyum, A., & Abbas, A. (2023). Post-harvest problems of strawberry and their solutions. In Recent Studies on Strawberries. IntechOpen.
Rashid, I. A., Abdelghany, R. E., & Abd-El Hamed, W. F. (2022). Potential of bioagents application before harvest on strawberry fruit rots and quality under storage conditions. Egyptian Journal of Phytopathology.
Romanazzi, G., Feliziani, E., Santini, M., & Landi, L. (2013). Effectiveness of postharvest treatment with chitosan and other resistance inducers in the control of storage decay of strawberry. Postharvest Biology and Technology, 75, 24–27.
Shehata, S. A., Abdeldaym, E. A., Ali, M. R., Mohamed, R. M., Bob, R. I., & Abdelgawad, K. F. (2020). Effect of some citrus essential oils on postharvest shelf life and physicochemical quality of strawberries during cold storage. Agronomy, 10(10), 1466. https://doi.org/10.3390/agronomy10101466
Spadaro, D., Banani, H., Santoro, K., Garibaldi, A., & Gullino, M. L. (2017). Essential oils to control postharvest diseases of apples and peaches: Elucidation of the mechanism of action. Acta Horticulturae, 1323, 35–42.
Wonglom, P., Daengsuwan, W., Ito, S. I., & Sunpapao, A. (2019). Biological control of Sclerotium fruit rot of snake fruit and stem rot of lettuce by Trichoderma sp. T76-12/2 and the mechanisms involved. Physiological and Molecular Plant Pathology, 107, 1–7.
Yassin, M. T., Mostafa, A. A. F., & Al-Askar, A. A. (2022). In vitro antagonistic activity of Trichoderma spp. against fungal pathogens causing black point disease of wheat. Journal of Taibah University for Science, 16(1), 57–65.
Zoeir, H. A., El Zahaby, H. M., Ziedan, E. H., & Maswada, H. F. (2017). Effects of antifungal activity of essential oils, salts and antioxidant acids on pathogenic fungi and their application methods for controlling postharvest diseases in banana fruits. African Journal of Microbiology Research, 11(29), 1162–1170.
