Micro-propagated Musa paradisiaca L. with Pseudomonas fluorescens revealing phytostimulatory activities to reduce the hardening duration
DOI:
https://doi.org/10.48165/jefa.2026.21.1.16Keywords:
In vitro propagation, Pseudomonas fluorescens, PGPR, inflorescence, L-tryptophan, bio-fertilizer.Abstract
Immature male flower buds of the diploid landrace Musa paradisiaca L. were collected from healthy plants in the farmlands of Ernakulam district, Kerala, between June, 2021 and June, 2023 for laboratory cultivation. The excised flower buds were cultivated on Murashige and Skoog (MS) basal media supplemented with 6-benzylaminopurine (BAP) to promote growth. Multiplication of shoots was accomplished utilising MS medium with 4 mg l-1 BAP, succeeded by elongation and roots on hormone-free MS medium. Acclimatised plantlets were relocated to a greenhouse for additional assessment of the effects of plant growth-promoting rhizobacteria (PGPR). The simultaneous application of Pseudomonas fluorescens and L-tryptophan markedly improved plant vigour, growth metrics and biomass relative to control groups. After one month, plants exhibited enhanced root and shoot length, leaf quantity, stem length and both fresh and dry weight. This underscores their promise as an efficient, sustainable biofertilizer in virus-free plantain cultivation by in vitro propagation.
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