Efficacy of Hormonal Injection on Day 11 Post-Mating in Reducing Embryonic Loss and Selected Blood Parameters in Awassi Ewes
DOI:
https://doi.org/10.48165/ijapm.2026.42.01.14Keywords:
Kisspeptin-10; GnRH; hCG; Metabolites; Reproductive Performance; Embryonic Loss; Awassi ewes.Abstract
This study aimed to evaluate the effects of a single hormonal injection of Kisspeptin-10 ,GnRH ,hCG and progesterone administered on day 11 post-mating on embryonic survival, serum progesterone concentration, selected amino acid and fatty acid profiles, and reproductive performance in Awassi ewes. Fifty Awassi ewes (3–4 years old; 40–45 kg body weight) were synchronized using intravaginal sponges containing 20 mg flugestone acetate for 14 days and naturally mated. Ewes were randomly allocated into five groups :first group/ control (2 mL normal saline), second group /Kisspeptin-10 (10 µg/kg BW), third group /GnRH (2.5 mL), fourth group /hCG (0.5 mL), and fifth group / progesterone (0.5 mL), administered on day 11 post-mating. Blood samples were collected on days 34, 51, and 68 of gestation. Results showed significant increase (P<0.05)in progesterone and cholesterol of hormonal treatment on day 51,68 compared to control group. Hormonal treatments significantly increased serum amino acid and fatty acid concentrations (P < 0.01) compared with control. Hormonal treatments improved reproductive performance , fertility, twinning rate compared with control . In conclusion, hormonal administration on day 11 post-mating enhances luteal function ,and may contribute to reduced embryonic loss and improved reproductive efficiency in Awassi ewes.
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