Behavioural Alterations in Wistar rats induced by Mobile Phone Auditory Stimuli in a Controlled Setting.

Authors

  • Pulwansha T Amandi Thilakarathna Department of Medical Laboratory Sciences, Faculty of Health Sciences, The Open University of Sri Lanka, Nawala, Nugegoda, Sri Lanka Author
  • Mangala Gunatilake Department of Physiology and 3Rs Centre in Laboratory Animal Science, Faculty of Medicine, University of Colombo, Sri Lanka Author
  • Mayuri G Thammitiyagodage Animal Centre, Medical Research Institute, Colombo 08, Sri Lanka Author
  • Ramani Karunakaran Animal Centre, Medical Research Institute, Colombo 08, Sri Lanka Author

DOI:

https://doi.org/10.48165/jlas.2026.9.2.4

Keywords:

Behavioral studies, ethogram, mobile phone ringing, Wistar rats

Abstract

Researchers, staff of the animal centre, students and trainers frequently use their mobile phones inside the animal centre.  Therefore, this study was designed to determine the effect of mobile phone ringtones as an auditory stimulus on the  behaviour of Wistar rats. This study was conducted at the Medical Research Institute, Colombo, Sri Lanka. Rats were  separated into a controlled area and left for 1 hour before experimentation. The initial behaviour was examined for 10  minutes as the control. Rats were exposed to mobile sample ringing from 200-850Hz for one minute and the ethograms  were recorded for 10 minutes. This procedure was followed for female and male rats (gender) and young and adult  rats (maturity) under diverse housing conditions. After the intervention, the ethogram showed increased freezing and  sleeping, and decreased walking and rearing behaviours. The study indicates that these alterations in rat behaviour upon  exposure were independent (p>0.05) of gender, maturity, or housing style (single, pair, or group of four). This study con cluded that the results of behavioural research using Wistar rats may be affected by mobile phone ringing at the animal  centre, which can induce stress or defensive-related behaviour. 

 

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References

Alexandrov, P., Pupikina, M., Adaeva, Z., & Sitnikova, E. (2023). The difference between male and female rats in terms of freezing and aversive ultrasonic vocalization in an active avoidance test. Physiologia, 3(3), 406–420. https://doi.org/10.3390/physiologia3030028

Beery, A. K., & Zucker, I. (2011). Sex bias in neuroscience and biomedical research. Neuroscience & Biobehavioral Reviews, 35(3), 565–572. https://doi.org/10.1016/j.neubiorev.2010.07.002

Casarrubea, M., Aiello, S., Di Giovanni, G., Santangelo, A., Palacino, M., & Crescimanno, G. (2019a). Combining quantitative and qualitative data in the study of feeding behavior in male Wistar rats. Frontiers in Psychology, 10, 881. https://doi.org/10.3389/fpsyg.2019.00881

Casarrubea, M., Aiello, S., Santangelo, A., Di Giovanni, G., & Crescimanno, G. (2019b). Different representation procedures originated from multivariate temporal pattern analysis of the behavioral response to pain in Wistar rats tested on a hot plate under morphine. Brain Sciences, 9(9), 233. https://doi.org/10.3390/brainsci9090233

Casarrubea, M., Leca, J.-B., Gunst, N., Aiello, S., & Crescimanno, G. (2024). Behavioral mirroring in Wistar rats investigated through temporal pattern analysis. Scientific Reports, 14(1). https://doi.org/10.1038/s41598-024-71428-w

Castelhano-Carlos, M. J., & Baumans, V. (2009). The impact of light, noise, cage cleaning and in-house transport on welfare and stress of laboratory rats. Laboratory Animals, 43(4), 311–327. https://doi.org/10.1258/la.2009.0080098

Corbani, T. L., Martin, J. E., & Healy, S. D. (2021). The impact of acute loud noise on the behavior of laboratory birds. Frontiers in Veterinary Science, 7, 607632. https://doi.org/10.3389/fvets.2020.607632

Dawkins, M. S. (2003). Behaviour as a tool in the assessment of animal welfare. Zoology, 106(4), 383–387. https://doi.org/10.1078/0944-2006-00122

Eraslan, E., Castelhano-Carlos, M. J., Amorim, L., Soares-Cunha, C., Rodrigues, A. J., & Sousa, N. (2023). Home-cage behavior is impacted by stress exposure in rats. Frontiers in Behavioral Neuroscience, 17, 1195011. https://doi.org/10.3389/fnbeh.2023.1195011

Festing, M. F. W., Overend, P., Borja, M. C., & Berdoy, M. (2016). The design of animal experiments (2nd ed., pp. 102–111). SAGE Publications.

Grieco, F., Bernstein, B. J., Biemans, B., Bikovski, L., Burnett, C. J., Cushman, J. D., van Dam, E. A., Fry, S. A., Richmond-Hacham, B., Homberg, J. R., Kas, M. J. H., Kessels, H. W., Koopmans, B., Krashes, M. J., Krishnan, V., Logan, S., Loos, M., McCann, K. E., Parduzi, Q., & Pick, C. G. (2021). Measuring behavior in the home cage: Study design, applications, challenges, and perspectives. Frontiers in Behavioral Neuroscience, 15, 735387. https://doi.org/10.3389/fnbeh.2021.735387

Koch, L., Gaese, B. H., & Nowotny, M. (2022). Strain comparison in rats differentiates strain-specific from more general correlates of noise-induced hearing loss and tinnitus. Journal of the Association for Research in Otolaryngology, 23(1), 59–73. https://doi.org/10.1007/s10162-021-00822-2

Krohn, T. C., Salling, B., & Hansen, A. K. (2011). How do rats respond to playing radio in the animal facility? Laboratory Animals, 45(3), 141–144. https://doi.org/10.1258/la.2011.010067

Lauer, A. M., May, B. J., Hao, Z. J., & Watson, J. (2009). Analysis of environmental sound levels in modern rodent housing rooms. Lab Animal, 38(5), 154–160. https://doi.org/10.1038/laban0509-154

Menegassi, T. V., de Mello, V. C., Bueno, P. C. dos S., Barbalho, S. M., Detregiachi, C. R. P., Nakamura, A., & Herculiani, C. E. (2017). Influence of noise on the behavior of female Wistar rats. World Journal of Pharmaceutical and Medical Research, 3(5), 56–61.

Mogil, J. S. (2017). Laboratory environmental factors and pain behavior: The relevance of unknown unknowns to reproducibility and translation. Lab Animal, 46(4), 136–141. https://doi.org/10.1038/laban.1223

Mukherjee, P., Roy, S., Ghosh, D., & Nandi, S. K. (2022). Role of animal models in biomedical research: A review. Laboratory Animal Research, 38(1). https://doi.org/10.1186/s42826-022-00128-1

Naqvi, F., Haider, S., Perveen, T., & Haleem, D. J. (2012). Subchronic exposure to noise affects locomotor activity and produces anxiogenic- and depressive-like behavior in rats. Pharmacological Reports, 64(1), 64–69. https://doi.org/10.1016/S1734-1140(12)70731-4

Perkins, A. E., Varlinskaya, E. I., & Deak, T. (2019). Impact of housing conditions on social behavior, neuroimmune markers, and oxytocin receptor expression in aged male and female Fischer 344 rats. Experimental Gerontology, 123, 24–33. https://doi.org/10.1016/j.exger.2019.05.005

Robinson, N. B., Krieger, K., Khan, F. M., Huffman, W., Chang, M., Naik, A., Yongle, R., Hameed, I., Krieger, K., Girardi, L. N., & Gaudino, M. (2019). The current state of animal models in research: A review. International Journal of Surgery, 72, 9–13. https://doi.org/10.1016/j.ijsu.2019.10.015

Temple, D., Manteca, X., Velarde, A., & Dalmau, A. (2011). Assessment of animal welfare through behavioural parameters in Iberian pigs in intensive and extensive conditions. Applied Animal Behaviour Science, 131(1–2), 29–39. https://doi.org/10.1016/j.applanim.2011.01.013

Totty, M. S., Warren, N., Huddleston, I., Ramanathan, K. R., Ressler, R. L., Oleksiak, C. R., & Maren, S. (2021). Behavioral and brain mechanisms mediating conditioned flight behavior in rats. Scientific Reports, 11(1), 8215. https://doi.org/10.1038/s41598-021-87559-3

Vasilyeva, I. N., Bespalov, V. G., Semenov, A. L., Baranenko, D. A., & Zinkin, V. N. (2017). The effects of low-frequency noise on rats: Evidence of chromosomal aberrations in the bone marrow cells and the release of low-molecular-weight DNA in the blood plasma. Noise & Health, 19(87), 79–83. https://doi.org/10.4103/nah.NAH_39_16

Voipio, H.-M., Nevalainen, T. J., Halonen, P., Hakumäki, M., & Björk, E. (2006). Role of cage material, working style and hearing sensitivity in perception of animal care noise. Laboratory Animals, 40(4), 400–409. https://doi.org/10.1258/002367706778476334

Yoruk, M., Okkay, U., Savaş, A., Bayram, C., Sezen, S., Ertugrul, S. M., & Hacımuftoglu, A. (2022). Behavioral tests used in experimental animal models. Anatolian Journal of Biology, 2, 14–22.

Zhvania, M., Gogokhia, N., Tizabi, Y., Japaridze, N., Pochkidze, N., Lomidze, N., Rzayev, F., & Gasimov, E. (2020). Behavioral and neuroanatomical effects of exposure to white noise in rats. Neuroscience Letters, 728, 134898. https://doi.org/10.1016/j.neulet.2020.134898

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Published

2026-06-30

How to Cite

Behavioural Alterations in Wistar rats induced by Mobile Phone Auditory Stimuli in a Controlled Setting . (2026). Journal of Laboratory Animal Science, 9(2), 25-34. https://doi.org/10.48165/jlas.2026.9.2.4