Determination of Selected Mineral Concentrations in Sheep Wool and Their Relationship with Geographical Location: A Comparative Study of Two Regions in Northeastern Algeria
DOI:
https://doi.org/10.48165/ijapm.2026.42.03.09Keywords:
ICP-OES, Macroelements, Sheep, Trace elements, WoolAbstract
Animal health is closely linked to environmental factors such as climate and geography, which affect the quality and availability of local feed. Since nutrition has a direct impact on the composition of biological materials such as wool, it is important to understand how these conditions influence elemental concentrations. This study evaluates and compares the contents of selected chemical elements (calcium, potassium, magnesium, sodium, copper and zinc) in Ouled Djellal sheep wool collected from two Algerian regions characterized by hot arid desert (Biskra) and semi-arid (Batna) climates. Wool samples were analyzed using inductively coupled plasma optical emission spectrometry (ICP-OES), and the data were statistically evaluated using the Mann-Whitney U test. Element concentrations varied slightly between the two regions depending on the element; however, only copper significantly differed between the two regions, with markedly higher concentrations in the semi-arid region (P < 0.001). This exploratory study provides baseline data on the mineral composition of wool from the Ouled Djellal breed in northeastern Algeria and suggests that environmental factors may influence the accumulation of certain mineral elements in wool, which may serve as a non-invasive indicator in environmental and field studies. However, further research with larger sample sizes is needed to confirm its practical applicability.
References
Agradi, S., Munga, A., Barbato, O., Palme, R., Tarhan, D., Bilgiç, B., Dokuzeylül, B., Ercan, A. M., Or, M. E., Brecchia, G., Curone, G., Draghi, S., Vigo, D., Marongiu, M. L., González-Cabrera, M., & Menchetti, L. (2023). Goat hair as a bioindicator of environmental contaminants and adrenal activation during vertical transhumance. Frontiers in Veterinary Science, 10, 1274081. https://doi.org/10.3389/fvets.2023.1274081
Aydin, I. (2008). Comparison of dry, wet and microwave digestion procedures for the determination of chemical elements in wool samples in Turkey using ICP-OES technique. Microchemical Journal, 90(1), 82–87. https://doi.org/10.1016/j.microc.2008.03.011
Barbosa, F., Jr., Tanus-Santos, J. E., Gerlach, R. F., & Parsons, P. J. (2005). A critical review of biomarkers used for monitoring human exposure to lead: Advantages, limitations, and future needs. Environmental Health Perspectives, 113(12), 1669–1674. https://doi.org/10.1289/ehp.7917
Baziz, N. (2018). Contribution à la caractérisation et modélisation du cycle de l’eau potable et les risques associés dans la Wilaya de Batna (Approche par SIG) [Doctoral thesis, University of Batna 2]. http://eprints.univ-batna2.dz/1537/
Boumaraf, B., & Saadi, I. (2023). The gypsum in the soils of Ziban, Algerian Northern Sahara. Agrária Acadêmica Journal, 6(5), 76–88. https://doi.org/10.32406/v6n5/2023/76-88/agrariacad
Castrica, M., Andoni, E., Quattrone, A., Koleci, X., Ozuni, E., Zalla, P., Postoli, R., Menchetti, L., Bilgiç, B., Tarhan, D., Yalcin, I. E., Dova, I., Fehri, N. E., Or, M. E., Munga, A., Beqiraj, D., Curone, G., & Agradi, S. (2025). Livestock animal hair as an indicator of environmental heavy metals pollution in Central Albania. Animals, 15(13), 1898. https://doi.org/10.3390/ani15131898
Combs, D. K. (1987). Hair analysis as an indicator of mineral status of livestock. Journal of Animal Science, 65(6), 1753–1758. https://doi.org/10.2527/jas1987.6561753x
Draghi, S., Fehri, N. E., Ateş, F., Özsobacı, N. P., Tarhan, D., Bilgiç, B., Dokuzeylül, B., Yaramış, Ç. P., Ercan, A. M., Or, M. E., Cagnardi, P., Brecchia, G., Curone, G., & Di Cesare, F. (2024). Use of hair as matrix for trace elements biomonitoring in cattle and roe deer sharing pastures in Northern Italy. Animals, 14(15), 2209. https://doi.org/10.3390/ani14152209
El-Naser, E. M. A., Mohamed, G. A. E., & Rateb, M. H. (2013). Wool analysis as an indicator for diagnosis of some trace elements deficiency in sheep. Assiut Veterinary Medical Journal, 59(137), 120–123. https://doi.org/10.21608/avmj.2013.171509
Fageria, N. K., Baligar, V. C., & Clark, R. B. (2002). Micronutrients in crop production. In D. L. Sparks (Ed.), Advances in agronomy (Vol. 77, pp. 185–268). Academic Press. https://doi.org/10.1016/S0065-2113(02)77015-6
Fan, Q., Wang, Z., Chang, S., Peng, Z., Wanapat, M., Bowatte, S., & Hou, F. (2020). Relationship of mineral elements in sheep grazing in the highland agro-ecosystem. Asian-Australasian Journal of Animal Sciences, 33(1), 44–52. https://doi.org/10.5713/ajas.18.0955
Gaetke, L. M., Chow-Johnson, H. S., & Chow, C. K. (2014). Copper: Toxicological relevance and mechanisms. Archives of Toxicology, 88(11), 1929–1938. https://doi.org/10.1007/s00204-014-1355-y
Grace, N. D., & Sumner, R. M. W. (1986). Effect of pasture allowance, season, and breed on the mineral content and rate of mineral uptake by wool. New Zealand Journal of Agricultural Research, 29(2), 223–230. https://doi.org/10.1080/00288233.1986.10426975
Janíček, M., Massányi, M., Kováčik, A., Halo, M., Jr., Tirpák, F., Błaszczyk-Altman, M., Albrycht, M., Stawarz, R., Halo, M., & Massányi, P. (2025). Content of biogenic elements in sheep wool by the regions of Slovakia. Biological Trace Element Research, 203(4), 1886–1897. https://doi.org/10.1007/s12011-024-04328-9
Jin, X., Meng, L., Qi, Z., & Mi, L. (2024). Effects of dietary selenium deficiency and supplementation on liver in grazing sheep: Insights from transcriptomic and metabolomic analysis. Frontiers in Veterinary Science, 11, 1358975. https://doi.org/10.3389/fvets.2024.1358975
Jutha, N., Jardine, C., Schwantje, H., Mosbacher, J., Kinniburgh, D., & Kutz, S. (2022). Evaluating the use of hair as a non-invasive indicator of trace mineral status in woodland caribou (Rangifer tarandus caribou). PLOS ONE, 17(6), e0269441. https://doi.org/10.1371/journal.pone.0269441
Khazai, N., Judd, S. E., & Tangpricha, V. (2008). Calcium and vitamin D: Skeletal and extraskeletal health. Current Rheumatology Reports, 10(2), 110–117. https://doi.org/10.1007/s11926-008-0020-y
Lakhdari, S. (2022). Apport du traçage chimique et isotopique à l’identification des aquifères des Aurès [Doctoral thesis, University 8 Mai 1945 of Guelma]. https://dspace.univ-guelma.dz/xmlui/handle/123456789/1268
Lamand, M. (1978). Les oligo-éléments. Proligo.
Lee, J., Bray, A. R., & Grace, N. D. (1992). Wool growth and elemental composition. Proceedings of the New Zealand Society of Animal Production, 52, 281–283. https://www.nzsap.org/system/files/proceedings/1992/ab92072.pdf
López-Alonso, M. (2012). Trace minerals and livestock: Not too much not too little. ISRN Veterinary Science, 2012, 704825. https://doi.org/10.5402/2012/704825
Mansouri, L. M., Kheloufi, A., Djelilate, M., Mami, A., Belatreche, R., Goudja, T., Aksa, H., Abdelhamid, O., Rihane, M. R., Laourari, Z., Zeroual, I., Boukhecha, M., & Kheloufi, K. I. (2026). Diversité et disponibilité des espèces fourragères pour l’alimentation des chameaux dans la zone nord du Sahara algérien (Biskra, Algérie). Livestock Research for Rural Development, 38, Article 5. http://www.lrrd.org/lrrd38/1/3805akel.html
McDonald, P., Edwards, R. A., Greenhalgh, J. F. D., Morgan, C. A., Sinclair, L. A., & Wilkinson, R. G. (2011). Animal nutrition (7th ed.). Pearson Education Limited.
Menani, M. R. (2000). Caractérisation du système aquifère alluvionnaire de la plaine d’El Madher (Nord-Est algérien) en vue de l’expression de son bilan hydrodynamique au moyen d’un modèle de simulation en régime permanent. Bulletin de la Société Géologique d’Algérie, 11(1), 107–117. https://asjp.cerist.dz/en/article/5041
Merdaci, I. S. (2020). La modélisation de la gestion d’irrigation dans la région de Biskra en utilisant le traitement des images satellitaires (la télédétection) [Doctoral thesis, University Mohamed Khider of Biskra]. http://thesis.univ-biskra.dz/5338/
Miloudi, B., & Masmoudi, A. (2022). Effect of foliar application of micronutrients on durum wheat in salted and calcareous soil. In E. Heggy, V. Bermudez, & M. Vermeersch (Eds.), Sustainable energy-water-environment nexus in deserts (pp. 629–634). Springer. https://doi.org/10.1007/978-3-030-76081-6_79
Moniello, G., Infascelli, F., Pinna, W., & Camboni, G. (2005). Mineral requirements of dairy sheep. Italian Journal of Animal Science, 4(Suppl. 1), 63–74. https://doi.org/10.4081/ijas.2005.1s.63
Patkowska-Sokoła, B., Dobrzański, Z., Osman, K., Bodkowski, R., & Zygadlik, K. (2009). The content of chosen chemical elements in wool of sheep of different origins and breeds. Archives Animal Breeding, 52(4), 410–418. https://doi.org/10.5194/aab-52-410-2009
Peacock, M. (2010). Calcium metabolism in health and disease. Clinical Journal of the American Society of Nephrology, 5(Suppl. 1), S23–S30. https://doi.org/10.2215/CJN.05910809
Perillo, L., Arfuso, F., Piccione, G., Dara, S., Tropia, E., Cascone, G., Licitra, F., & Monteverde, V. (2021). Quantification of some heavy metals in hair of dairy cows housed in different areas from Sicily as a bioindicator of environmental exposure—A preliminary study. Animals, 11(8), 2268. https://doi.org/10.3390/ani11082268
Raab, A., Hansen, H. R., Zhuang, L., & Feldmann, J. (2002). Arsenic accumulation and speciation analysis in wool from sheep exposed to arsenosugars. Talanta, 58(1), 67–76. https://doi.org/10.1016/S0039-9140(02)00257-6
Senila, M. (2024). Recent advances in the determination of major and trace elements in plants using inductively coupled plasma optical emission spectrometry. Molecules, 29(13), 3169. https://doi.org/10.3390/molecules29133169
Shalaby, H. A., Younis, S. S. S., & Aly, M. A. M. (2010). Role of the wool analysis in diagnosis of some nutritional deficiency diseases. Assiut Veterinary Medical Journal, 56(125), 1–10. https://doi.org/10.21608/avmj.2010.173898
Stone, M. S., Martyn, L., & Weaver, C. M. (2016). Potassium intake, bioavailability, hypertension, and glucose control. Nutrients, 8(7), 444. https://doi.org/10.3390/nu8070444
Szigeti, E., Kátai, J., Komlósi, I., Oláh, J., & Szabó, C. (2020). Newly grown wool mineral content response to dietary supplementation in sheep. Animals, 10(8), 1390. https://doi.org/10.3390/ani10081390
Wang, H., Liu, Y., Qi, Z., Wang, S., Liu, S., Li, X., Wang, H., Wang, X., Xia, X., & Zhu, X. (2014). The estimation of soil trace elements distribution and soil-plant-animal continuum in relation to trace elements status of sheep in Huangcheng area of Qilian Mountain Grassland, China. Journal of Integrative Agriculture, 13(1), 140–147. https://doi.org/10.1016/S2095-3119(13)60504-3

