EFFICACY OF GREEN SYNTHESIZED SILVER NANOPARTICLES AGAINST LATE LEAF SPOT DISEASE IN GROUNDNUT CAUSED BY PHAEOISARIOPSIS PERSONATA
DOI:
https://doi.org/10.48165/jpds.2026.21.01.02Keywords:
Groundnut, Late leaf spot, Nanoparticles, Ocimum sanctum, Phaeoisariopsis personataAbstract
Groundnut ( L.) is one of India’s most important oilseed crops, extensively cultivated under tropical and subtropical conditions. Productivity is frequently constrained by foliar fungal diseases, particularly late leaf spot (LLS)caused by . Under conducive environmental conditions such as prolonged leaf wetness, high humidity and moderate temperatures-the disease can induce defoliation, weaken haulm quality and cause pod yield losses exceeding 50–70%. Chemical fungicides remain the dominant management tool; however, issues such as environmental contamination, pathogen resistance and residue hazards justify the search for eco-friendly alternatives. Nanotechnology provides a novel opportunity for disease control due to the unique physicochemical properties of nanoparticles. This investigation focused on optimization of dose through poisoned food technique and its field efficacy
under Kharif conditions at Central Campus, MPKV Rahuri. Biosynthesized AgNPs exhibited reddish-brown coloration with UV-Vis absorption around 430–450 nm, indicative of spherical crystalline structures. Transmission Electron Microscopy (TEM) revealed particle sizes predominantly ranging between 20–60 nm. assays demonstrated significant mycelial inhibition at 100, 125 and 150 ppm
concentrations. Field application of 150 ppm AgNPs recorded the lowest disease severity and was statistically comparable to mancozeb and carbendazim. These findings highlight the potential of green nanomaterials as safer alternatives to fungicidesfor sustainable groundnut production.
References
Allahverdiyev, A. M., Abamor, E. S., Bagirova, M., & Rafailovich, M. (2011). Antimicrobial effects of silver nanoparticles. Future Microbiology, 6(8), 933–940.
Archana, K. J., Anusuya, D., Madhuri, R. J., & Kavitha, S. (2020). Green synthesis of silver nanoparticles and evaluation of their antifungal potential against plant pathogens. Journal of Plant Pathology & Microbiology, 11(5), 516.
Babar, M., Ijaz, S., Haq, I. U., & Khan, I. (2022). Morpho-functional characterization, in vitro study and tripartite interaction assay evaluate the potential of biosynthesized silver nanoparticles to manage Cercospora leaf spot disease in Vigna radiata. Journal of Plant Pathology, 104, 1371–1381.
Barnett, H. L., & Hunter, B. B. (1972). Illustrated Genera of Imperfect Fungi. Burgess Publishing Company, Minnesota.
Bindhani, B. K., & Panigrahi, A. K. (2015). Biosynthesis and characterization of silver nanoparticles by using leaf extracts of Ocimum sanctum L. and study of its antibacterial activities. Journal of Nanomedicine & Nanotechnology, S6–S8.
Biswas, S., & Singh, N. P. (2005). Fungicidal management of foliar diseases of groundnut in Tripura. Indian Phytopathology, 58(4), 500–502.
Chen, J., Wu, L., Lu, M., Lu, S., Li, Z., & Ding, W. (2020). Comparative study on the fungicidal activity of metallic MgO nanoparticles and macroscale MgO against soil-borne fungal phytopathogens. Frontiers in Microbiology, 11, 365.
Elgorban, A. M., Aref, S. M., Seham, S. S. M., Elhindi, K. M., Bahkali, A. H., Sayed, S. R., & M. M. A. (2016). Extracellular synthesis of silver nanoparticles using Aspergillus versicolor and evaluation of their activity on plant pathogenic fungi. Mycosphere, 7, 844–852.
Gadhiya, K. K., Kapadiya, H. J., Akbari, L. F., & Hadavani, J. K. (2018). Evaluation of fungicides for management of early leaf and late leaf spot of groundnut. International Journal of Chemical Studies, 6(3), 2718–2721.
Ijaz, M. (2011). Epidemiology and management of Cercospora leaf spot of peanut (Arachis hypogaea L.) in Punjab (M.Sc. Thesis). Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
Jacobi, W. R., & Backman, P. A. (1995). Late leaf spot and rust of peanut. In D. M. Porter, D. H. Smith, & R. Rodriguez-Kabana (Eds.), Compendium of Peanut Diseases (2nd ed.). APS Press, St. Paul, MN.
Jahagirdar, S., & Hundekar, A. R. (2010). Management strategies against frog-eye leaf spot pathogen (Cercospora nicotianae) in India. Indian Phytopathology, 63(1), 63–65.
Jo, Y. K., Kim, B. H., & Jung, G. (2009). Antifungal activity of silver ions and nanoparticles on phytopathogenic fungi. Plant Disease, 93(10), 1037–1043.
Joshi, M. S., Borkar, P. G., & Mandokhot, A. M. (2000). Bio-efficacy of carbendazim and mancozeb-based fungicide in control of early and late leaf spots of groundnut. International Arachis Newsletter, 20, 53–54.
Kim, S. W., Jung, J. H., Lamsal, K., Sock, K. Y., Min, J. S., & Lee, Y. S. (2012). Antifungal effects of silver nanoparticles (AgNPs) against various plant pathogenic fungi. Mycobiology, 40(1), 53–58.
Kokalis-Burelle, N., Porter, D. M., Rodriguez-Kabana, R., Smith, D. H., & Subrahmanyam, P. (1997). Compendium of Peanut Diseases (2nd ed.). American Phytopathological Society, USA.
Mahawar, H., Prasanna, R., Gogoi, R., Singh, S. B., Chawla, G., & Kumar, A. (2020). Synergistic effects of silver nanoparticles augmented Calothrix elenkinii for enhanced biocontrol efficacy against blight challenged tomato Alternaria plants. Biotech, 10, 102.
McDonald, D., Subrahmanyam, P., Gibbons, R. W., & Smith, D. H. (1985). Early and Late Leaf Spots of Groundnut. ICRISAT Information Bulletin No. 21. ICRISAT, Patancheru, India.
Miguel, A., Aguilar, M., Martin, M. E. S., Ortega, A. L., Cobian, P. G. E., & Sanchez, E. (2011). Synthesis and characterization of silver nanoparticles: Effect on phytopathogen Colletotrichum. Journal of Nanoparticle Research, 13, 2525–2532.
Morones, J. R., Elechiguerra, J. L., Camacho, A., Holt, K., Kouri, J. B., Ramirez, J. T., & Yacaman, M. J. (2005). The bactericidal effect of silver nanoparticles. Nanotechnology, 16, 2346–2353.
Mushrif, S. K., Manju, M. J., Shankarappa, T. H., & Nagaraju. (2017). Comparative efficacy of fungicides against tikka disease of groundnut caused by Cercospora arachidicola and Cercosporidium personatum. International Journal of Environmental Sciences, 11(1–2), 67–71.
Nath, V. R., & Kulkarni, L. G. (1967). Effect of different dates of sowing of groundnut on the development and intensity of leaf spot disease by Cercospora spp. Indian Journal of Agricultural Sciences, 37, 362–368.
Ouda, S. M. (2014). Antifungal activity of silver and copper nanoparticles on two plant pathogens, Alternaria alternata and Botrytis cinerea. Research Journal of Microbiology, 9(1), 34–39.
Panigrahi, T. (2013). Synthesis and characterization of silver nanoparticles using leaf extract of Azadirachta indica (Master's Thesis). National Institute of Technology, Rourkela, India.
Porter, D. M., Smith, D. H., & Rodriguez-Kabana, R. (1982). Peanut diseases. In H. E. Pattee & C. T. Young (Eds.), Peanut: Science and Technology (pp. 326–410). American Peanut Research and Education Society, Yoakum, Texas.
Reshi, N. A., Khan, M. A., Ahmad, M., & Dar, G. H. (2001). Effect of fungicides and their application methods on leaf spot disease and some agronomic traits in zinnia. Applied Biological Research, 3, 15–18.
Rout, Y., Behera, S., Ojha, A. K., & Nayak, P. L. (2012). Green synthesis of silver nanoparticles using Ocimum sanctum (Tulsi) and study of their antibacterial and antifungal activities. Journal of Microbiology and Antimicrobials, 4(6), 103–109.
Shedage, Y. G., Shinde, V. S., Latake, S. B., Bhalerao, B. M., & Deshmukh, S. J. (2025). Efficacy of green synthesized silver nanoparticles against late leaf spot disease in groundnut caused by Phaeoisariopsis personata. Journal of Plant Disease Sciences, 21(1), 9–14. https://doi.org/10.48165/jpds.2026.21.01.02
Singhal, A., Sainger, P. A., Srivastava, P., Soni, R., & Singh, M. (2011). Biosynthesis of silver nanoparticles using Ocimum sanctum extract and screening its antimicrobial activity. Journal of Nanoparticle Research, 13(7), 2981–2988.
Subrahmanyam, P., & McDonald, D. (1986). Diseases of groundnut in India. In Proceedings of the Workshop-cum-Seminar on High Yielding Technology for Rabi/Summer Oilseeds. Directorate of Oilseeds Research, Rajendranagar, Hyderabad.
Subrahmanyam, P., McDonald, D., Waliyar, F., Reddy, L. J., Nigam, S. N., Gibbons, R. W., Rao, V. R., Singh, A. K., Pande, S., Reddy, P. M., & Rao, P. S. (1995). Screening methods and sources of resistance to rust and late leaf spot of groundnut. ICRISAT Information Bulletin No. 47. ICRISAT, Patancheru, Andhra Pradesh, India.
Subramanyam, M., & Johnson, K. (2009). Pseudomonas fluorescens for the management of late leaf spot in groundnut. Indian Journal of Plant Protection, 37(1), 161–165.
Vincent, J. M. (1947). Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 159, 850.
Woodroof, N. C. (1933). Two leaf spots of the peanut (Arachis hypogaea L.). Journal of Phytopathology, 23, 627–640.
