Effect of substrate variation on the nutritional composition of oyster mushroom (Pleurotus ostreatus)

Authors

  • Mallam Udayasri Department of Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India
  • Ram Chandra Department of Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India
  • Saurabh Bharti Department of Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India
  • Tulasi Korra Department of Plant Pathology, School of Agriculture, ITM University, Gwalior-474001, Madhya Pradesh, India

DOI:

https://doi.org/10.48165/jefa.2026.21.1.5

Keywords:

Mushroom, substrate, moisture content, ash content, dry matter content, crude protein.

Abstract

The present investigation assessed the influence of various agricultural substrates on nutritional composition of oyster mushrooms (Pleurotus ostreatus). Five substrate treatments were evaluated: wheat straw, paddy straw, maize straw, a mixture of wheat straw and green gram straw (2:1), and a mixture of wheat straw with tamarind leaves (2:1). The harvested fruiting bodies were analyzed for moisture content, dry matter content, ash content, and total crude protein. Moisture levels differed notably across treatments, ranging from 86.12 to 90.30 per cent, the highest value recorded was in mushrooms cultivated on wheat straw mixed with tamarind leaves. Ash content was greatest in mushrooms grown on wheat straw (9.45 g 100 g-1) and lowest produced from the wheat straw+tamarind leaves (8.05 g 100 g-1). The maximum total crude protein content (27.60%) was obtained from mushrooms grown on the mixture of wheat straw and green gram straw. Overall, the study highlighted that substrate composition had a significant impact on the nutritional composition of oyster mushrooms. Legume–based mixtures were particularly effective in improving protein content. These results provided useful guidance for selecting substrate combinations that enhance the nutritional value of oyster mushroom. 

 

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References

Abid, A. H., Hamid, A., Naz, R. M. M., Shah, S. Z. A., Anjum, S., Khan, M. T., & Ilyas, M. (2020). Impact of different lignocellulose substrates on growth and yield of oyster mushroom (Pleurotus ostreatus). Pure and Applied Biology, 9, 768–775.

Akola, R., Sapre, S., Talaviya, J. R., Lakhani, K., & Parakhia, M. V. (2024). Effect of agricultural waste on nutritional composition of oyster mushroom (Pleurotus ostreatus). Environment Conservation Journal, 25, 74–83.

Ashraf, J., Ali, M. A., Ahmad, W., Ayyub, C. M., & Shafi, J. (2013). Effect of different substrate supplements on oyster mushroom (Pleurotus spp.) production. Food Science and Technology, 1, 44–51.

Assemie, A., & Abaya, G. (2022). The effect of edible mushrooms on health and their biochemistry. International Journal of Microbiology, 2022, 8744788.

Bas, C. (1991). A short introduction to the ecology, taxonomy and nomenclature of the genus Agaricus. In Genetics and breeding of Agaricus: Proceedings of the First International Seminar on Mushroom Science (pp. 21–24).

Bernaś, E., Jaworska, G., & Lisiewska, Z. (2006). Edible mushrooms as a source of valuable nutritive constituents. Acta Scientiarum Polonorum Technologia Alimentaria, 5, 5–20.

Bijla, S. (2023). Status of mushroom production: Global and national scenario. Mushroom Research, 32.

Caglarirmak, N. (2007). The nutrients of exotic mushrooms (Lentinula edodes and Pleurotus species) and an estimated approach to the volatile compounds. Food Chemistry, 105, 1188–1194.

Chang, R. (1996). Functional properties of edible mushrooms. Nutrition Reviews, 54, S91.

Chang, S. T., & Miles, P. G. (1989). Edible mushrooms and their cultivation. CRC Press, New York, pp. 307–312.

Chang, S. T., Lau, O. W., & Cho, K. Y. (1981). The cultivation and nutritional value of Pleurotus sajor-caju. European Journal of Applied Microbiology and Biotechnology, 12, 58–62.

Choręziak, A., Rosiejka, D., Michałowska, J., & Bogdański, P. (2025). Nutritional quality, safety and environmental benefits of alternative protein sources—An overview. Nutrients, 17, 1148.

Croan, S. C. (2004). Conversion of conifer wastes into edible and medicinal mushrooms. Forest Products Journal, 54, 68–76.

Elkanah, F. A., Oke, M. A., & Adebayo, E. A. (2022). Substrate composition effect on the nutritional quality of Pleurotus ostreatus (MK751847) fruiting body. Heliyon, 8, eXXXXX.

Galanakis, C. M. (2021). Functionality of food components and emerging technologies. Foods, 10, 128.

Hoa, H. T., Wang, C. L., & Wang, C. H. (2015). The effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology, 43, 423–434.

Mattila, P., Salo-Väänänen, P., Könkö, K., Aro, H., & Jalava, T. (2002). Basic composition and amino acid contents of mushrooms cultivated in Finland. Journal of Agricultural and Food Chemistry, 50, 6419–6422.

Rahi, D. K., & Malik, D. (2016). Diversity of mushrooms and their metabolites of nutraceutical and therapeutic significance. Journal of Mycology, 2016, 7654123.

Randive, S. D. (2012). Cultivation and study of growth of oyster mushroom on different agricultural waste substrates and its nutrient analysis.

Sharma, V. P., Annepu, S. K., Gautam, Y., Singh, M., & Kamal, S. (2017). Status of mushroom production in India. Mushroom Research, 26.

Zhou, Y., Wang, D., Zhou, S., Duan, H., Guo, J., & Yan, W. (2022). Nutritional composition, health benefits, and application value of edible insects: A review. Foods, 11, 3961.

Published

2026-02-06

How to Cite

Udayasri, M., Chandra, R., Bharti, S., & Korra, T. (2026). Effect of substrate variation on the nutritional composition of oyster mushroom (Pleurotus ostreatus). Journal of Eco-Friendly Agriculture, 21(1), 17-19. https://doi.org/10.48165/jefa.2026.21.1.5