Comparison of Hematological, Biochemical, and Hormonal Parameters Between Fetal Sex in Different Stages of Gestation of Awassi Ewes
DOI:
https://doi.org/10.48165/ijapm.2026.42.02.10Keywords:
Herbal growth promoters, antibiotic, lymphoid organ, broilerAbstract
The physiological adaptations of pregnant ewes are influenced by gestational stage and fetal sex, which affect hematological, biochemical, and hormonal responses. However, there is limited information regarding small ruminants in practical settings. This study aimed to evaluate how fetal sex during different gestation periods impacts selected biochemical, hematological, and hormonal parameters in pregnant ewes. The study was conducted in a sheep field in Suq Al-Shuyukh, Dhi-Qar province, Iraq, from September 2025 to February 2026. The study sampled 600 pregnant ewes (aged 2-5 years, weighing 40-50 kg). Pregnancy stage and fetal sex were determined via transabdominal ultrasonography (XF50, 3.5 MHz). The ewes were classified into three gestational stages (first, second, and third trimesters) with each stage further divided by fetal sex (male and female, n=10 each). There were significant differences (P ≤ 0.05) in hematological and biochemical parameters based on fetal sex and gestational stage in ewes. In the first trimester, ewes with male fetuses displayed higher values. In the second trimester, those with female fetuses showed significant variations in hematological parameters (RBC, HGB, MCHC, and MCH). In the third trimester, the RBC count was notably higher in ewes carrying female fetuses. Additionally, fetal sex and gestational stage influenced biochemical markers, including ALT, AST, total protein, globulin, glucose, and cholesterol. Hormonal analysis indicated that placental lactogen, cortisol, estradiol (E2), and progesterone (P4) were significantly affected by these factors. In conclusion, fetal sex and gestational stage significantly influence the hematological, biochemical, and hormonal factors in pregnant ewes, making them important for reproductive assessment and herd management.
References
Abed, N. M. (2024). Protective effect of propolis extract against nickel chloride and/or carbon tetrachloride-induced alterations in physiological and endocrine functions in adult male rats. Journal of Animal Health and Production, 12(Special Issue 1), 99–106.
Abed, N. M. (2025). A comparative study on the protective effects of flaxseed oil extract and vitamin E against doxorubicin-induced cardiotoxicity and physiological alterations in male rats. Journal of Bioscience and Applied Research. https://doi.org/10.21608/jbaar.2026.432669.1334
Ajayi, F. O., Iyasele, M. N., & Onyechukwu, J. E. (2021). Analysis of genetic and non-genetic factors affecting hematology and biochemical parameters of Nigerian indigenous sheep. Nigerian Journal of Animal Science, 23, 24–38.
Al-Qaraghouli, M., & Fang, Y. M. V. (2017). Effect of fetal sex on maternal and obstetric outcomes. Frontiers in Pediatrics, 5, Article 144. https://doi.org/10.3389/fped.2017.00144
Al-Samarai, F. R., & Al-Jbory, W. A. H. (2017). Effect of environmental factors on hematological parameters in apparently healthy Iraqi Awassi sheep. Journal of Entomology and Zoology Studies, 5, 1668–1671.
Alwan, S. F. (1998). Reproductive performance and some physiological characteristics of Awassi sheep in Iraq. [Publisher details missing].
Amin, M., Alwan, S., & Ibrahim, N. (2010). Blood progesterone and estrogen hormone levels during pregnancy and after birth in Iraqi sheep and goat. Basrah Journal of Veterinary Research, 9, 153–157.
Antunović, Z., Novoselec, J., Šperanda, M., Vegara, M., Pavić, V., Mioč, B., & Djidara, M. (2011). Changes in biochemical and hematological parameters and metabolic hormones in Tsigai ewes’ blood in the first third of lactation. Archives Animal Breeding, 54(5), 535–545.
Bosc, J. M., Djiane, J., Durand, P., Pelletier, J., & Ravault, P. J. (1982). Influence of season on plasma levels of prolactin, placental lactogen hormone, and luteinizing hormone during the second half of gestation in the ewe. Reproduction Nutrition Development, 22(3), 485–493.
Clifton, V. L. (2010). Sex and the human placenta: Mediating differential strategies of fetal growth and survival. Placenta, 31(Suppl.), S33–S39.
El-Sherif, M. M. A., & Assad, F. (2001). Changes in some blood constituents of Barki ewes during pregnancy and lactation under semi-arid conditions. Small Ruminant Research, 40(3), 269–277. https://doi.org/10.1016/S0921-4488(01)00174-2
Gürgöze, S. Y., Zonturlu, A. K., Özyurtlu, N., & İçen, H. (2009). Investigation of some biochemical parameters and mineral substances during pregnancy and postpartum period in Awassi ewes. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 15(6), 957–963.
Jain, N. C. (1993). Essentials of veterinary hematology. Lea & Febiger.
Kamil, M. A. (2024). Influence of maternal and lamb factors on the level of estrogen during three physiological statuses of ewes. International Journal of Veterinary Science, 13(2), 186–190. https://doi.org/10.47278/journal.ijvs/2023.080
Kaneko, J. J., Harvey, J. W., & Bruss, M. L. (2008). Clinical biochemistry of domestic animals (6th ed.). Academic Press.
Luigi, A., Arianna, B., Deborah, L. F., Marco, Q., Rosanna, V., & Esterina, F. (2021). Steroid hormonal endpoints in goats carrying single or twin fetuses reared in semi-extensive systems. Archives Animal Breeding, 64, 467–474.
Mor, G., & Cardenas, I. (2010). The immune system in pregnancy: A unique complexity. American Journal of Reproductive Immunology, 63(6), 425–433.
Nagel, C., Aurich, C., & Aurich, J. (2019). Stress effects on the regulation of parturition in different domestic animal species. Animal Reproduction Science, 207, 153–161.
Nazifi, S., Saeb, M., & Ghavami, S. M. (2002). Serum lipid profile in Iranian fat-tailed sheep in late pregnancy, at parturition and during the post-partum period. Journal of Veterinary Medicine Series A, 49(1), 9–12.
Pereira, F. B., et al. (2015). Hematological profile of Santa Inês ewes supplemented on pasture at the last third of pregnancy and postpartum. Ciência Animal Brasileira, 16(3), 350–357. https://doi.org/10.1590/1089-6891v16i327573
Piccione, G., Caola, G., Giannetto, C., Grasso, F., Runzo, S. C., Zumbo, A., & Pennisi, P. (2009). Selected biochemical serum parameters in ewes during pregnancy, post-parturition, lactation and dry period. Animal Science Papers and Reports, 27(4), 321–330.
Probo, M., Carolif, F., Kindahl, H., Faustini, M., Galeati, G., & Veronesi, M. (2011). Peripartal hormonal changes in alpine goats: A comparison between physiological and pathological parturition. Reproduction in Domestic Animals, 46, 1004–1010.
Rook, J. S. (2000). Pregnancy toxemia of ewes, doe, and beef cows. Veterinary Clinics of North America: Food Animal Practice, 16(2), 293–316. https://doi.org/10.1016/S0749-0720(15)30107-9
Taher, J. K., Salman, R. S., Jabbar, N. K., Hussein, K. A., & Kadhim, H. J. (2026). Comparative evaluation of hormonal treatments for estrus induction and conception in anestrous postpartum dairy cows. Journal of Animal Health and Production, 14(1), 278–288. https://doi.org/10.17582/journal.jahp/2026/14.1.278.288
