Rapid and Easy Identification of Animal Species for Forensic Application Using NewMarkers Based on the Mitochondrial 16S rRNAgene

Authors

  • N Haider Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS) , PO Box 6091, Damascus, Syria.
  • Achkar WAL Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS) , PO Box 6091, Damascus, Syria.
  • Imad Nabulsi Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS) , PO Box 6091, Damascus, Syria.

DOI:

https://doi.org/10.48165/

Keywords:

Animal species, Forensic, Identification, Mitochondrial 16S, rRNA

Abstract

The availability of genetic markers for identification of humans and some animal species by analysis of DNA of these species biological  traces that can be found in crime scenes is of great importance as non-deterministic evidence in criminal investigations. Mitochondrial  DNA(mtDNA) provides forensic scientists with a valuable tool to determine the source of DNAextracted from damaged, degraded or very  small biological samples. The aim of this work was to develop a new, simple, fast and economical method based on polymerase chain  reaction- restriction fragment length polymorphism (PCR-RFLP, using Tru 1I, Hae III, Taq I, and Dra I) of the mitochondrial 16S rRNA gene (500 bp) for differentiation of human blood form the blood of 11 animals (camel, cow, sheep, goat, buffalo, pig, deer, horse, donkey,  dog and cat) and for distinction of the bloods of these animals from each other. The method applied here proved suitable for the  differentiation among the analyzed species.  

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Published

2023-12-05

Issue

Vol. 45 No. 1 (2023): Journal of Indian Academy of Forensic Medicine (Jan-Mar) 2023

Section

Articles

How to Cite

Rapid and Easy Identification of Animal Species for Forensic Application Using NewMarkers Based on the Mitochondrial 16S rRNAgene. (2023). Journal of Indian Academy of Forensic Medicine, 45(1), 7–13. https://doi.org/10.48165/