Moricandia sinaica plants were obtained from South West- ern Sinai fromBotany and Microbiology Department, Fac- ulty of Science (Boy’s Branch), Al-Azhar University, Cairo, Egypt). Surface sterilization of siliques was carried out by washing in soap for 2 min, then washed under tap water for 15 min. Subsequently, dipping in Clorox (30% containing

5% sodium hypochlorite) for 15 minwith Shake vigorously, followed by three rinses in sterile distilled water. Morican- dia sinaica plantlets were propagated in vitro by seeds in siliques cultured on (3/4 strength MS medium supple- mented with 0.2 mg/lBAP + 0.1 mg/lKIN+30 g/l sugar+ 6 g/l agar at pH 5.8 and autoclaving at 1.5bar and121°C for 18 minutes). The cultures jars were incubated in a growth chamber at 25 ± 2°c under photoperiod 16h of 3000 lux intensityas Fig. (1). Also, used different strengths of MS medium (Murashige and Skoog, 1962) as (full strengths,

3/4 and 1/2 strength MS medium), also study the influence of addition growth regulator as0.2mg/l BAP + 0.2mg/l KIN to different strengths MS medium (full strengths, 3/4 and 1/2 strength MS medium). Added 30 g/l sugar+ 6 g/l agar at pH 5.8 and autoclaving at 1.5bar and 121°C for 18 min- utes to all media. All media were studied to choose the best medium to propagate Moricandia sinaica plantlets. After choose the best media and propagated, the plantlets were exposed to different doses of gamma radiation.

Gamma irradiation was carried out with 60Co source at the dose rate 0.709 KGy /h at National Centre for Radia- tion Research and Technology, Cairo, Egypt. Moricandia plantlets were exposed to gamma radiation doses of 10, 20, 30, 40,50 and 60 Gy. Then buds were cultivated on culture medium (1/2 MS medium + 6 g/l agar + 3% sucrose at pH

5.8 and autoclaving at 1.5bar and 121°C for 18 minutes).

Total genomic DNA was isolated from seven treatments of plantlets (Control,10, 20, 30, 40, 50 and 60Gy). The plant- lets samples, were weighing approximately 0.5g then were ground into a fine powder using liquid nitrogen according to Anderson et al. (1992), to enhance the DNA’s quality: two consecutive extractions were performed using a mix- ture of phenol and chloroform (1:1), followed by an ad- ditional wash with 97% ethanol (kept at −20 °C for one hour) and 70% pre-cooled ethanol, respectively. The yield and quality of the DNA were evaluated using a

spectrophotometer and gel electrophoresis. For the analy- sis of genetic markers, 20 ISSR primers.

20 different ISSR primers are G-02, G-03, CCA, CGA, GT, AG, CT, CA, ACA, ISSR2, ISSR7, ISSR10, ISSR11, ISSR12, ISSR13, ISSR14, ISSR16, ISSR18, ISSR24 and G-01 which

have 11, 14, 15, 16, 17, 18 and 19 nucleotides based on di-

or tri- nucleotide ISSR repeats as Table (1). Amplification reactions were performed in a 25 μl volume, containing:

12.5 μl 2×EasyTaq® PCR SuperMix (Lot#Q20527 ,1.25ml), 1 μl primer, 1μlof genomic DNA and 9.5 μl sterile distilled water. The thermocycling program utilized was as follows: an initial cycle at 94 °C for2 min.Then amplified for 40 cy- cles consisting of the 30s at 94°C, 30s at 41.5°C and 2 min. at 72 °C followed by 5 min. incubation at 72 °C. Amplifi- cation products were separated by gel electrophoresis on 0.8% agarose and visualized under UV illumination after staining with ethidium bromide and photographed.

The sizes of ISSR fragments were estimated by comparing the induced bands with the BERUS (100bp) DNA Ladder (WF-WF10407001). ISSR fingerprints were recorded in the binary form (1 = presence of a band and 0 = absence of a band). The percentage of polymorphic fragments in Moricandia were used to evaluate the genetic diversity be- tween control and irradiated treatments. Fragment sizes of ISSR were determined with EgyGene GelAnalyzer4 soft- ware (Ahmed, 2021). Comparison with the DNA marker. Amplified products were scored as present (1) or absent

(0) to form a binary matrix. In order to measure the infor- mativeness of the markers to differentiate between treat- ments, Total amplified band (TAB), Polymorphic band no. (PBN), % polymorphic band (%PB), Band size/bp (BS/bp), Marker index (MI), Polymorphism information content

(PIC), Heterozygosity index (H), Effective multiplex ratio (E), Arithmatic mean of H (H.av), Discriminating power (D), Resolving power (R). were calculated by the usage of

iMEC: Online Marker Efficiency Calculator, (https://ir- scope.shinyapps.io/iMEC/, Amiryousefi et al., 2018).

Table 1. Amplification results generated by ISSR primers in Moricandia sinaica plantlets.

Dried 2grams Moricandia sinaica plantlets of treatments (0, 10, 20, 30 and 40Gy) in the shade were grounded to powder. The crude extraction of dried parts of Moricandia sinaica was obtained by transferring the powder to 10 ml clean tubes and added 5 ml methanol (Sigma-Aldrich, for HPLC ≥ 99.9%) overnight.

The chemical composition samples of treatments (0, 10, 20, 30 and 40 Gy) performed using GC-TSQ mass spec- trometer (Thermo Scientific, Austin, TX, USA) with a di- rect capillary column TG–5MS (30 m x 0.25 mm x 0.25 µm film thickness). The column oven temperature was ini- tially held at 60°C and then increased by 5°C /min to 250°C withhold 2 min then increased to 300 with 30 C/min. The injector temperature was kept at 270°C. Helium was used

as a carrier gas at a constant flow rate of 1 ml/min. The solvent delay was 4 min and diluted samples of 1 µl were injected automatically using Autosampler AS3000 coupled with GC in the split mode. EI mass spectra were collected at 70 eV ionization voltages over the range of m/z 50–650 in full scan mode. The ion source and transfer line were set at 200 °C and 280 °C respectively. The components were identified by comparison of their mass spectra with those of WILEY 09 and NIST14 mass spectral database (Abd El- Kareem et al., 2016).

The data were statistically analyzed using ANOVA analy- sis to determine the level of significant differences between means as compared to the control at P≤ 0.05 level of signif- icance. The statistical software Costat (http://www.cohort. com/costat.html) was used for all analyses.

In Vitro Culture

Tissue culture technique is used in a large scale for eco- nomically programs to produce a healthy plant, and used in the production of secondary metabolites and phytocon- stituents. Plant tissue culture used for a wide range with various applications in research and industry. In the pres- ent study detected the effects of different strengths (full, 3/4 and1/2) of MS media and influence of addition growth regulator as 0.2mg/l BAP + 0.2mg/l KIN on in vitro propa- gation of Moricandia sinaica. Seedlings derived from seeds in siliques germinated directly on (3/4 strength MS medi- um supplemented with 0.2 mg/lBAP + 0.1 mg/lKIN. Then, Moricandia sinaica plantlets propagated in vitro on differ- ent strength MS medium. Data in Table (2) and Fig. (2) were Demonstrated the effect of addition 0.2 mg/l of BAP and 0.2 mg/l of KIN to different strength MS medium.The medium No. (3) (1/2 MS medium without hormones) was the best media compared to another media.Medium no. 3 had positively effect on the growth of buds, with bud sur- vival of 96.66% and the mean shoot length of 2.1 cm. The lowest growth was recorded on half strength MS medium No. (4) supplemented with 0.2 mg/l of BAP and 0.2 mg/l of KIN. Itrecorded the lowest bud survival percentage 70% and mean shoot length of 1.4 cm. Therefore, the propaga- tion of Moricandia sinaica is possible in vitro by planlets on

½ strength MS medium.

The need for medicinal plants is increasing therefore; it is necessary to find other ways to produce the amount of plant material that is needed. Propagation through tissue culture offers a

viable alternative for normal propagation because it can also be used as a complimentary strategy for conservation and utilization of genetic resources. Further, in vitro plant

regeneration through

vegetative parts is an easy and economical way for obtain- ing a large number of consistently uniform and truetotype plants within a short span of time (Abdalla et al., 2022). This study clear that the ½ MS medium (half strength MS medium without hormones) could be the beneficial effects with optimum growth for Moricandia sinaica plantlets. Similar results were obtained by Ray et al. (2006) who found that the ½ MS basal medium was the best elongation shoots of Cordyline terminalis (L.) and Stamenković et al., (2012) described that the maximum multiplication rate was completed with ½ MS medium on Campanula vele- bitica Borbás. Furthermore, depending on plant species, a diluted tissue culture medium could be used with opti- mal growth (Noor El-Deen and Abdul-Moneem, 2025). In addition, media composition is one of the furthermost critical features inducing plant growth (Rezali et al., 2017; Tütüncü et al., 2019). Thus, reducing the strength of MS media could increase total chlorophyll (chlorophyll a and chlorophyll b concentration), improving germination rates, promoting more vigorous root and shoot growth, and preventing nutrient toxicity Since MS media con- tains all the macronutrients, micronutrients and vitamin, changing the strength or concentration of MS media may alter the nutrients component. Therefore, the effect of dif- ferent medium strength could also affect the plant organo- genesis in vitro. The amount of nutrients in culture media for successful culture may vary for different plant species and genotype. A study by Mukherjee et al. (2010) proved that shoot proliferation of grape rootstock was increased when cultured on MS with half

of nitrate medium. Other than that, Fakhrul et al. (2014) stated that macronutrients manipulation in MS media could affect the growth of Stevia rebaudiana.

Table 2. Responses of Moricandia sinaica explants to different culture media (30buds/media).

Effect of Gamma Irradiation on in vitro growth ionizing radiation successfully used for medicinal and aromatic plants, which are important sources of plant secondary metabolites. Determination of optimum dose, radio sen- sitivity and treatment conditions are most vital for genetic manipulation through the induction of mutation. In this investigation, Moricandia sinaica plantlets were irradiated with gamma irradiation doses of 10, 20, 30, 40, 50 and 60 Gy). The results as in Table (3) and Fig (3) cited that low doses of gamma radiation caused significantly decreasing in survival percentage and another morphological param- eter by increase doses. Furthermore, the mortality of ex- plants was increased by increase doses. The dose of 10 Gy

caused decreasing in bud survival percentage to about 12% and decreased mean shoot length to 0.81 cm. The usage of dose 20 Gy was induced mortality in bud culture of about 16%. Also, the mean shoot length was affected negatively with the same dose to 1.14 cm. Furthermore, the mortal- ity was increased with the dose of 30, 40 and 50 Gy to 36, 64 and 76% respectively, similarly the mean shoot length decreased to 1.03, 1.23 and 1.70cm. The severe depression in survival percentage to 84%, and the shoot length was decreased to 2.08 cm with the dose of 60 Gy. These results are agreement with Awad et al. (2022) who stated the pres- ence of differences in morphological and growth param- eters between un-irradiated and irradiated treatments. The growth of plants irradiated with a high dose of gamma rays (50 Gy) was significantly inhibited.

The reasons of inhibition plant growth and development were by direct and indirect effects of gamma radiation.

Also, photosynthetic capacity was reduced by deformation of the thylakoid structure in chloroplast. So, the energy induced from photosynthesis which required for com- pleting the plant life cycle which reduced. The reduction in photosynthetic efficiency has harmful effects on plant

growth, development, and reproduction. Above all, the re- production of cells was negatively affected by high doses of gamma radiation by decreased mitosis and meiosis divi- sions. So, these effects agree with Gaafar et al. (2017), who clarified the decrease in shoot and root lengths at higher gamma radiation doses on the basis of reduction in the mi- totic activity of plant tissues.

Table 3. Effect of gamma radiation doses on some morphological parameters in Moricandia sinaica plantlets(25 plantlets/ treatments).

· Values followed by different letters are significantly difference at = 0.05 level0

· + = Formation of roots - = Deformation of roots

Several DNA-based markers are now available that can be used to accurately measure genetic diversity in plant species. ISSR markers employ primers based on a repeat sequence, amplifying the sequence between two microsat- ellites producing several amplification products per prim- er, which means they are reproducible and cost effective. The genetic diversity between irradiated Morcindia sinica plantlets with different gamma radiation doses 0, 10, 20,

30, 40, 50 and 60 Gy is summarized in Table (4).A total of 149 bands were generated from 20 ISSR primers (Fig.

4) and all of them are polymorphic bands by a range of 0 to 3 bands per primer with an average of 1.3 fragments per primer. The highest number of bands 11 observed by G-03, while the lowest number was 4 by ISSR 16 with an average of 7.45 bands per primer. The polymorphism per- centage varied from 33.33% to 72.7%, with the mean of polymorphism 58.55%. The size of the amplified products ranged from 104 bp (CA) to 1989 bp (ACA). The value of polymorphic information content (PIC) was varied from 0.2391(ISSR- 7) to 0.57 (ISSR-2) with an average 0.363 (PIC<0.5) classifying most primers as moderately infor- mative (Yehia et al., 2020). Primers G-02, CCA, CGA, GT, AG, CA, ISSR 11, ISSR 12 and ISSR 18 recorded the high- est PIC (0.371–0.374), confirming their usefulness in de-

tecting genomic variation induced by gamma rays.Marker index value (MI) was varied between 0.0176 (G-03) and 0.6449 (ISSR- 2) by an average 0.0681. Nei’s gene diversity values (H) ranged from 0.227 (ISSR- 7) to 0.6449 (ISSR-

2) indicating substantial allelic variation across loci were revealed high genetic diversity, consistent with mutagen induced base substitutions, deletions, or insertions (Mu- dibu et al., 2012). The effective multiplex ratio (E) fluctu- ated from1.000 (ISSR- 2) to 5.8333 (ISSR 7). High E values point to primers capable of amplifying several informative loci simultaneously, which improves detection efficiency. Equally, the arithmetic mean heterozygosity (H.av) values were 0.0062 (G-03) to 0.0145 (ISSR- 16), signifying that while many polymorphisms existed, they were distributed across a relatively small subset of loci, a characteristic fea- ture of targeted mutagenesis. The discriminating power

(D) was higher ranged from 0.3089 (ISSR 7) to 0.8410(G-

03) which indicating strong genotype differentiation. Also, resolving power (R) varied from 2.3333(ISSR 7) to 6.7500 (ISSR- 16). High R values indicated primers with strong diagnostic value in mutation breeding programs. ISSR (In- ter-Simple Sequence Repeats) are semi-arbitrary markers that can be amplified using PCR with one primer that par- allels to a target microsatellite. Additionally, that their am- plification does not need information about the genome

sequence and revealed higher polymorphism level than RAPD and AFLP markers according to Pandotra et al. (2013). The ISSR markers have been demonstrated to be a rapid, simple, dependable, and highly informative method for DNA fingerprinting also ISSR markers afford more truthful genetic polymorphism results so, it used to evalu- ation genetic diversity, genetic stability, genetic character- ization and identification and screening of mutant plants. The impact of g-radiation by increase radiation dose which Changes in the structure of DNA, such as breaks, trans- positions, deletions, etc., can lead to the formation of new bands (unique bands) and the removal of existing bands (polymorphic bands). So ISSR analysis showing genetic variation among untreated and irradiated treated plants will be useful in distinguishing variants with differences in morphological characteristics. In the same line with El-Gazzar et al. (2023) found that gamma radiation led to the appearance of new bands and the absence of oth- ers compared to the control plants. Disappearance of ISSR

bands in treated plantlets could be mentioned to damages of DNA as single or double-strand breaks, modified bases, oxidized bases, and bulky adducts. Similarly, DNA protein cross links, point mutations and rearrangement of chro- mosomes induced by gamma irradiation (Ginchner et al., 2008 and Abhay et al., 2014). Besides, it has been revealed that free radicals interact with biomolecules as DNA and eliminate electrons from them, which harm to both struc- ture and activity of the DNA. Though, the appearance of new bands could be correlated to the effect of mutation in- stead of DNA damage. Treated plants with highest doses of gamma rays caused in the highest reduction in content of genomic DNA. Similarly, the appearance or disappearance of bands under gamma irradiation might be considered as molecular markers for radiation processes (Hussein, 2012). It has been exhibited that effects of gamma-rays on ISSR fingerprinting might be connected to structural re- arrangements in DNA caused by different types of DNA damages (Mejri et al., 2012).

Table 4. Amplification results generated by ISSR primers in Moricandia sinaica plantlets.

· Note: Total amplified band (TAB), Polymorphic band no. (PBN), % polymorphic band (%PB), Band size/bp (BS/bp), Polymorphism information content (PIC), Marker index (MI), Heterozygosity index (H), Effective multiplex ratio (E), Arithmatic mean of H (H.av), Discriminating power (D), Resolving power (R)

Moricandia sinaica is one of xerophytic plants in Egypt. It used in traditional medicine and improve certain dis- orders such as syphilis, exhibits analgesic, anti-inflam- matory, antipyretic, antioxidant, anticancer and antigen toxic properties. However, it has many biologically active components. In this investigation, focused on organo- sulfur compounds were institute in Moricandia sinaica plantlets. Furthermore, study the effect of gamma radia- tion doses (0,10,20,30 and 40 Gy) by GC- MS. Data in Table 5 resume the result of gamma irradiation on pres- ence or disappearance of some organosulfur compounds. The GC-MS analysis presented that there are13 bioactive organosulfur compounds in both irradiated and non-ir- radiated samples were recorded. Some compounds being synthesized and others degraded depending on the impact of irradiation. Gamma radiation doses (10, 20, 30 and 40 Gy) have a negative effect on Thiocyanic acid octyl ester, MF: (C9H17NS) and 2-[3-Cyclohexylaminopropylamino] ethylthiophosphate, MF: (C11H25N2O3PS) which disap- peared from all irradiated treatments and appear in control only. While the compound 2-Aminoethanethiol hydrogen sulfate (Ester), MF: (C2H7NO3S2) were appeared in all treatments. Dose 40 Gy Participate with control in two compounds are ç-Thionodecalactone,MF: (C10H18OS) and 5à-androstan-16- one cyclic ethylene mercaptole , MF: (C21H34S2),with the proportion of these two compounds in dose 40 Gy being higher than the control. The com- pound 2-Myristynoyl pantetheine, MF: (C25H44N2O5S) appeared in all treatments except dose 20 Gy with higher proportion than the control. Three compounds tert-Hexa- decanethiol, MF:C16H34S, D-fructose, diethyl mercap- tal penta acetate, MF: C20H32O10S2 and Cefazolin, MF: C14H14N8O4S3 were appeared with dose 30Gy only. Moreover, another three compounds Leukotriene D4 methyl ester, MF:C26H42N2O6S,Benzo(B)thiophene,2- Ethyl-5,7-dimethyl, MF: C12H14S and 1-(Decylsulfonyl)- 1-Deoxyd-Mannitol ,MF: C16H34O7S respectively were disappeared from control and appeared in doses (10, 20 and 40 Gy) respectively. The influence of gamma irradia- tion on Moricandia sinaica plantlets organosulfur com- pounds rehabilitated the composition and the presence of existing substances has pharmacological characteristics, the investigation revealed that, 13 bioactive compounds in both irradiated and non-irradiated samples. So, the data shows that gamma irradiation alters the composition of or- ganosulfur compounds in Moricandia sinaica for required plant growth. In this paper, Table (6) showed some biolog- ical and chemical properties of organosulfer compounds in Moricandia sinaica which antioxidant, anti-inflamma- tory , antifungal, antibacterial and insecticidal activities. Also, the compound D-Fructose, diethyl mercaptal used as A natural sugar used as a sweetener and in food applica- tions. Moreover, the 2- [3-Cyclohexylaminopropyl amino]

ethylthiophosphate used as a radioprotective agent and detoxifier of platinum-based chemotherapy agents that helps reduce damage from ionizing radiation. Moricandia sinaica possess valuable phytochemicals as an effective and safe antiangiogenic drugs to be used as a single ther- apy or in recipe with other chemotherapy agents to treat cancer patients and remedy the complications in diabetic retinopathy, in harmony with Farooq et al. (2020). Sulfur- containing compounds (SCCs) are pivotal secondary me- tabolites widely distributed in plants, particularly within the Brassicaceae family. Sulfur (S), is considered to be the fourth greatest central nutrient element after nitrogen (N), phosphorus (P), and potassium (K). It plays an important role in the process of plant resistance to biotic and abiot- ic stress in plants. So, is one of the essential elements for growth, photosynthesis, respiration and the formation of cell membrane structures in plants. However, is a com- ponent of amino acids, chloroplasts, sulfatides, vitamins, coenzymes, and prosthetic groups (iron–S clusters, lipoic acid, thiamine and coenzyme A (Yoshimoto and Saito, 2019; Nakaiand Maruyama-Nakashita, 2020 and Lee et al., 2025). Because animals cannot synthesize S-contain- ing amino acids and plants are sources of S-compounds as Brassicaceae family vegetables such as broccoli, cabbage, and cauliflower. These vegetables contain characteristic sulfur-containing glucosinolate compounds, which are precursors to bioactive molecules like isothiocyanates and sulforaphane. as well as Liliaceae, like garlic and onion are rich in various forms of sulfur, including sulfides, thiosul- fates, and sulfoxides. As a result of their biological func- tions, such as antioxidant, anti-inflammatory, biocidal, antimicrobial, cytotoxic, a lower risk of developing several types of cancer, neurological disorders like Alzheimer’s, and reduced inflammatory bowel disease, mastitis, pho- toaging, diabetes, cardiovascular disorders, fatty liver dis- ease, and obesity (Capaldi et al., 2015; Hasanuzzaman et al., 2018; Francioso et al., 2020). Plants in the Bras- sicaceae family produce numerous sulfur-containing sec- ondary metabolites, found as a Glutathione (reduced form GSH; oxidized form GSSG) is the major non-protein thiol in plants. It plays a role as a non-enzymatic antioxidant in the ascorbate-glutathione cycle, and participates in many detoxification reactions in plants. Furthermore, GSH is also known as a central regulator of plant signaling dur- ing plant–pathogen interactions (Künstler et al., 2020). Glutathione is formed by the combination of γ-glutamate and cysteine via γ-glutamylcysteine synthetase (GSH1) through peptide bond and at the same time is combined with glycine catalyzed by glutathione synthase catalyzed by (GSH2) (Noctor et al., 2012). Both GSH1 and GSH2 are coded by a single gene and have been observed in Ara- bidopsis spp. Any alteration in one of the two genes can undergo mutations. GSH biosynthesis is affected by many factors such as sulfur availability and GSH1 activity. Genes

such as GSTM1 and GSTP1 and many more are respon- sible for the regulation of glutathione metabolism, as these genes produce enzymes that help to maintain homeostasis by glutathione recycling in plants (Hyman, 2010).Gam- ma radiation can alteration in composition of the general metabolic and various biomolecules as sulfur-containing compounds, as a part of biochemical changes caused by the induced stress. In addition to producing both sin- glestrand DNA breaks (SSBs) and double-strand DNA

breaks (DSBs), intrinsic DNA damage may include the loss of a base to form an abasic site, chemical modification of a base to form a miscoding or noncoding lesion, and sugar- phosphate backbone breakage. The accumulation of such damages (unrecognized and unrepaired DNA damage) may cause lethal mutations which in turn can reduce plant genome stability, growth, and productivity and also threat- en the organism’s immediate survival (Tuteja et al., 2001).

Table 5. Gamma radiation effect on organosulfur compounds in unirradiated and irradiated Moricandia sinaica plantlets.

Compounds Cont. 10Gy 20Gy 30Gy 40Gy

Compound name M.F.

RT MF

RT MF

RT MF

RT MF

RT MF

Ethanimidothioicacid,2 (dimethylamin) N[(methylamino carbonyl] oxy]-2-oxo-,methyl ester

C7H13N3O3S 219 5.29 741 2.36 8.33 704 1.24 4.62 633 0.65 5.13 624 1.45 4.74 719 15.53

2 Thiocyanic acid, octyl ester C9H17NS 171 8.81 650 0.21 ---- ---- ---- ---- ---- ---- ---- ---- ---- ---- ---- ----

3 2-Aminoethanethiol C2H7NO3S2 157 17.5 744 0.43 22.44 736 1.21 9.87 664 1.21 5.69 612 2.87 6.04 661 0.34

4 ç-Thionodecalactone C10H18OS 186 16.5 688 0.26 ----- ---- ----- ----- ---- ----- ----- ----- ----- 12.81 669 0.67

5 Benzo[b]thiophene, C12H14S 190 ---- ---- ----- ----- ---- ----- 4.68 665 0.74 ----- ----- ----- ----- ----- -----

6 tert-Hexadecanethiol C16H34S 258 43.82 695 4.56

7 1-(Decylsulfonyl)-1- C16H34O7S 370 42.19 635 0.45

8 D-Fructose,diethyl C20H32O10S2 496 ---- ---- ---- ---- ---- ---- ---- ---- ---- 5.39 621 4.20 ----- ----- -----

5à-Androstan-16-

9 one,cyclic ethylene mercaptole

C21H34S2 350 34.99 713 0.31 ---- ---- ---- ---- ---- ---- ---- ---- ---- 12.81 719 0.67

10 2-Myristynoyl pantetheine C25H44N2O5S 484 24.19 731 0.34 4.81 697 0.90 ---- ---- ---- 41.61 683 1.50 28.11 744 0.91

11 Cefazolin C14H14N8O4S3 454 5.76 713 1.28

Leukotriene D4 methyl ester

C26H42N2O6S 510 22.44 710 1.21

13 2-[3-Cyclohexy laminopropy C11H25N2O3PS 296 5.39 656 1.19

Table 6. Some biological and chemical activities of organosulfer compound in Moricandia sinaica plantlets.

This study demonstrated the different doses of gamma ra- diation led toalterationsin morphological, molecular, and phytochemical as organosulfer compounds, attributed to alterations in gene expression of Moricandia sinaica plant- lets. In essence, the plant’s altered gene expression is its adaptive mechanism, allowing it to build defenses against radiation-induced damage, a principle used to develop stronger crops to resist adverse abiotic stress.

The research work was supported from National Centre for Radiation Research and Technology, Egyptian Atomic En- ergy Authority, Cairo, Egypt.