Influence of Date Molasses and Honey on Reproductive Performance and Hormone Levels in Male Rabbits (Bucks)
DOI:
https://doi.org/10.48165/ijapm.2026.42.01.17Keywords:
Rabbits, FSH, Date Molasses, Honey, Luteinizing HormonesAbstract
Background: The increased curiosity in natural nutraceuticals has led to the investigations into the effects of dietary supplements on animal reproductive performance. Objective: The study aimed to determine the impact of consuming honey and date molasses on reproductive hormone levels, blood glucose and testicular histopathology in male New Zealand White rabbits. Methods: 15 male rabbits were selected at random and divided into three groups (n=5 rabbits each) as follows: the control group (G1), date molasses group (G2, 1 g/kg), and honey group (G3, 1 g/kg), which were administered orally over 45 days. We analyzed the follicle-stimulating hormone, luteinizing hormone, testosterone and blood sugar levels at 0, 15, 30 and 45 days. The testicular tissues were tested by using histological examination following the experiment. Findings: The results showed significant increases (P<0.05) in the levels of follicle-stimulating hormone, luteinizing hormone, and testosterone in G2 and G3 as compared to G1 and the strongest hormonal response was observed in the honey group. G2 and G3 significantly
elevated the level of blood glucose but the levels were normal. G1 showed a normal testicular architecture, G2 showed moderate degenerative alterations and G3 showed modest changes with retained spermatogenesis via histopathological examination. Conclusions: Honey and date molasses of honey enhanced reproductive hormone profiles but honey had better results in stimulating hormonal activity and maintaining healthier testicular tissues. These findings justify the use of honey as a
safe and effective dietary supplement to boost male procreation in rabbits.
References
Abduljaleel, M. R., Abdulrazaq, A. W., Jassim, M. M., Abbas, M. F., Alfaris, A. A., Naeem, R. M., Ibrahim, A. A., Mohammed Saleh, W. M., Alrashid, I. M., & Alrafas, H. R. (2025). Understanding the synergistic impact of atropine with xylazine and ketamine on recovery time, heart rate and respiratory rate in male rabbits. Assiut Veterinary Medical Journal, 71(187), 237–246. https://doi.org/10.21608/avmj.2025.341413.1505
Al-Farsi, M. A., & Lee, C. Y. (2008). Nutritional and functional properties of dates: A review. Critical Reviews in Food Science and Nutrition, 48(10), 877–887. https://doi.org/10.1080/10408390701724264
Anyakudo, M. M. C., Balogun, A. J., & Adeniyi, M. O. (2015). Beneficial effects of honey-based diet on glycemic control and reproductive potential in diabetic rats. World Journal of Nutrition and Health, 3(2), 41–46. https://doi.org/10.12691/jnh-3-2-3
Boivin, J., Bunting, L., Collins, J. A., & Nygren, K. G. (2007). International estimates of infertility prevalence and treatment-seeking: Potential need and demand for infertility medical care. Human Reproduction, 22(6), 1506–1512. https://doi.org/10.1093/humrep/dem046
Chan, J. K. (2014). The wonderful colours of the hematoxylin–eosin stain in diagnostic surgical pathology. International Journal of Surgical Pathology, 22(1), 12–32. https://doi.org/10.1177/1066896913517939
El-Aziz, A. A., El-Sayed, M. A., & Kamel, H. (2023). The impact of date molasses supplementation on rabbit reproductive performance. Animal Science Journal, 94(2), e13805. https://doi.org/10.1111/asj.13805
Field, A. (2006). Discovering statistics using SPSS (2nd ed.). Sage.
Hegazi, M. M., Khedher, N. B., & Rjiba, M. (2022). The role of antioxidants in improving fertility and reproductive performance in male rabbits. Veterinary Research Communications, 46(1), 1–10. https://doi.org/10.1007/s11259-021-09827-2
Ibrahim, A. A., Naeem, R. M., Abduljaleel, M. R., Jassim, M. M., Abdulrazaq, A. W., Khaleefah, I. A., & Hashim, A. M. (2025). Effect of platelet-rich fibrin (PRF) from different sources on avulsion wound healing in a rabbit model. Journal of Animal Health and Production, 13(4), 1244–1251. https://doi.org/10.17582/journal.jahp/2025/13.4.1244.1251
Jassim, M. M., Abduljaleel, M. R., Abdulkareem, Z. B., Sanad, N. H., & Alrashid, I. M. H. (2023). Study the effect of the magnetic field on the healing of bone fracture after implant avian bone in femoral bone in rabbits. Advances in Animal and Veterinary Sciences, 11(11), 1779–1784. https://doi.org/10.17582/journal.aavs/2023/11.11.1779.1784
Khan, M. I., Ullah, N., & Bafakeeh, O. T. (2023). Protective effects of honey on oxidative stress in male reproductive health: A review. Journal of Apicultural Research, 62(1), 13–22. https://doi.org/10.1080/00218839.2022.2100833
Mardani, K., Ranjbar, A., & Khosravi, S. (2021). The effect of honey on reproductive hormones and sperm quality in male rabbits. Journal of Animal Physiology and Animal Nutrition, 105(3), 445–453. https://doi.org/10.1111/jpn.13466
Mohsin, T. A., Abduljaleel, M. R., Radhi, A. J., Abbas, M. F., Alrashid, I. M. H., & Khudhair, Z. W. (2025). Histopathological effects of streptomycin treatment on macrophages in lymph nodes, spleen, liver and kidneys of rats. Advances in Animal and Veterinary Sciences, 13(6), 1337–1345. https://doi.org/10.17582/journal.aavs/2025/13.6.1337.1345
Nasr, M. A., & Al-Khalifa, H. S. (2012). Effect of honey on the semen quality of male rabbits. Saudi Journal of Biological Sciences, 19(3), 329–333. https://doi.org/10.1016/j.sjbs.2012.03.004
Orr, H. E., Roughan, J. V., & Flecknell, P. A. (2005). Assessment of ketamine and medetomidine anaesthesia in rabbits. Veterinary Anaesthesia and Analgesia, 32(5), 271–275. https://doi.org/10.1111/j.1467-2995.2005.00205.x
Petrelli, G., & Mantovani, A. (2002). Environmental risk factors and male fertility and reproduction. Contraception, 65(4), 297–300. https://doi.org/10.1016/S0010-7824(02)00288-7
Plas, E., Berger, P., Hermann, M., & Pflüger, H. (2000). Effects of ageing on male fertility. Experimental Gerontology, 35(5), 543–551. https://doi.org/10.1016/S0531-5565(00)00121-7
Salman, T. M., Alagbonsi, I. A., & Olayaki, L. A. (2013). Honey increases sperm count in male albino rats by enhancing testosterone production. Biokemistri, 25(2), 39–44.
Tartibian, B., & Maleki, B. H. (2012). The effects of honey supplementation on seminal plasma cytokines, oxidative stress biomarkers, and antioxidants during 8 weeks of intensive cycling training. Journal of Andrology, 33(3), 449–461. https://doi.org/10.2164/jandrol.111.013292

