Histopathological Study of the Regenerative Effect of Selenium and Hydroxyapatite Nanoparticles on Proximal Tibial Growth Plate Fracture in Goats
DOI:
https://doi.org/10.48165/ijapm.2026.42.02.16Keywords:
Growth plat, Goat, Selenium, Hydroxyapatite, Tibial fractureAbstract
Growth plate fracture is very complicated fracture may associate with stunting of bone growth or bone deviation and create unique diagnostic and therapeutic challenges. The present study aimed to investigate the regenerative effect of hydroxyapatite and selenium nanoparticles on tibial growth plate injury in goats .Thirty young local goats aged (4-6) month weighing (12-16) kg were used in this study, the animals were divided randomly into three equal groups(10 for each one ) :Control, Hydroxyapatite, and selenium groups .After following aseptic techniques for preparation of the surgical site and exposure of proximal tibial growth plate , 5 mm was removed from the growth plate by using bone oscillator. In control group, the growth plate gap was filled with hydrogel directly, while in Hydroxyapatite group the gap was filled with hydroxyapatite nanoparticles - hydrogel composite at dose rate 1 mg/ml of hydrogel and filled with composite of selenium and hydrogel in selenium group at dose rate 125µg/ml. Growth plate healing process was evaluated histologically by hematoxylin and eosin(H&E) and toluidine blue staining. Histopathological finding revealed the histological scoring results demonstrate that selenium group provided the most favorable regenerative outcome, promoting cartilage regeneration and physeal restoration while effectively preventing undesirable bone bridge formation. The hydroxyapatite group showed partial improvement compared with the control group but was less effective than selenium in restoring normal growth plate architecture.
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