MOLECULAR CHARACTERIZATION AND BIOREMEDIATION ASSESSMENT OF A LEAD-RESISTANT BACTERIUM-DERIVED FROM INDUSTRIAL WASTE USING INDUCTIVELY COUPLED PLASMA OPTCAL EMISSION SPECTROSCOPY
DOI:
https://doi.org/10.48165/abr.2026.28.01.21Keywords:
Bacillus cereus, bioremediation, ICP-OES, lead, physico chemical parametersAbstract
The environmental pollution due to the lead discharge from industrial effluent is a major concern owing to its non-biodegradable and toxic nature. The present study was aimed to isolate, screen, and molecularly characterize a lead resistant bacterium isolated from the industrial effluents in Pune (India). The study was conducted in the years 2022-2024. The effluent samples were analysed for physicochemical characteristics and lead concentrations, both before and after bioremediation treatment. The lead concentration in the samples was quantified by inductively coupled plasma optical emission spectroscopy during a lead reduction study over 48 h. Of the 30 bacterial isolates from the samples collected, five isolates viz., S1, S4, S8, S12 and S14 exhibited resistance up to 1000 ppm. Isolate S8 showed maximum lead reduction (14.80-6.78) in 48 h. Based on the morphobiochemical and molecular characterisation the isolate was identified as a Bacillus cereus (Accession No. PZ327497). The 16S rRNA sequencing and phylogenetic analysis were performed using MEGA11 software. Further, the physicochemical parameters of industrial effluent such as BOD, COD, TSS, and TDS were considerably reduced after treatment. The study demonstrated that B. cereus strain S8 is a potential isolate for the bioremediation of lead polluted industrial effluent.
Downloads
References
APHA. 1995. Standard Methods for the Examination of Water and Wastewater. 19th ed. American Public Health Association, Washington, USA.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol. 215(3):403–410.
Basha SA, Rajaganesh K. 2014. Microbial bioremediation of heavy metals from textile industry dye effluents using isolated bacterial strains. Int J Curr Microbiol Appl Sci. 3:785–794.
Bergey DH, Krieg NR, Holt JG, Williams ST. 1984. Bergey’s Manual of Systematic Bacteriology. Vol. 1. Williams & Wilkins, Baltimore, USA.
Chatterjee S, Mukherjee A, Sarkar A, Roy A. 2012. Bioremediation of lead by lead-resistant microorganisms. Adv Biosci Biotechnol. 3:290–295.
De J, Ramaiah N, Vardanyan L. 2008. Detoxification of toxic heavy metals by marine bacteria highly resistant to mercury. Mar Biotechnol. 10:471–477.
Dekhil B, Hannachi Y, Ghorbel A, Boubaker T. 2011. Removal of lead and cadmium ions from aqueous solutions using the macroalga Caulerpa racemosa. Chem Ecol. 27(3):221–234.
Donald LS. 2003. Environmental Soil Chemistry. Academic Press, San Diego, USA.
Gayatri Y, Raj MS, Vijayalakshmi B. 2017. Biosorption of lead by Bacillus licheniformis isolated from e-waste landfill, Hyderabad, India. Int J Bioassays. 6(2):5240–5243.
Govarthanan M, Mythili R, Selvankumar T, Kamala-Kannan S, Rajasekar A, Chang YC. 2016. Bioremediation of heavy metals using an endophytic bacterium Paenibacillus sp. 3 Biotech. 6:242. doi:10.1007/s13205-016-0560-1.
Held T, Don H. 2000. In situ remediation. Biotechnology. 11B:350–370.
Idrees M, Ali S, Rehman A, Hussain SZ, Bukhari DA. 2023. Uptake of lead by bacteria isolated from industrial effluents and their potential use in bioremediation of wastewater. Saudi J Biol Sci. 30(8):103740. doi:10.1016/j.sjbs.2023.103740.
Kadirvelu K, Thamaraiselvi K, Namasivayam C. 2001. Removal of heavy metals from industrial wastewater by adsorption onto activated carbon. Bioresour Technol. 76:63–65.
Kalita D, Joshi SR. 2017. Bioremediation of lead by exopolysaccharide-producing bacterium. Biotechnol Rep. 16:48–57.
Kang CH, Kwon YJ, So JS. 2016. Bioremediation of heavy metals using bacterial mixtures. Ecol Eng. 89:64–69.
Khaleel RI, Ismail N, Ibrahim MH. 2013. Impact of wastewater treatments on seed germination and biochemical parameters of Abelmoschus esculentus. Procedia Soc Behav Sci. 91:453–460.
Kleijntjens RH, Luyben KC. 2000. Bioremediation. Biotechnology. 11B:329–347.
Kumar M, Puri A. 2012. Review of permissible limits of drinking water. Indian J Occup Environ Med. 16:40–44.
Lezcano JM, González F, Ballester A, Blázquez ML, Muñoz JA, García-Balboa C. 2011. Sorption and desorption of Cd, Cu and Pb using biomass. J Environ Manage. 92(10):2666–2674.
Ma Y, Oliveira RS, Wu L, Luo Y, Rajkumar M, Rocha I, et al. 2015. Role of PGPR in rhizoremediation. J Toxicol Environ Health A. 78(14):931–944.
Marin AB, Ortuño JF, Aguilar MI, Meseguer VF, Saez J, Lloréns M. 2010. Binding of heavy metals by orange waste. Biochem Eng J. 53(1):2–6.
Marzan LW, Hossain M, Mina SA, Akter Y, Chowdhury AM. 2017. Heavy metal resistant bacteria from tannery effluent. Egypt J Aquat Res. 43(1):65–74.
Montgomery DC. 2017. Design and Analysis of Experiments. 9th ed. John Wiley & Sons, Hoboken, New Jersey, USA.
Salehizadeh H, Shojaosadati S. 2003. Removal of metal ions using polysaccharides from Bacillus firmus. Water Res. 37(17):4231–4235.
Senthil Kumar P. 2014. Adsorption of lead ions using natural waste. Environ Prog Sustain Energy. 33:55–64.
Sood PP, Prakash R. 1998. Heavy Metal Pollution, Toxication and Chelation. M.D. Publications, New Delhi, India.
Tamura K, Stecher G, Kumar S. 2021. MEGA11 software. Mol Biol Evol. 38(7):3022–3027.
Van Deuren J, Lloyd T, Chhetry S, Raycharn L, Peck J. 2002. Remediation Technologies Screening Matrix and Reference Guide. Federal Remediation Technologies Roundtable. 5:1–4.
WHO. 2017. Guidelines for Drinking-Water Quality. 4th ed. World Health Organization, Geneva, Switzerland.
Zar JH. 2010. Biostatistical Analysis. 5th ed. Pearson Education, New Jersey, USA.
Zhiqi H, Reiske HR, Wilson DB. 1999. Cadmium uptake in Lactobacillus plantarum. Appl Environ Microbiol. 65:4741–4745.
